Polyion complex micelles formed from glucose oxidase and comb‐type polyelectrolyte with poly(ethylene glycol) grafts

Kawamura, Akifumi; Kojima, Chie; Iijima, Michihiro; Harada, Atsushi; Kono, Kenji

doi:10.1002/pola.22739

Cited by 16 publications

(20 citation statements)

References 28 publications

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“…PIC micelle combines with oppositely charged protein by electrostatic interactions under mild conditions. [35][36][37][38] The loaded protein can avoid rapid degradation in vivo. Glycyrrhetinic acid (GA) has been affirmed to possess affluent receptors with high affinity on hepatocyte membrane.…”

Section: Introductionmentioning

confidence: 99%

In vitro and in vivo protein release and anti-ischemia/<br />reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(L-lysine) nanoparticles

Fang¹,

Liu²,

Jiang³

et al. 2017

IJN

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Here, we describe a bone morphogenetic protein-2 (BMP-2) nanocarrier based on glycyrrhetinic acid (GA)-poly(ethylene glycol) (PEG)-b-poly( l -lysine) (PLL). A protein nanocarrier was synthesized, characterized and evaluated as a BMP-2 delivery system. The designed nanocarrier was synthesized based on the ring-opening polymerization of amino acid N-carboxyanhydride. The final product was measured with 1 H nuclear magnetic resonance. GA-PEG-b-PLL nanocarrier could combine with BMP-2 through electrostatic interaction to form polyion complex (PIC) micelles. BMP-2 could be rapidly and efficiently encapsulated through the GA-PEG-b-PLL nanocarrier under physiological conditions, exhibiting efficient encapsulation and sustained release. In addition, the GA-PEG-b-PLL-mediated BMP-2 delivery system could target the liver against hepatic diseases as it has GA-binding receptors. The anti-hepatic ischemia/reperfusion injury (anti-HI/RI) effect of BMP-2/GA-PEG-b-PLL PIC micelles was investigated in rats using free BMP-2 and BMP-2/PEG-b-PLL PIC micelles as controls, and the results showed that BMP-2/GA-PEG-b-PLL PIC micelles indicated significantly enhanced anti-HI/RI property compared to BMP-2 and BMP-2/PEG-b-PLL. All results suggested that GA-PEG-b-PLL could be used as a potential BMP-2 nanocarrier.

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Section: Introductionmentioning

confidence: 99%

In vitro and in vivo protein release and anti-ischemia/<br />reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(L-lysine) nanoparticles

Fang¹,

Liu²,

Jiang³

et al. 2017

IJN

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“…26 These graft copolymers are abbreviated as 2k13, 2k26, 5k12 and 5k21, respectively. The TiO 2 NP dispersion with a crystal structure of anatase (10 nm, pH < 3) was purchased from Ishihara Sangyo Kaisha, Ltd. Fluorescence probe molecules for ROS detection (dihydrorhodamine 123 (DHR-123) and singlet oxygen sensor green (SOSG)) were purchased from Lambda and Invitrogen, respectively.…”

Section: Methodsmentioning

confidence: 99%

Titanium dioxide nanoparticle-entrapped polyion complex micelles generate singlet oxygen in the cells by ultrasound irradiation for sonodynamic therapy

et al. 2013

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A new modality of using ultrasound irradiation instead of photoactivation, as in photodynamic therapy (PDT), sonodynamic therapy, has emerged as a promising treatment for various types of cancer. Titanium dioxide (TiO 2 ) has the ability to generate reactive oxygen species (ROS) by not only photo-but also ultrasound-irradiation. Here, the formation of core-shell type polyion complex micelles from TiO 2 nanoparticles with polyallylamine bearing poly(ethylene glycol) grafts effectively improves the dispersion stability of the TiO 2 nanoparticles under physiological conditions for therapeutic application. The TiO 2 nanoparticles in the micelles can generate ROS including singlet oxygen ( 1 O 2 ) by sonication. Furthermore, the micelles are taken up into HeLa cells and the TiO 2 nanoparticles generate 1 O 2 , which is widely believed to be the main cytotoxic agent in PDT, even in the cells treated by sonication. This is the first result representing 1 O 2 generation of TiO 2 nanoparticles in HeLa cells by sonication. Further, the micelles can selectively exhibit a cell-killing effect at only the ultrasound-irradiated area.

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“…As pointed out above, this point can be conveniently identified using the correlation between the polydispersity obtained from scattering studies and the molar ratio of polymer and protein. [33] Harada et al [16] investigated the PIC micelles in more detail using scattering techniques. At an equimolar ratio of both charges, the PIC micelle with a hydrodynamic diameter of D h ¼ 47 nm was estimated to have 42 polymer chains and 36 lysozymes.…”

Section: Binding Proteins Using Charged Polymersmentioning

confidence: 99%

“…For example, PIC micelles based on the much larger glucose oxidase (GOx) led to slightly smaller sizes at higher polymer content. [33] 0…”

Section: Binding Proteins Using Charged Polymersmentioning

confidence: 99%

“…[43] The influence of the length of copolymers with grafted PEG chain on the formation of PIC micelles with GOx (160 kDa) was investigated. [33] Although the molecular mass of PEG-graftpoly(allyl amine) was varied between 31 and 258 kDa, the radius of gyration of the resulting PIC micelle with GOx increased from 10.8 to 12.6 nm. Detailed studies revealed that PIC micelles made from longer polymers contained fewer enzyme molecules.…”

Section: Both Charges In Balancementioning

confidence: 99%

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Polyion Complex Micelles for Protein Delivery

Chen¹,

Stenzel²

2018

Aust. J. Chem.

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Proteins are ubiquitous in life and next to water, they are the most abundant compounds found in human bodies. Proteins have very specific roles in the body and depending on their function, they are for example classified as enzymes, antibodies or transport proteins. Recently, therapeutic proteins have made an impact in the drug market. However, some proteins can be subject to quick hydrolytic degradation or denaturation depending on the environment and therefore require a protective layer. A range of strategies are available to encapsulate and deliver proteins, but techniques based on polyelectrolyte complex formation stand out owing to their ease of formulation. Depending on their isoelectric point, proteins are charged and can condense with oppositely charged polymers. Using block copolymers with a neutral block and a charged block results in the formation of polyion complex (PIC) micelles when mixed with the oppositely charged protein. The neutral block stabilises the charged protein–polymer core, leading to nanoparticles. The types of micelles are also known under the names interpolyelectrolyte complex, complex coacervate core micelles, and block ionomer complexes. In this article, we discuss the formation of PIC micelles and their stability. Strategies to enhance the stability such as supercharging the protein or crosslinking the PIC micelles are discussed.

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Polyion complex micelles formed from glucose oxidase and comb‐type polyelectrolyte with poly(ethylene glycol) grafts

Cited by 16 publications

References 28 publications

In vitro and in vivo protein release and anti-ischemia/<br />reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(L-lysine) nanoparticles

In vitro and in vivo protein release and anti-ischemia/<br />reperfusion injury properties of bone morphogenetic protein-2-loaded glycyrrhetinic acid-poly(ethylene glycol)-b-poly(L-lysine) nanoparticles

Titanium dioxide nanoparticle-entrapped polyion complex micelles generate singlet oxygen in the cells by ultrasound irradiation for sonodynamic therapy

Polyion Complex Micelles for Protein Delivery

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