Polyisocyanide hydrogels with tunable nonlinear elasticity mediate liver carcinoma cell functional response

Liu, Zixin; Fu, Jingxuan; Yuan, Hongbo; Ma, Biao; Cao, Zhanshuo; Chen, Yafei; Xing, Chengfen; Niu, Xuezhi; Li, Ning; Wang, Hui; An, Hailong

doi:10.1016/j.actbio.2022.06.022

Cited by 11 publications

(6 citation statements)

References 44 publications

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“…218,219 These mechanical properties are attributed to the mechanical asymmetry of fibers that are significantly more resistant to stretching than to bending. [220][221][222][223] Currently, studies of nonlinear mechanical properties of fibrillar networks are mainly conducted for biological fibrillar NCGs. 224 Man-made engineered filamentous NCGs would offer the ability to perform fundamental research on fibrillar networks, however studies of synthetic hydrogels recapitulating the mechanics of biological fibrous networks are currently limited to NCGs formed from polyisocyanopeptide bundles.…”

Section: Exploration Of Structure-dependent Properties Of Ncgsmentioning

confidence: 99%

“…224 Man-made engineered filamentous NCGs would offer the ability to perform fundamental research on fibrillar networks, however studies of synthetic hydrogels recapitulating the mechanics of biological fibrous networks are currently limited to NCGs formed from polyisocyanopeptide bundles. 222,223 Furthermore, fibrillar NCGs may acquire structural anisotropy, that is, the variation in structure along a particular axis or several axes of the gel. Structural anisotropy can be induced by fabricating NCGS using microfluidics, extrusion-based 3D printing, electrospinning, or freeze-casting.…”

Section: Exploration Of Structure-dependent Properties Of Ncgsmentioning

confidence: 99%

Section: Summary and Perspectivesmentioning

confidence: 99%

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Design, characterization and applications of nanocolloidal hydrogels

Morozova,

Gevorkian,

Kumacheva

2023

Chem. Soc. Rev.

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Section: Exploration Of Structure-dependent Properties Of Ncgsmentioning

confidence: 99%

Section: Exploration Of Structure-dependent Properties Of Ncgsmentioning

confidence: 99%

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Design, characterization and applications of nanocolloidal hydrogels

Morozova,

Gevorkian,

Kumacheva

2023

Chem. Soc. Rev.

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“…[14,15] The mechanical properties of the gel are readily modified by changing the polymer concentration (like any hydrogel) and molecular weight, [16] but, more importantly, also by external stimuli such as temperature [17] and magnetic fields [18,19] that exploit the strain-stiffing character of the material. Once decorated with cell-adhesive peptides, such as the commonly used arginine-glycine-aspartic acid motive (abbreviated RGD), the gel turns into a versatile and signal-lean 3D cell culture platform for a wide variety of cells, including stem cells, [20][21] fibroblasts, [22] kidney cells, [23] and many others, [24][25][26][27][28][29][30] as well as organoids. [31][32][33] Additionally, initial studies also show potential for PIC gels for in vivo [34,35] applications.…”

Section: Introductionmentioning

confidence: 99%

A Novel In Vitro Disease Model for Systemic Sclerosis Using Polyisocyanide Hydrogels

Kumari

Wubs²,

Caam

et al. 2022

Advanced Therapeutics

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Systemic sclerosis (SSc) is a rare autoimmune disease with limited treatment options that is characterized by fibrosis in various organs. To screen the effectiveness of new therapies, there is an urgent need for reliable in vitro models. Key is that diseased cells' characteristics are maintained, which is challenging in currently used setups. In this study, an in vitro 3D culture system is described using the biocompatible polyisocyanide (PIC‐RGD) hydrogel and SSc patient‐derived fibroblasts from affected (lesional cells) and from healthy‐skin (healthy cells). In contrast to the standard collagen‐coated 2D cultures, the cells in the 3D PIC‐RGD gels maintain the native phenotype and functionality of the primary cells. The functionality of the model is studied in the presence of the fibrosis stimulator transforming growth factor β1 (TGFβ1) and the suppressor tumor necrosis factor (TNFα). In this study, it is observed that lesional cells have a stronger fibrotic character with increased contraction, proliferation, and expression of collagen, and myofibroblast markers α‐smooth muscle actin and fibroblast activation protein. The high tunability of the hydrogel, which can maintain the native functionality of fibroblasts in in vitro cultures, delivers a crucial step in developing these materials into an effective tool for personalized medicine approaches of SSc patients.

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“…PIC polymers grafted with celladhesive peptides RGD were synthesized using methods as previously reported. 31 PIC hydrogels are thermally responsive and reversible, showing sol−gel transition upon cooling or warming, and their storage moduli increase with increasing temperature (Figure 1a,d). Thus, they are easy to apply and remove as a dressing material, especially friendly for large and complicated wounds.…”

mentioning

confidence: 99%

Microenvironment with NIR-Controlled ROS and Mechanical Tensions for Manipulating Cell Activities in Wound Healing

Sun,

Jin,

Bao

et al. 2024

Nano Lett.

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The extracellular matrix (ECM) orchestrates cell behavior and tissue regeneration by modulating biochemical and mechanical signals. Manipulating cell–material interactions is crucial for leveraging biomaterials to regulate cell functions. Yet, integrating multiple cues in a single material remains a challenge. Here, near-infrared (NIR)-controlled multifunctional hydrogel platforms, named PIC/CM@NPs, are introduced to dictate fibroblast behavior during wound healing by tuning the matrix oxidative stress and mechanical tensions. PIC/CM@NPs are prepared through cell adhesion-medicated assembly of collagen-like polyisocyanide (PIC) polymers and cell-membrane-coated conjugated polymer nanoparticles (CM@NPs), which closely mimic the fibrous structure and nonlinear mechanics of ECM. Upon NIR stimulation, PIC/CM@NPs composites enhance fibroblast cell proliferation, migration, cytokine production, and myofibroblast activation, crucial for wound closure. Moreover, they exhibit effective and toxin removal antibacterial properties, reducing inflammation. This multifunctional approach accelerates healing by 95%, highlighting the importance of integrating biochemical and biophysical cues in the biomaterial design for advanced tissue regeneration.

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Polyisocyanide hydrogels with tunable nonlinear elasticity mediate liver carcinoma cell functional response

Cited by 11 publications

References 44 publications

Design, characterization and applications of nanocolloidal hydrogels

Design, characterization and applications of nanocolloidal hydrogels

A Novel In Vitro Disease Model for Systemic Sclerosis Using Polyisocyanide Hydrogels

Microenvironment with NIR-Controlled ROS and Mechanical Tensions for Manipulating Cell Activities in Wound Healing

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