Polymer Immobilization to Alkylchlorosilane Organic Monolayer Films Using Sequential Derivatization Reactions

Mao, G.; Castner, David G.; Grainger, David W.

doi:10.1021/cm960450j

Cited by 32 publications

(23 citation statements)

References 51 publications

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“…X‐ray photoelectron spectroscopy (XPS) experiments were performed on a Surface Science SSX‐100 spectrometer equipped with a monochromatic Al Kα source, hemispheric analyzer, and a multichannel detector, as previously detailed 26…”

Section: Methodsmentioning

confidence: 99%

Correlating fibronectin adsorption with endothelial cell adhesion and signaling on polymer substrates

Koenig

Gambillara

Grainger

2002

J Biomedical Materials Res

101

106

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Fibronectin (Fn) adsorption was studied on different commercial polymer surface chemistries, including tissue culture polystyrene (TCPS), bacteriologic polystyrene (BPS), fluoropolymer Teflon AF, and poly-L-lactide (PLLA). Antibody probes detected the availability of Fn's cell binding domain on adsorbed Fn in the competitive presence and absence of bovine serum albumin (BSA). Domain availability was highest for Fn adsorbed on TCPS, especially in the presence of either serum albumin or dilute serum. Attachment and growth efficiencies for human umbilical venous endothelial cells (HUVECs) cultured on surfaces preadsorbed with Fn in serum and serum-free media correlated with antibody cell-binding domain availability: TCPS > BPS, Teflon AF > PLLA. Intracellular signaling from the GTPase, RhoA, was highest (RhoA:RhoGDI inhibitor ratio) in cells cultured on the Teflon AF surfaces, indicating that despite lower attached cell numbers on Teflon AF compared to TCPS, cell signaling remained activated after 24 h of growth. Up-regulated cellular Fn mRNA messages, assessed using RT-PCR techniques, supported HUVECs' producing the endogenous extracellular matrix (ECM) protein Fn in order to attach and survive on the suboptimal Teflon AF culture surfaces.

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Section: Methodsmentioning

confidence: 99%

Correlating fibronectin adsorption with endothelial cell adhesion and signaling on polymer substrates

Koenig

Gambillara

Grainger

2002

J Biomedical Materials Res

101

106

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“…Spectra were calibrated to the CH 3 C , C 2 H 3 C , C 3 H 5 C and C 7 H 7 C peaks prior to further analysis. 5 The positive ion data included the nitrogen-containing peaks from the underlying PEI and acetaldehyde plasma polymer substrate, in addition to the oxygen-containing peaks from the CMD graft, providing all of the information necessary for discriminating between these two grafts.…”

Section: Time-of-flight Secondary Ion Mass Spectrometrymentioning

confidence: 99%

“…4 There has been a tendency to characterize each stage of a polymer grafting process as an isolated layer that does not intercalate into or significantly interact with the adjacent polymer layer. 5,6 In the instances where covalent coupling reactions are utilized, interactions between layers may be more complex. The individual characteristics of each polymer (e.g.…”

Section: Introductionmentioning

confidence: 99%

Characterization of sequentially grafted polysaccharide coatings using time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) and principal component analysis (PCA)

McArthur

Wagner

Hartley

et al. 2002

Surface & Interface Analysis

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Sequentially grafted polymer layers are playing an increasingly important role in a variety of biomedical applications. Characterization of the chemistry and structure of these coatings has typically followed a layer-by-layer approach, ignoring potential interactions between the adjacent layers and the effects that these interactions may have on the resulting physicochemical characteristics of the surface. Using a sequential grafting protocol to couple the polysaccharide carboxymethyldextran (CMD), previous surface force and x-ray photoelectron spectroscopy studies have suggested that there was some intercalation of the CMD and intermediate poly(ethyleneimine) (PEI) layers. We have utilized the surface and chemical specificity of time-of-flight secondary ion mass spectrometry (ToF-SIMS) to investigate the intercalation of the polymer layers. Owing to the complexity of the data, principal component analysis (PCA) was used to interpret the spectra of the grafted polymer films. The results of the PCA indicated that variations between spectra could be related directly to the carboxyl density (1 : 2 or 1 : 30 carboxyls per glucose unit) of the CMD. Variations between the spectra were shown to be due to the presence of nitrogen-rich species from the PEI at the outermost surface in the CMD 1 : 30 samples, whereas the CMD 1 : 2 spectra contained oxygen-rich species related to the presence of the polysaccharide. These results enabled a model to be developed combining the new data with the previous studies to show the carboxyl-density-dependent intercalation of the CMD and PEI layers in this polymer graft. The application of ToF-SIMS and PCA allowed conclusive determination of the surface structure of this complex grafted polymer layer.

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“…(98) Also, polymeric molecules with silane anchor groups have been assembled onto hydroxylated surfaces. (105) In fact, anchor groups can be used to form patterned organic monolayers on surfaces. The surface is first patterned by fabricating areas of different materials (Au, hydroxyl groups, oxides).…”

Section: Silane Monolayersmentioning

confidence: 99%

Chemical Modification of Surfaces

Castner

2002

Specimen Handling, Preparation, and Treatments in Surface Characterization

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Polymer Immobilization to Alkylchlorosilane Organic Monolayer Films Using Sequential Derivatization Reactions

Cited by 32 publications

References 51 publications

Correlating fibronectin adsorption with endothelial cell adhesion and signaling on polymer substrates

Correlating fibronectin adsorption with endothelial cell adhesion and signaling on polymer substrates

Characterization of sequentially grafted polysaccharide coatings using time‐of‐flight secondary ion mass spectrometry (ToF‐SIMS) and principal component analysis (PCA)

Chemical Modification of Surfaces

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