Polymer-based technology platform for robust electrochemical sensing using gold microelectrodes

Kuphal, Mathias; Mills, Christopher A.; Korri-Youssoufi, Hafsa; Samitier, J.

doi:10.1016/j.snb.2011.10.032

Cited by 10 publications

(7 citation statements)

References 45 publications

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“…Figure 2B shows the circuit used to model the EIS of the microelectrodes, which was chosen in this particular case because of the use of the redox couple K 3 [Fe(CN) 6 ]/K 4 [Fe(CN) 6 ]. 40 Aptamer modification in both materials after exposing to the cell extracts showed changes in R ct with time; however, these changes showed a significant deviation between them when analyzed for different microelectrodes compared between them giving high error bars. The change in R ct was attributed to the chemical changes in the surface of the microelectrodes produced by the interactions between the highly diverse mixture of biological molecules of the cell extract with the surface of the microelectrodes that cover the surface reducing the electron transfer and redox reaction.…”

Section: ■ Results and Discussionmentioning

confidence: 94%

“…This difference in surface modification can be seen in the Nyquist plots in Figure S1C,D where carbon fiber microelectrodes present more than one time constant while platinum microelectrodes only present one constant, which is due to the non-uniform current distribution at the electrode surface. 40 Moreover, the lower frequencies that are impacted in platinum are related to limitation in mass transport, which is expected given the high charge transfer seen in metal microelectrodes compared to carbon fiber.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

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Measurement of Neuropeptide Y Using Aptamer-Modified Microelectrodes by Electrochemical Impedance Spectroscopy

et al. 2020

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Aptamer-modified microelectrodes for Neuropeptide Y measurement by electrochemical impedance spectroscopy was described here. The advantages of using carbon fiber or platinum microelectrodes are because they are promising materials with high electrical conductivity, chemical stability, and high surface area that can be easily modified on their surface. The immobilization and biofouling were studied and compared using EIS. Moreover, the adsorption of NPY to the aptamer-modified microelectrodes was also demonstrated by EIS. Changes of −ω*Z imag, an impedance factor that gives information of the capacitance, is directly correlated with concentrations. A widely linear range was obtained from 10 to 1000 ng/mL of NPY. This method was able to detect NPY without performing a redox reaction by adsorption at the surface of the microelectrodes, with the specificity provided by aptamer functionalization of the microelectrode surface.

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Section: ■ Results and Discussionmentioning

confidence: 94%

Section: ■ Results and Discussionmentioning

confidence: 99%

Measurement of Neuropeptide Y Using Aptamer-Modified Microelectrodes by Electrochemical Impedance Spectroscopy

et al. 2020

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“…Also, microelectrodes have been manufactured by combining microphotolithography and screen-printing technology to produce microbiosensors capable of monitoring glucose metabolism in 96-well format cell cultures [11]. The majority of approaches to microelectrode fabrication are based on gold electrodes and conventional fabrication techniques such as photolithography with the main disadvantage of production cost [12][13][14][15][16].…”

Section: Introductionmentioning

confidence: 99%

Design and development of novel screen-printed microelectrode and microbiosensor arrays fabricated using ultrafast pulsed laser ablation

Liébana

Jones

Drago

et al. 2016

Sensors and Actuators B: Chemical

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Design and development of novel screen-printed microelectrode and microbiosensor arrays fabricated using ultrafast pulsed laser ablation. Sensors and Actuators B: Chemical, 231. pp. 384-392. ISSN 0925-4005 Available from: http://eprints.uwe.ac.uk/28503We recommend you cite the published version. The publisher's URL is: http://dx.doi.org/10. 1016/j.snb.2016.02.142 Refereed: Yes (no note) Disclaimer UWE has obtained warranties from all depositors as to their title in the material deposited and as to their right to deposit such material. UWE makes no representation or warranties of commercial utility, title, or fitness for a particular purpose or any other warranty, express or implied in respect of any material deposited. UWE makes no representation that the use of the materials will not infringe any patent, copyright, trademark or other property or proprietary rights. UWE accepts no liability for any infringement of intellectual property rights in any material deposited but will remove such material from public view pending investigation in the event of an allegation of any such infringement. PLEASE SCROLL DOWN FOR TEXT. Accepted ManuscriptTitle: Design and development of novel screen-printed microelectrode and microbiosensor arrays fabricated using ultrafast pulsed laser ablation This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.  The generic platform combines screen-printing and ultrafast pulsed laser technologies. The activity of the enzymatic system was retained after processing by pulse laser ablation. Steady-state responses were obtained using chronoamperometry. A prototype glucose microbiosensor array was developed. . This study provides a new fabrication method for microelectrode and microbiosensor arrays capable for the first time to retain the activity of the enzymatic system after processing by pulse laser ablation.

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“…The electrode layer comprised a five element IDE array (30µm finger width and spacing), four resistive elements for fluid localisation and two platinum temperature sensor elements on a 125µM PEN sheet. Gold electrodes were fabricated by physical vapour deposition (PVD) by sputtering a 250 nm gold layer (Au) with subsequent patterning using photolithography as described previously (Kuphal et al, 2012). An extra metal layer (e.g.…”

Section: Microfluidic Cartridge With Ide Arraymentioning

confidence: 99%

Impedimetric array in polymer microfluidic cartridge for low cost point-of-care diagnostics

Lakey

Ali

Scott

et al. 2019

Biosensors and Bioelectronics

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Impedimetric array in polymer microfluidic cartridge for low cost point-of-care diagnostics, Biosensors and Bioelectronic, https://doi.ABSTRACT Deep Vein Thrombosis and pulmonary embolism (DVT/PE) is one of the most common causes of unexpected death for hospital in-patients. D-dimer is used as a biomarker within blood for the diagnosis of DVT/PE. We report a low-cost microfluidic device with a conveniently biofunctionalised interdigitated electrode (IDE) array and a portable impedimetric reader as a point-of-care (POC) device for the detection of Ddimer to aid diagnosis of DVT/PE. The IDE array elements, fabricated on a polyethylenenaphtalate (PEN) substrate, are biofunctionalised in situ after assembly of the microfluidic device by electropolymerisation of a copolymer of polypyrrole to which is immobilised a histidine tag anti-D-Dimer antibody. The most consistent copolymer films were produced using chronopotentiometry with an applied current of 5A for a period of 50 seconds using a two-electrode system. The quality of the biofunctionalisation was monitored using optical microscopy, chronopotentiometry curves and impedimetric analysis. Measurement of clinical plasma sample with a Ddimer at concentration of 437 ng/mL with 15 biofunctionalised IDE array electrodes gave a ratiometric percentage of sample reading against the blank with an average value of 124±15 at 95% confidence. We have demonstrated the concept of a low cost disposable microfluidic device with a receptor functionalised on the IDE array for impedimetric detection towards POC diagnostics. Changing the receptor on the IDE array would allow this approach to be used for the direct detection of a wide range of analytes in a low cost manner.

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Polymer-based technology platform for robust electrochemical sensing using gold microelectrodes

Cited by 10 publications

References 45 publications

Measurement of Neuropeptide Y Using Aptamer-Modified Microelectrodes by Electrochemical Impedance Spectroscopy

Measurement of Neuropeptide Y Using Aptamer-Modified Microelectrodes by Electrochemical Impedance Spectroscopy

Design and development of novel screen-printed microelectrode and microbiosensor arrays fabricated using ultrafast pulsed laser ablation

Impedimetric array in polymer microfluidic cartridge for low cost point-of-care diagnostics

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