Polyhydroxyalkanoate Production from Two Species of Marine Bacteria: A Comparative Study

Blandón, Lina M.; Alvarado-Campo, Katleen L.; Patiño, Albert D.; Jiménez-Vergara, E.; Quintero, Marynes; Montoya-Giraldo, Manuela; Jutinico-Shubach, Laura Marcela; Santos-Acevedo, Marisol; Gomez‐León, Javier

doi:10.1007/s10924-020-01770-3

Cited by 5 publications

(2 citation statements)

References 42 publications

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“…The determination of sugar consumption was estimated by Di-Nitrosalicylic acid (DNS method) throughout each fermentation period according to the method described by Miller [Blandón et al, 2020]. This method gives a rapid estimation by measuring the total amount of reducing sugars in the hydrolysate.…”

Section: Dns Methods (Sugar Consumption)mentioning

confidence: 99%

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

Yasin¹,

Al-Mayaly²

2021

J. Ecol. Eng.

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Polyhydroxyalkanoates (PHAs) have gained much attention as biodegradable polymers, many efforts are being made to minimize the cost of PHAs by finding cheap carbon source depending on the type of microorganism and fermentation conditions. The aims of this study were to evaluate the effects of different glucose concentrations and other important conditions on the PHA production by Bacillus cereus isolated from soil. Polyhydroxyalkanoates PHAs accumulated by soil microorganisms were examined by screening the isolated bacteria using Sudan B Black and Nile Blue staining process. A Gram positive strain was identified using the 16s rRNA gene, deposited in the NCBI GenBank sequence database. Different growth conditions (favorite glucose concentrations 1-8 % (w/v), temperatures and pH) were tested and the growth parameters (sugar consumption, cell counting and Cell Dry Weight CDW) were studied. The extracted polymers were analyzed and characterized using an FTIR spectrophotometer followed by a GC-MS analysis. The pure bacterial strain isolated from soil was deposited in the NCBI GenBank database B. cereus strain ARY73, which showed significant black colored granules (or dark blue) using Sudan B Black stain, it also showed positive to Nile blue A as a high indicator stain for PHA accumulation. B. cereus ARY73 showed high production of PHA using (w/v): 2% glucose and 1% nitrogen source at 35 °C and pH7 yields 79% per Cell Dry Weight and 96 h of incubation. The extracted polymers were analyzed and characterized using an FTIR spectrophotometer confirming the PHA structure. The FTIR spectrophotometer, followed by a GC-MS analysis indicated the Scl-co-mcl PHA structure. This research demonstrates that the isolated strain B. cereus ARY73 was a good candidate for PHA production with a better quality for use in biomedical and other applications. The use of biopolymer in soil, enhanced the accumulation of the microorganisms (such as bacteria) capable of degrading biopolymer or biodegradation by-products yields by other species which were isolated in this study and demonstrated their efficiency in producing biopolymers.

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Section: Dns Methods (Sugar Consumption)mentioning

confidence: 99%

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

Yasin¹,

Al-Mayaly²

2021

J. Ecol. Eng.

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“…In Colombia, microbial bioprospection has been mainly focused on terrestrial environments, whereas those organisms that inhabit marine environment have been little explored, and particularly the extremophiles found in fumaroles, hot springs, coastal salt lagoons and deep sea pits are of interest (Gómez et al, 2000). Recent reports have focused on bacteria isolated from marine sediments, to detect antimicrobial activity (Quintero et al, 2018), biofilm formation (López et al, 2019), biosurfactant production (Santos‐Acevedo et al, 2018), polyhydroxyalkanoate production (Blandón et al, 2020) and hydrocarbon degradation potential (Narváez‐Flórez et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

In vitro biological activity of extracts from marine bacteria cultures against Toxoplasma gondii and Mycobacterium tuberculosis

Quintero

Blandón

Vidal

et al. 2022

Journal of Applied Microbiology

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Aims:To evaluate the biological activity of extracts from cultures of marine bacteria against Toxoplasma gondii and Mycobacterium tuberculosis. Methods and Results: Ethyl acetate extracts obtained from seven marine bacteria were tested against T. gondii GFP-RH and M. tuberculosis H37Rv. The cytotoxicity on HFF-1 cells was measured by a microplate resazurin fluorescent approach, and the haemolytic activity was determined photometrically. The extracts from Bacillus sp. (INV FIR35 and INV FIR48) affected the tachyzoite viability. The extracts from Bacillus, Pseudoalteromonas, Streptomyces and Micromonospora exhibited effects on infection and proliferation processes of parasite. Bacillus sp. INV FIR48 extract showed an minimum inhibitory concentration value of 50 µg ml −1 against M. tuberculosis H37Rv. All the extracts exhibited relatively low toxicity to HFF-1 cells and the primary culture of erythrocytes, except Bacillus sp. INV FIR35, which decreased cell viability under 20%. Liquid chromatography coupled to mass spectrometry analysis of the most active bacterial extract Bacillus sp. INV FIR48 showed the presence of peptide metabolites related to surfactin. Conclusions: The extract from culture of deep-sea Bacillus sp. INV FIR48 showed anti-T. gondii and anti-tuberculosis (TB) biological activity with low cytotoxicity. In addition, peptide metabolites were detected in the extract. Significance and Impact of the Study: Toxoplasmosis and TB are among the most prevalent diseases worldwide, and the current treatment drugs exhibit side effects.This study confirm that marine bacteria are on hand sources of anti-infective natural products.

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Marine bacteria associated with the green seaweed Ulva sp. for the production of polyhydroxyalkanoates

Gnaim

Polikovsky

Unis

et al. 2021

Bioresource Technology

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The biosynthesis of polyhydroxyalkanoate (PHA) biopolymers from certain marine microbes, associated with green macroalgae Ulva sp., has attracted significant attention. The Ulva sp. is abundant biomass in numerous locations around the world and could be easily cultivated by marine farming. The variety of sugars found in Ulva sp. homogenate could be used as a carbon source for microbial growth and PHA production. In this work, we isolated and explored a series of bacterial strains that function as potential producers of P(3HB), utilizing a range of common sugars found in Ulva sp. Analysis of 16S rDNA gene-sequence revealed that the PHA-producing bacteria were phylogenetically related to species of the genus Cobetia, Bacillus, Pseudoaltermonas, and Sulfitobacter. The highest-yield of P(3HB) was observed in the case of new Cobetia strain, C. amphilecti, with up to 61% (w/w) in the presence of mannitol and 12% (w/w) on Ulva sp. acid hydrolysate as a substrate.

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Polyhydroxyalkanoate Production from Two Species of Marine Bacteria: A Comparative Study

Cited by 5 publications

References 42 publications

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

Study of The Fermentation Conditions of the Bacillus Cereus Strain ARY73 to Produce Polyhydroxyalkanoate from Glucose

In vitro biological activity of extracts from marine bacteria cultures against Toxoplasma gondii and Mycobacterium tuberculosis

Marine bacteria associated with the green seaweed Ulva sp. for the production of polyhydroxyalkanoates

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