Polyhydroxyfullerene Binds Cadmium Ions and Alleviates Metal-Induced Oxidative Stress in Saccharomyces cerevisiae

Pradhan, Arunava; Pinheiro, José Paulo; Seena, Sahadevan; Pascoal, Cláudia; Cássio, Fernanda

doi:10.1128/aem.01329-14

Cited by 12 publications

(3 citation statements)

References 53 publications

(79 reference statements)

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“…Special attention has been given to fullerenols with a large number of hydroxyl groups ( n > 16), since they are nontoxic for the most of examined biological model systems, possess significant antioxidative properties [ 13 ], and produce radical oxygen species, such as singlet oxygen and superoxide, after UV photosensitization [ 14 ]. Experiments conducted on various aquatic organisms and yeasts have proven that fullerenol nanoparticles (FNP) stimulate growth and exhibit protective effects from heavy metal induced toxicity [ 15 , 16 ].…”

Section: Introductionmentioning

confidence: 99%

Aspergilli Response to Benzalkonium Chloride and Novel‐Synthesized Fullerenol/Benzalkonium Chloride Nanocomposite

Unković

Grbić

Stupar

et al. 2015

The Scientific World Journal

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A comprehensive comparative analysis of antifungal potential of benzalkonium chloride and newly synthesized fullerenol/benzalkonium chloride nanocomposite was conducted to assess the possible impact of carbon-based nanocarrier on antimicrobial properties of the commonly used biocide. Physical characterization of synthesized nanocomposite showed zeta potential of +37.4 mV and inhomogeneous particles size distribution, with nanocomposite particles' dimensions within 30–143 nm and maximum number of particles at 44 nm. The effect of pure and fullerenol nanocarrier-bound biocide was evaluated in eight Aspergillus species. In mycelial growth assay, nanocomposite was more potent, as fungicidal effect of 1.04/0.6 μg mL−1 was obtained in all but one of the isolates (A. niger), while proportional concentration of pure biocide (0.6 μg mL−1) completely inhibited mycelial growth of only three Aspergillus species. However, conidia appear to be less susceptible to nanocomposite treatment, as lower fungistatic (MIC) and fungicidal (MFC) concentrations were obtained with biocide alone (MIC in range from 0.03 to 0.15 μg mL−1 and MFC from 0.075 to 0.45 μg mL−1). To a different degree, both substances stimulated aflatoxin B1 production and inhibited ochratoxin A synthesis. Very low mycelium biomass yield, in range from 1.0 to 3.0 mg dry weight, was documented in both biocide and nanocomposite enriched medium.

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Section: Introductionmentioning

confidence: 99%

Aspergilli Response to Benzalkonium Chloride and Novel‐Synthesized Fullerenol/Benzalkonium Chloride Nanocomposite

Unković

Grbić

Stupar

et al. 2015

The Scientific World Journal

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“…published in Current Opinion 3 (2017) 144-162Saccharomyces cerevisiae in presence and absence of polyhydroxyfullerenes[98],…”

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confidence: 99%

A review on electrochemical methods for trace metal speciation in environmental media

Companys

Galceran

Pinheiro

et al. 2017

Current Opinion in Electrochemistry

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11Trace metal speciation is key to understand/predict bioavailability and potential toxicity 12 of metals to biota and will, undoubtedly, be incorporated in future regulations. 13Electroanalytical methods have a role to play in such development: they offer a wide 14 range of advantages such as speed, portability, economy, solid interpretation 15 backgrounds and low limits of quantification. This review focusses on three selected 16 stripping techniques: Competitive-Ligand Exchange -Cathodic Stripping Voltammetry 17 (CLE-CSV), Scanned Stripping Chrono-Potentiometry (SSCP) and AGNES (Absence 18 of Gradients and Nernstian Equilibrium Stripping) reporting their working principles, 19 characteristics (strong and weak points) and recent applications to systems of 20 environmental relevance (such as seawaters, freshwaters or soil extracts). 21 22 23 24 Keywords: speciation, availability, electroanalysis, electrochemistry, heavy metal, 25 CLE-CSV, SSCP, AGNES 26 27 published in Current Opinion 3 (2017) 144-162 33 Speciation (i.e. the distribution of an element among its different chemical forms[1]) 34 has been recognised as a key factor for trace metal (bio)availability to biota, as 35 postulated by hegemonic ecotoxicological paradigms such as the Free Ion Activity 36 Model (FIAM) [2] or the Biotic Ligand Model (BLM)[3], both assuming that the free 37 ion is the only bioavailable species. The limits of these models have recently been 38 reviewed [4], highlighting numerous cases where specific fractions of metal complexes 39 can be internalized, either directly or through the dissociation of labile complexes. Thus, 40there is a need to design, develop, apply and interpret analytical techniques able to 41 provide reliable speciation information [5][6][7] that can be used by regulatory bodies. 42Although there are numerous non-electrochemical techniques [5][6][7][8][9][10][11][12][13], those that are 43 provide attractive possibilities such as speed of analysis, low-cost and miniaturization in 44 view to on-site (and in-situ) measurements [14], and can also provide thermodynamic 45 (i.e. equilibrium) information (such as the amount of the free metal, the amount of 46 complexing sites, the stoichiometry of complexes and their stability constants [15]) as 47 [16;17]. Stripping techniques, due to their low detection limits, are well suited for 50 environmental analysis. They are two-stage techniques, where the first stage is a 51 deposition step that accumulates the analyte (at the electrode surface or in its volume), 52 followed by a second stage that quantifies this analyte. Anodic Stripping Voltammetry 53 (ASV), in its multiple variants, is the most popular and has the advantage to possibly be 54 measuring a potentially bioavailable fraction of the metal, as shown again recently 55 [18;19]. 56 57 published in Current Opinion 3 (2017) 144-162 This critical evaluation focusses on three techniques that are known powerful tools for 58 metal speciation: AGNES (Absence of Gradients and Nernstian Equilibrium Stripping) 59 and SSCP...

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“…Protection of protein and lipid was one of the possible mechanisms that sucrose preincubation can improve the yeast's cadmium resistance. Pradhan et al reported that water‐soluble polyhydroxy fullerene could decrease cadmium‐induced oxidative stress and recover the S . cerevisiae growth, which was suppressed by the presence of cadmium.…”

Section: Discussionmentioning

confidence: 99%

Improved cadmium resistance and removal capacity in Pichia kudriavzevii A16 by sucrose preincubation

Zhang

Guo

et al. 2019

J Basic Microbiol

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Removal of heavy metals from food material by growing micro-organisms is limited by the toxicity to cells. In this study, different preincubation treatments were investigated to analyze their effects on cadmium resistance and removal ability of Pichia kudriavzevii A16 and Saccharomyces cerevisiae CICC1211. Sucrose preincubation improved the cadmium resistance of both yeast cells and increased the cadmium-removal rate of P. kudriavzevii A16. An evident decrease of intracellular and cell-surface cadmium accumulation was observed after sucrose preincubation, which may be the primary reason responsible for the improved cadmium resistance. Flow cytometry assay showed that sucrose significantly reduced the production of reactive oxygen species (ROS) and cell death rate of both yeasts under cadmium compared with those normally cultured cells. Under cadmium stress, the content of both protein carbonyls and malonyldialdehyde were also reduced by the addition of sucrose, the results were in accordance with the tendency of ROS, exhibiting a defending function of sucrose. Osmotic regulators as proline and trehalose were increased by sucrose preincubation in P. kudriavzevii A16 in the presence of cadmium. The results suggested that sucrose preincubation could be applied to improve cadmium resistance and removal rate of yeasts. K E Y W O R D Scadmium removal, cadmium resistance, cross-protection, Pichia kudriavzevii, sucrose preincubation

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Polyhydroxyfullerene Binds Cadmium Ions and Alleviates Metal-Induced Oxidative Stress in Saccharomyces cerevisiae

Cited by 12 publications

References 53 publications

Aspergilli Response to Benzalkonium Chloride and Novel‐Synthesized Fullerenol/Benzalkonium Chloride Nanocomposite

Aspergilli Response to Benzalkonium Chloride and Novel‐Synthesized Fullerenol/Benzalkonium Chloride Nanocomposite

A review on electrochemical methods for trace metal speciation in environmental media

Improved cadmium resistance and removal capacity in Pichia kudriavzevii A16 by sucrose preincubation

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