Polylactide nanofibers loaded with vitamin B6 and polyphenols as bioactive platform for tissue engineering

Llorens, Elena; Valle, Luís J. del; Díaz, Angélica; Casas, María Teresa; Puiggalı́, Jordi

doi:10.1007/s13233-013-1090-x

Cited by 41 publications

(42 citation statements)

References 41 publications

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“…It is also important to remark that the residual H O concentration was monitored during all the reaction time span due to its well-known cytotoxic effect via oxidative stress (Llorens et al 2013 ). The reagent concentration after 30 min of reaction was negligible for all experimental sets studied, except experiment D. The results are in accordance with the literature (Baciocchi et al 2003 ) and denote the fast consumption of H O reagent following a pseudo-first-order constant proposed for H O decomposition.…”

Section: Figsupporting

confidence: 87%

“…Afterward, the media were aspired and the samples in half-dilution were prepared with a culture medium, which was evaluated for cellular toxicity by exposing cells to direct contact with the samples prepared. Then, the plates were incubated for another 24 h. Finally, cell viability was determined by the MTT assay (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium; 5 mg mL ) (Mosmann 1983 ;Llorens et al 2013 ), and then, the mortality was calculated for each sample as the difference between the viability of the control (medium without BPA) and the sample. The samples were evaluated using five replicates, and the results were averaged and graphically represented.…”

Section: Antimicrobial Testmentioning

confidence: 99%

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Study of the degradation performance (TOC, BOD, and toxicity) of bisphenol A by the photo-Fenton process

Pérez-Moya¹,

Kaisto

Navarro³

et al. 2016

Environ Sci Pollut Res

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Degradation of bisphenol A (BPA, 0.5 L, 30 mg L ) was studied by photoFenton treatment, while Fenton reagents were variables. The efficiency of the degradation process was evaluated by the reduction of total organic carbon (TOC), the biochemical oxygen demand (BOD), and toxicity. For toxicity analysis, bacterial methods were found infeasible, but the in vitro assay of VERO cells culture was successfully applied. Experiments according to a 2 design of experiments (DOE) with star points and three center points for statistical validity allowed selecting those process conditions (Fe(II) and H O load) that maximized the process performance. Photo-Fenton process effectively eliminated BPA and partly degraded its by-products (residual TOC <15 %) under substoichiometric H O dose (100.62 mg L ) and at least 4 mg L Fe(II), after a 90-min treatment. All

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Section: Figsupporting

confidence: 87%

Section: Antimicrobial Testmentioning

confidence: 99%

Study of the degradation performance (TOC, BOD, and toxicity) of bisphenol A by the photo-Fenton process

Pérez-Moya¹,

Kaisto

Navarro³

et al. 2016

Environ Sci Pollut Res

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“…Thus, diameters could be easily varied from 100 nm (i.e., nanometer scale) to 2 m (i.e., micrometer scale) by using dimethyl formamide and chloroform-acetone (2:1 v/v) mixture as solvents, respectively, when polymer concentration was kept at 10% (w/v). 29,30 Nevertheless, PLA concentration also played a determinant role because nanofibers could again be obtained when it was lower than 5% (w/v). 31 The above conclusions were taken into account in this study since a micrometer scale was preferred to detect release differences between drugs and from distinct PLA matrices.…”

Section: Resultsmentioning

confidence: 99%

“…These values were close to those deduced for the corresponding commercial pellet samples and clearly higher than those obtained from the heating run performed with commercial samples after being slowly cooled from the melt state. 29 This feature suggests that electrospun PLA fibers can easily crystallize during a subsequent heating run as a consequence of a remarkable degree of molecular orientation that could be attained in the electrospinning process. The high relaxation peak observed for the PLA 4032D electrospun sample, which indicates the great ability of this PLA grade to achieve the equilibrium thermodynamic conditions characterized by higher stiffness and lower enthalpy and volume, is also worth noting.…”

Section: Resultsmentioning

confidence: 99%

Electrospun scaffolds of polylactide with a different enantiomeric content and loaded with anti-inflammatory and antibacterial drugs

2015

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Polylactide (PLA) electrospun microfibers were prepared and loaded with triclosan (TCS), ketoprofen (KTP), or their combination to obtain multifunctional scaffolds with bactericide and anti-inflammatory properties. Continuous and porous fibers with diameters in the micrometer scale and a unimodal distribution were successfully attained using a dual-electrospinning technique. Dual drug-loaded scaffolds showed a peculiar release that was in contrast to the single drug-loaded systems, which suggested the establishment of intermolecular interactions that delayed TCS and KTP release. Antimicrobial activity of all TCS-loaded electrospun scaffolds was demonstrated against E. coli and M. luteus bacteria; and furthermore, KTP-loaded samples slightly showed bactericide activity. Biocompatibility of scaffolds was evaluated by adhesion and proliferation assays, and interestingly, the dual drug-load systems were able to support high TCS doses without adverse effects.

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“…Cos-7 and Vero cells were cultured in Dulbecco's modified Eagle medium (DMEM) as previously reported [52].…”

Section: Cell Adhesion and Proliferation Assaysmentioning

confidence: 99%

New poly(ester urea) derived from l-leucine: Electrospun scaffolds loaded with antibacterial drugs and enzymes

Díaz

Valle

Tugushi

et al. 2015

Materials Science and Engineering: C

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Electrospun scaffolds from an amino acid containing poly(ester urea) (PEU) were developed as promising materials in the biomedical field and specifically in tissue engineering applications. The selected poly(ester urea) was obtained with a high yield and molecular weight by reaction of phosgene with a bis(α-aminoacyl)-α,ω-dioldiester monomer. The polymer having L-leucine, 1,6-hexanediol and carbonic acid units had a semicrystalline character and relatively high glass transition and melting temperatures. Furthermore it was highly soluble in most organic solvents, an interesting feature that facilitated the electrospinning process and the effective incorporation of drugs with bactericidal activity (e.g. biguanide derivatives such as clorhexidine and polyhexamethylenebiguanide) and enzymes (e.g. α-chymotrypsin) that accelerated the degradation process. Continuous micro/nanofibers were obtained under a wide range of processing conditions, being diameters of electrospun fibers dependent on the drug and solvent used. Poly(ester urea) samples were degradable in media containing lipases and proteinases but the degradation rate was highly dependent on the surface area, being specifically greater for scaffolds with respect to films. The high hydrophobicity of new scaffolds had repercussions on enzymatic degradability since different weight loss rates were found depending on how samples were exposed to the medium (e.g. forced or non-forced immersion). New scaffolds were biocompatible, as demonstrated by adhesion and proliferation assays performed with fibroblast and epithelial cells.

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Polylactide nanofibers loaded with vitamin B6 and polyphenols as bioactive platform for tissue engineering

Cited by 41 publications

References 41 publications

Study of the degradation performance (TOC, BOD, and toxicity) of bisphenol A by the photo-Fenton process

Study of the degradation performance (TOC, BOD, and toxicity) of bisphenol A by the photo-Fenton process

Electrospun scaffolds of polylactide with a different enantiomeric content and loaded with anti-inflammatory and antibacterial drugs

New poly(ester urea) derived from l-leucine: Electrospun scaffolds loaded with antibacterial drugs and enzymes

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