Polymer mesh scaffold combined with cell-derived ECM for osteogenesis of human mesenchymal stem cells

Abstract

BackgroundTissue-engineered scaffold should mimic the structure and biological function of the extracellular matrix and have mechanically supportive properties for tissue regeneration. In this study, we utilized a PLGA/PLA mesh scaffold, coated with cell-derived extracellular matrix (CDM) and assessed its potential as an osteogenic microenvironment for human umbilical cord blood-derived mesenchymal stem cells (UCB-MSCs). CDM was obtained by decellularization of in vitro-cultured type I collagen overexpressing (Col I -293 T-DK) cells. Test groups are mesh itself (control), fibronectin-coated (FN-mesh), and CDM-coated mesh scaffold (CDM-mesh). CDM was then solubilized and used for scaffold coating.ResultsCDM was successfully collected and applied to mesh scaffolds. The presence of CDM was confirmed via SEM and FN immunofluorescence. After then, UCB-MSCs were seeded into the scaffolds and subjected to the induction of osteogenic differentiation for 21 days in vitro. We found that the seeded cells were viable and have better proliferation activity on CDM-mesh scaffold. In addition, when osteogenic differentiation of UCB-MSCs was examined for up to 21 days, alkaline phosphatase (ALP) activity and osteogenic marker (COL I, ALP, osteocalcin, bone sialoprotein) expression were significantly improved with UCB-MSCs when cultured in the CDM-mesh scaffold compared to the control and FN-mesh.ConclusionPolymer mesh scaffold incorporated with CDM can provide UCB-MSCs with a better microenvironment for osteogenesis in vitro.

Cultured cell‐derived extracellular matrices to enhance the osteogenic differentiation and angiogenic properties of human mesenchymal stem/stromal cells

“…In fact, ECM proteins can be secreted by cells cultured in vitro and then decellularized to create the cell‐derived ECM (Hoshiba, Cho, Murakawa, Okahata, & Akaike, ). Cell‐derived ECM has several advantages over tissue‐derived ECM, because it is easy to eliminate pathogen content and it can be derived from autologous cells (Lu et al, ; Noh et al, ). In fact, cell‐derived ECM can be created in vitro using patient‐specific cells.…”

“…Cell-derived ECM has several advantages over tissue-derived ECM, because it is easy to eliminate pathogen content and it can be derived from autologous cells (Lu et al, 2011;Noh et al, 2016). In fact, cellderived ECM can be created in vitro using patient-specific cells.…”

Photo-bactericidal Properties of Hydroxyapatite Implant Surface Coating

“…An aqueous solution of H 3 PO 4 was added very slowly to a suspension of Ca(OH) 2 . During reaction, temperature of the suspension was maintained at 80°C.…”

“…The HAp powder was prepared by precipitation between Ca(OH) 2 and H 3 PO 4 according to the following reaction:…”

“…The research of the organic compounds and synthetic materials integration processes in biological tissue is crucial for improving the quality of life of patients in need of regeneration or replacement of tissue defects [1][2][3][4][5][6]. Implant techniques have found application in many areas of medicine (orthopedics, neurosurgery, cardiac surgery, dentistry, traumatology, etc.).…”