Polyion complex stability and gene silencing efficiency with a siRNA-grafted polymer delivery system

Takemoto, Hiroyuki; Ishii, Atsushi; Miyata, Kanjiro; Nakanishi, Masataka; Oba, Makoto; Ishii, Takehiko; Yamasaki, Yuichi; Nishiyama, Nobuhiro; Kataoka, Kazunori

doi:10.1016/j.biomaterials.2010.07.015

Cited by 118 publications

(68 citation statements)

References 36 publications

(20 reference statements)

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“…In vivo transfection methods using recombinant viral vectors or nonviral drug delivery system (DDS) carriers, such as cationic polymers and liposomes, have been investigated widely [1][2][3][4] ; however, the methods that deliver naked nucleic acids using physical stimuli are considered the most straightforward because of low toxicities associated with the transfection agents, convenient preparation, ease of handling, and so on.…”

mentioning

confidence: 99%

Liver Suction-Mediated Transfection in Mice Using a Pressure-Controlled Computer System

Shimizu

Zhang

Kawakami

et al. 2014

Biological & Pharmaceutical Bulletin

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We previously developed an in vivo tissue suction-mediated transfection method (denoted as the tissue suction method) for naked nucleic acids, such as plasmid DNA (pDNA) and small interfering RNA (siRNA), in mice. However, it remains unclear whether the suction pressure conditions affect the results of this method. Therefore, in the present study, we assembled a computer system to control the suction pressure and investigate the effects of the suction pressure conditions on the efficiency of the liver suction transfection of naked pDNA that encodes luciferase in mice. Using the developed system, we examined the effects of the minimum magnitude of the suction pressure, suction pressure waveform, and suction times of the luciferase expression level in mice livers. We determined that the liver suction method at 5 kPa was not only effective but also caused the lowest hepatic toxicity in mice. Additionally, the results indicated that the suction pressure waveform affects the luciferase expression levels, and a single period of suction on the targeted portion of the liver is sufficient for transfection. Thus, the developed system is useful for performing the tissue suction method with high accuracy and safety.

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confidence: 99%

Liver Suction-Mediated Transfection in Mice Using a Pressure-Controlled Computer System

Shimizu

Zhang

Kawakami

et al. 2014

Biological & Pharmaceutical Bulletin

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“…On one hand, siRNA is well protected from degradation and non-specifi c clearance by PICs in the extracellular milieu, and on the other hand, owing to the selective release of siRNA in the intracellular milieu, higher transfection effi ciency is observed. Also, siRNA grafted to poly(aspartic acid) [PAsp(-SS-siRNA)] via a disulfi de linkage was found to give a higher siRNA effi ciency due to effi cient siRNA release from the PIC under intracellular reductive conditions (Takemoto et al 2010). …”

Section: Rna Interference Technology With Emphasis On Delivery Vehiclmentioning

confidence: 98%

RNA interference technology with emphasis on delivery vehicles—prospects and limitations

Prabha

Vyas

Gupta

et al. 2015

Artificial Cells, Nanomedicine, and Biotechnology

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“…However, there has been little success in developing PECs for oligonucleotide delivery because complexation typically results in formation of large and unstable polyplexes under physiological conditions. [54,55] Structural differences between pDNA and oligonucleotides, like flexibility ( e.g. , persistence length ssDNA l p ≈ .6 nm versus dsDNA l p ≈ 53 nm[56]) and charge density ( e.g.…”

Section: Bulk Polyelectrolyte Complexesmentioning

confidence: 99%

“…[57] Poor complexation stability has also been addressed via siRNA chemical modifications, including PEG-siRNA, sticky-siRNA, poly-siRNA and multimerized siRNA. [55] Takemoto et al developed an siRNA-grafted poly(aspartic acid) via disulfide linkages, which allows for cleavage under reductive environments. The increased charge number and density aided in stable polyplex formation with a poly(aspartamide) modified for endosomal escape.…”

Section: Bulk Polyelectrolyte Complexesmentioning

confidence: 99%

Bulk and nanoscale polypeptide based polyelectrolyte complexes

Marciel

Chung

Brettmann

et al. 2017

Advances in Colloid and Interface Science

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Polyelectrolyte complexes (PECs) formed using polypeptides have great potential for developing new self-assembled materials, in particular for the development of drug and gene delivery vehicles. This review discusses the latest advancements in PECs formed using polypeptides as the polyanion and/or the polycation in both polyelectrolyte complexes that form bulk materials and block copolymer complexes that form nanoscale assemblies such as PEC micelles and other self-assembled structures. We highlight the importance of secondary structure formation between homogeneous polypeptide complexes, which, unlike PECs formed using other polymers, introduces additional intermolecular interactions in the form of hydrogen bonding, which may influence precipitation over coacervation. However, we still include heterogeneous complexes consisting of polypeptides and other polymers such as nucleic acids, sugars, and other synthetic polyelectrolytes. Special attention is given to complexes formed using nucleic acids as polyanions and polypeptides as polycations and their potential for delivery applications.

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Polyion complex stability and gene silencing efficiency with a siRNA-grafted polymer delivery system

Cited by 118 publications

References 36 publications

Liver Suction-Mediated Transfection in Mice Using a Pressure-Controlled Computer System

Liver Suction-Mediated Transfection in Mice Using a Pressure-Controlled Computer System

RNA interference technology with emphasis on delivery vehicles—prospects and limitations

Bulk and nanoscale polypeptide based polyelectrolyte complexes

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