Polyionic hybrid nano-engineered systems comprising alginate and chitosan for antihypertensive therapeutics

Niaz, Taskeen; Nasir, Habib; Shabbir, Saima; Rehman, Asma; Imran, Muhammad

doi:10.1016/j.ijbiomac.2016.05.055

Cited by 24 publications

(11 citation statements)

References 41 publications

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“…One of the most utilized biopolymer is chitosan (CS) due to its beneficial chemical-physical properties [ 1 ]. Previous studies reported its antioxidant [ 2 ], anti-hypertensive [ 3 ], anti-diabetic, anti-obesity [ 4 ], neuroprotective [ 5 ] and bacteriostatic effects [ 6 ]. Moreover, chitosan plays an important role in the cell regulation and tissue regeneration [ 7 , 8 ].…”

Section: Introductionmentioning

confidence: 99%

Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery

et al. 2017

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This work investigated the preparation of chitosan nanoparticles used as carriers for doxorubicin for targeted cancer delivery. Prepared nanocarriers were stabilized and functionalized via zinc ions incorporated into the chitosan nanoparticle backbone. We took the advantage of high expression of sarcosine in the prostate cancer cells. The prostate cancer targeting was mediated by the AntiSar antibodies decorated surface of the nanocage. Formation of the chitosan nanoparticles was determined using a ninhydrin assay and differential pulse voltammetry. Obtained results showed the strong effect of tripolyphosphine on the nanoparticle formation. The zinc ions affected strong chitosan backbone coiling both in inner and outer chitosan nanoparticle structure. Zinc electrochemical signal depended on the level of the complex formation and the potential shift from −960 to −950 mV. Formed complex is suitable for doxorubicin delivery. It was observed the 20% entrapment efficiency of doxorubicin and strong dependence of drug release after 120 min in the blood environment. The functionality of the designed nanotransporter was proven. The purposed determination showed linear dependence in the concentration range of Anti-sarcosine IgG labeled gold nanoparticles from 0 to 1000 µg/mL and the regression equation was found to be y = 3.8x − 66.7 and R2 = 0.99. Performed ELISA confirmed the ability of Anti-sarcosine IgG labeled chitosan nanoparticles with loaded doxorubicin to bind to the sarcosine molecule. Observed hemolytic activity of the nanotransporter was 40%. Inhibition activity of our proposed nanotransporter was evaluated to be 0% on the experimental model of S. cerevisiae. Anti-sarcosine IgG labeled chitosan nanoparticles, with loaded doxorubicin stabilized by Zn ions, are a perspective type of nanocarrier for targeted drug therapy managed by specific interaction with sarcosine and metallothionein for prostate cancer.

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Section: Introductionmentioning

confidence: 99%

Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery

et al. 2017

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“…The combination of chitosan and alginate leads the spontaneous aggregation and formation of polyion complexes through the interaction of the carboxyl and amine groups within the structure of both polymers, respectively [22]. Additionally, the electrostatic interaction helps with the formation of polyelectrolyte complexes by using a crosslinking agent such as STPP, which contributes to the crosslinking and orientation of the nanoparticles during the growth [23][24][25]. Some reports have shown that the variation of several parameters such as the concentration and weight ratio of chitosan and alginate, as well as the pH solution and addition order, can significantly affect the size and shape of the final nanoparticle and even the structure that may be either heterogeneous or homogenous [22].…”

Section: Resultsmentioning

confidence: 99%

“…In Vitro Cytotoxicity Assessment. Trypan blue and Cell-Titer 96® AQueous tests were performed to evaluate cytotoxicity using different concentrations (10,25, and 50 μg/mL) of Cs-Al NDs against two cell lines, PWR-1E and PC-3. According to the results from both methods shown in Figures 6(a)-6(d) and 7(a)-7(d), no significant toxicity was observed in the cell lines providing a viability above 80%, which is in agreement with the cytotoxicity requirements for the application of biomaterials in drug delivery systems [50,51].…”

Section: Encapsulation Efficacy Andmentioning

confidence: 99%

Ionotropic Gelation Synthesis of Chitosan-Alginate Nanodisks for Delivery System and In Vitro Assessment of Prostate Cancer Cytotoxicity

Patiño-Ruiz

Marrugo

Reyes

et al. 2020

International Journal of Polymer Science

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We report on the synthesis of chitosan-alginate nanodisks (Cs-Al NDs) using a simple approach consisting of the ionotropic gelation method. Sodium tripolyphosphate (STPP) was used as crosslinking agent to promote the electrostatic interaction between amine groups the chitosan and hydroxyl and carboxyl groups of alginate. Scanning electron microscopy (SEM) images provided direct evidence of the morphology of the nanodisks where agglomeration was observed due to the electrostatic interaction between the functional groups. Furthermore, dynamic light scattering (DLS) showed that the hydrodynamic size of the Cs-Al NDs was 227 nm and 152 nm in pH 1.2 and pH 7.4, respectively, which is in agreement with the information observed in the SEM images. The chemical structure is presented mainly the amine and carboxyl groups due to the presence of chitosan and alginate in the nanodisks, respectively, which allow the electrostatic interaction through N-H linkages. According to the X-ray diffraction, we found that the Cs-Al NDs exhibited the typical structure of chitosan and alginate, which lead the formation of polyelectrolyte complexes. We also evaluated the encapsulation of amoxicillin in the nanodisk, obtaining a loading efficiency of 74.98%, as well as a maximum in vitro release amount of 63.2 and 52.3% at pH 1.2 and 7.4, respectively. Finally, the cytotoxicity effect of the Cs-Al nanodisks was performed in human prostatic epithelial PWR-1E and Caucasian prostate adenocarcinoma PC-3 cell lines, in which the cell viability was above 80% indicating low inhibition and determining the Cs-Al NDs as a promising technology for controlled delivery systems.

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“…These potential carriers are capable of delivering bioactive compounds to specific cells and tissues with minimal immune response at the nanoscale. Natural polymers, due to their diverse physico-chemical properties, can be used to fabricate polymeric nano-carrier systems (NCS) that can optimize the bioavailability of drugs by increasing retention time, minimizing side effects, increasing drug solubility, and reducing dosage frequency [4].…”

Section: Introductionmentioning

confidence: 99%

“…It consists of N-acetyl-d-glucosamine and d-glucosamine units. Considering the non-toxic, biocompatible, and biodegradable nature, chitosan is a prospective candidate in the field of pharmaceutical industry [4,5]. Several researchers have investigated chitosan in gene delivery, drug release, and biomedical areas [6][7][8][9].…”

Section: Introductionmentioning

confidence: 99%

A Smart Drug Delivery System Based on Biodegradable Chitosan/Poly(allylamine hydrochloride) Blend Films

et al. 2020

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The amalgamation of natural polysaccharides with synthetic polymers often produces fruitful results in the area of drug delivery due to their biodegradable and biocompatible nature. In this study, a series of blend films composed of chitosan (CS)/poly(allylamine hydrochloride) (PAH) in different compositions were prepared as smart drug delivery matrices. The properties of these polymeric films were then explored. Attenuated total reflectance-Fourier transform infrared (ATR-FTIR) analysis confirmed an intermolecular hydrogen bonding between CS and PAH. Atomic force microscopy (AFM) revealed improvements in surface morphology as the percentage of PAH in the blend films increased up to 60% (w/w). Water contact angle (WCA) ranged between 97° to 115°, exhibiting the hydrophobic nature of the films. Two films were selected, CTH-1 (90% CS and 10% PAH) and CTH-2 (80% CS and 20% PAH), to test for in vitro cumulative drug release (%) at 37 ± 0.5 °C as a function of time. It was revealed that for simulated gastric fluid (SGF) with pH 1.2, the cumulative drug release (CDR) for CTH-1 and CTH-2 was around 88% and 85% in 50 min, respectively. Both films converted into gel-like material after 30 min. On the other hand, in pH 7.4 phosphate buffer saline (PBS) solution, the maximum CDR for CTH-1 and CTH-2 was 93% in 90 min and 98% in 120 min, respectively. After 120 min, these films became fragments. Sustained drug release was observed in PBS, as compared to SGF, because of the poor stability of the films in the latter. These results demonstrate the excellent potential of blend films in sustained-release drug delivery systems for hydrophilic or unstable drugs.

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Polyionic hybrid nano-engineered systems comprising alginate and chitosan for antihypertensive therapeutics

Cited by 24 publications

References 41 publications

Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery

Zinc-Modified Nanotransporter of Doxorubicin for Targeted Prostate Cancer Delivery

Ionotropic Gelation Synthesis of Chitosan-Alginate Nanodisks for Delivery System and In Vitro Assessment of Prostate Cancer Cytotoxicity

A Smart Drug Delivery System Based on Biodegradable Chitosan/Poly(allylamine hydrochloride) Blend Films

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