Polymer–Clay Nanocomposite-Based Acetylcholine esterase Biosensor for Organophosphorous Pesticide Detection

Sarkar, Tamal; Narayanan, Neethu; Solanki, Pratima R.

doi:10.1007/s41742-017-0052-0

Cited by 12 publications

(4 citation statements)

References 36 publications

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“…In this context, it is important to highlight the advantages of using electrochemical biosensors given their higher selectivity and specificity; shorter analysis and sample preparation time; lower sample volume; and less expensive equipment. Despite the enzymatic costs, HRP-based enzymatic biosensors have been reported for the determination of the polyphenol content of yerba mate and green coffee (20), as well as in environmental samples (wastewater from coffee washing) [51], antioxidants in food matrices [56], and endocrine-disrupting compounds in pharmacological products [57].…”

Section: Determination Of Polyphenols In Pennyroyal and Lemon Verbena...mentioning

confidence: 99%

“…An alternative is the preparation of the nanocomposites of clays and biopolymers such as CHIT, cellulose and its derivatives, starch, alginate, or pectin, among others. Nanocomposites of Mt–biopolymers allow for the formation of films suitable for functionalizing transducers, for example, electrodes, and provide free functional groups for biomolecule binding, allowing long-term stable structures to be obtained [ 20 ]. The addition of polycations increases the mechanical stability of clay films and could improve the electrical conductivity at the interface.…”

Section: Introductionmentioning

confidence: 99%

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Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols

Piccoli,

Gulotta,

Montenegro

et al. 2024

Biosensors

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Glucosamine–chitosan synthesized by the Maillard reaction was combined with montmorillonite to obtain a nanohybrid composite to immobilize horseradish peroxidase. The material combines the advantageous properties of clay with those of the chitosan derivative; has improved water solubility and reduced molecular weight and viscosity; involves an eco-friendly synthesis; and exhibits ion exchange capacity, good adhesiveness, and a large specific surface area for enzyme adsorption. The physicochemical characteristics of the composite were analyzed by infrared spectroscopy and X-ray diffraction to determine clay–polycation interactions. The electrochemical response of the different polyphenols to glassy carbon electrodes modified with the composite was evaluated by cyclic voltammetry. The sensitivity and detection limit values obtained with the biosensor toward hydroquinone, chlorogenic acid, catechol, and resorcinol are (1.6 ± 0.2) × 102 µA mM−1 and (74 ± 8) nM; (1.2 ± 0.1) × 102 µA mM−1 and (26 ± 3) nM; (16 ± 2) µA mM−1 and (0.74 ± 0.09) μM; and (3.7± 0.3) µA mM−1 and (3.3 ± 0.2) μM, respectively. The biosensor was applied to quantify polyphenols in pennyroyal and lemon verbena extracts.

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Section: Determination Of Polyphenols In Pennyroyal and Lemon Verbena...mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols

Piccoli,

Gulotta,

Montenegro

et al. 2024

Biosensors

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“…Enzymatic biosensors based on CLME can be used for the determination of pesticides through their inhibitory action on enzyme activity. In the last decade, the main studies have been centered in the determination of glyphosate and chlorpyriphos (see Table 4) [49][50][51]. For example, a biosensor based on atemoya peroxidase immobilized into a modified nanoclay (montmorillonite containing 0.5-5 wt.% aminopropyltriethoxysilane and 15-35 wt.% octadecylamine) was developed [49].…”

Section: Nanoclay-based Sensors For Environmental Applicationsmentioning

confidence: 99%

“…For the detection of chlorpyriphos, several nanoclays functionalized with chitosan [50] or metallic NPs [52] were employed for the fabrication of enzymatic biosensors via immobilization of acetylcholinesterase (AChE) [50,51].…”

Section: Nanoclay-based Sensors For Environmental Applicationsmentioning

confidence: 99%

New Trends in Nanoclay-Modified Sensors

et al. 2021

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Nanoclays are widespread materials characterized by a layered structure in the nano-scale range. They have multiple applications in diverse scientific and industrial areas, mainly due to their swelling capacity, cation exchange capacity, and plasticity. Due to the cation exchange capacity, nanoclays can serve as host matrices for the stabilization of several molecules and, thus, they can be used as sensors by incorporating electroactive ions, biomolecules as enzymes, or fluorescence probes. In this review, the most recent applications as bioanalyte sensors are addressed, focusing on two main detection systems: electrochemical and optical methods. Particularly, the application of electrochemical sensors with clay-modified electrodes (CLME) for pesticide detection is described. Moreover, recent advances of both electrochemical and optical sensors based on nanoclays for diverse bioanalytes’ detection such as glucose, H2O2, organic acids, proteins, or bacteria are also discussed. As it can be seen from this review, nanoclays can become a key factor in sensors’ development, creating an emerging technology for the detection of bioanalytes, with application in both environmental and biomedical fields.

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Clay Based Biopolymer Nanocomposites and Their Applications in Environmental and Biomedical Fields

Sangeetha¹,

Vinodhini²,

Sudha³

2019

Sustainable Polymer Composites and Nanocomposites

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Polymer–Clay Nanocomposite-Based Acetylcholine esterase Biosensor for Organophosphorous Pesticide Detection

Cited by 12 publications

References 36 publications

Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols

Immobilization of Horseradish Peroxidase onto Montmorillonite/Glucosamine–Chitosan Composite for Electrochemical Biosensing of Polyphenols

New Trends in Nanoclay-Modified Sensors

Clay Based Biopolymer Nanocomposites and Their Applications in Environmental and Biomedical Fields

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