Polyhydroxyalkanoate production by a novel bacterium Massilia sp. UMI-21 isolated from seaweed, and molecular cloning of its polyhydroxyalkanoate synthase gene

Han, Xuerong; Satoh, Yasuharu; Kuriki, Yumi; Seino, Teruyuki; Fujita, Shinji; Suda, T.; Kobayashi, Takanori; Tajima, Kenji

doi:10.1016/j.jbiosc.2014.04.022

Cited by 28 publications

(12 citation statements)

References 33 publications

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“…It has been recently described that a strain of Massilia sp. produced PHA (45% of CDM) from Ulva sp., green algae containing starch …”

Section: Bacterial Strains Producing Phas Can Be Modified To Use Diffmentioning

confidence: 99%

“…produced PHA (45% of CDM) from Ulva sp., green algae containing starch. 90 The use of starch as feedstock for PHA normally requires hydrolysis pre-treatments to produce glucose, 84 or acidogenesis of starchy substrates to make VFAs. 108 Bacteria and archaea have been reported for their ability to produce PHA also from starch hydrolysates.…”

Section: Starchy Residuesmentioning

confidence: 99%

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Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Favaro

Basaglia

Casella

2018

Biofuels Bioprod Bioref

132

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Polyhydroxyalkanoates (PHAs) are a family of biodegradable intracellular polyesters that a number of Eubacteria and Archaea can accumulate for energy and carbon storage. Most of the genetic modifications to the producing bacterial species have been accomplished to clarify basic biochemical, genetic, and metabolic aspects of PHA metabolism. However, due to its plastic‐like properties and complete biodegradability, this bio‐based polymer has attracted the attention of a variety of manufacturers. A number of genetic approaches have therefore been reported, aimed at improving the performance of the microorganisms with a potential for use in a production process. Indeed, genetic tools may find useful applications in all the phases of the PHA production chain, from the isolation and characterization of new microbial strains through all the production steps until they reach the downstream processes. The substrates generally used for PHA production are expensive, so the search for low‐cost feedstock is necessary. These materials, possibly deriving from agri‐food processes, are unfortunately not easily degraded or converted directly into PHAs. Thus, the development of engineered microbes is in progress to process waste streams and covert them to valuable polymers. This review will summarize the most relevant results obtained through genetic engineering tools for the production of PHAs from cheap carbon sources in view of possible industrial applications. © 2018 Society of Chemical Industry and John Wiley & Sons, Ltd.

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“…It has been recently described that a strain of Massilia sp. produced PHA (45% of CDM) from Ulva sp., green algae containing starch …”

Section: Bacterial Strains Producing Phas Can Be Modified To Use Diffmentioning

confidence: 99%

Section: Starchy Residuesmentioning

confidence: 99%

Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Favaro

Basaglia

Casella

2018

Biofuels Bioprod Bioref

132

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“…For example, Massilia was the common dominant genus in all the mosses detected based on rRNA libraries, while Flavobacterium and Chryseobacterium were abundant in both mosses of Entodon compressus and Hygroamblystegium noterophilum . As reported, Massilia is often isolated from some extreme environments and can produce polyhydroxyalkanoate ; while Flavobacterium spp. often isolated from water or plants shows resistance to multiple antibiotics such as rifampicin and streptomycin .…”

Section: Discussionmentioning

confidence: 89%

Comparative analysis of bacteria associated with different mosses by 16S rRNA and 16S rDNA sequencing

Tian

2016

J. Basic Microbiol.

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To understand the differences of the bacteria associated with different mosses, a phylogenetic study of bacterial communities in three mosses was carried out based on 16S rDNA and 16S rRNA sequencing. The mosses used were Hygroamblystegium noterophilum, Entodon compressus and Grimmia montana, representing hygrophyte, shady plant and xerophyte, respectively. In total, the operational taxonomic units (OTUs), richness and diversity were different regardless of the moss species and the library level. All the examined 1183 clones were assigned to 248 OTUs, 56 genera were assigned in rDNA libraries and 23 genera were determined at the rRNA level. Proteobacteria and Bacteroidetes were considered as the most dominant phyla in all the libraries, whereas abundant Actinobacteria and Acidobacteria were detected in the rDNA library of Entodon compressus and approximately 24.7% clones were assigned to Candidate division TM7 in Grimmia montana at rRNA level. The heatmap showed the bacterial profiles derived from rRNA and rDNA were partly overlapping. However, the principle component analysis of all the profiles derived from rDNA showed sharper differences between the different mosses than that of rRNA-based profiles. This suggests that the metabolically active bacterial compositions in different mosses were more phylogenetically similar and the differences of the bacteria associated with different mosses were mainly detected at the rDNA level. Obtained results clearly demonstrate that combination of 16S rDNA and 16S rRNA sequencing is preferred approach to have a good understanding on the constitution of the microbial communities in mosses.

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“…strain UMI-21 and Burkholderia sp. strain AIU M5M02 isolated from seaweeds were used for the production of PHB using starch and mannitol as carbon sources, respectively (28,29).…”

Section: Introductionmentioning

confidence: 99%

Production of poly(3-hydroyxybutylate) by a novel alginolytic bacterium Hydrogenophaga sp. strain UMI-18 using alginate as a sole carbon source

Yamaguchi

Narsico

Kobayashi³

et al. 2019

Journal of Bioscience and Bioengineering

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A novel alginolytic bacterium Hydrogenophaga sp. strain UMI-18 that produces poly (3-hydroyxybutylate) (PHB) in the alginate-mineral salt (AMS) medium containing 1% (w/v) sodium alginate as a sole carbon source was isolated from a decayed brown seaweed litter. The yield of PHB produced by strain UMI-18 was 1.1 ± 0.15 g/L of AMS and the PHB content in dried cell pellet was 58 ± 4% (w/w). Glucose, fructose, galactose, mannose, mannitol, sucrose and lactose were also available for the production of PHB by strain UMI-18. The yield of PHB in 1% (w/v) carbohydrate media reached 2.03~2.24 g/L for glucose and fructose, 0.75~1.64 g/L for alginate, galactose, mannitol and sucrose, and ~0.15 g/L for lactose. The PHB produced by strain UMI-18 showed a glass-transition temperature (Tg) at 4 °C , a melting temperature at 175 °C , and an average molecular mass of 860 kDa. Draft genome analysis of the strain UMI-18 revealed that an alginateassimilating gene cluster is located in contig 8 comprising 453,520 bp and the PHBsynthesis gene cluster is in contig 15 comprising 653,793 bp.

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Polyhydroxyalkanoate production by a novel bacterium Massilia sp. UMI-21 isolated from seaweed, and molecular cloning of its polyhydroxyalkanoate synthase gene

Cited by 28 publications

References 33 publications

Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Improving polyhydroxyalkanoate production from inexpensive carbon sources by genetic approaches: a review

Comparative analysis of bacteria associated with different mosses by 16S rRNA and 16S rDNA sequencing

Production of poly(3-hydroyxybutylate) by a novel alginolytic bacterium Hydrogenophaga sp. strain UMI-18 using alginate as a sole carbon source

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