Polygonumins A, a newly isolated compound from the stem of Polygonum minus Huds with potential medicinal activities

Abstract

Polygonumins A, a new compound, was isolated from the stem of Polygonum minus. Based on NMR results, the compound’s structure is identical to that of vanicoside A, comprising four phenylpropanoid ester units and a sucrose unit. The structure differences were located at C-3″″′. The cytotoxic activity of polygonumins A was evaluated on several cancer cell lines by a cell viability assay using tetrazolium dye 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT). The compound showed the highest antiproliferative (p < 0.05) activities against K562 (Human Leukaemia Cell Line), MCF7 (Human breast adenocarcinoma cell line), and HCT116 (Colorectal cancer cells) cells. Cytotoxic studies against V79–4 cells were carried out and showed that polygonumins A was toxic at 50 µg/ml, suggesting that this compound may be used as an anticancer drug without affecting normal cells. Polygonumins A also showed promising activity as an HIV-1 protease inhibitor with 56% relative inhibition. Molecular docking results indicated that the compound possesses high binding affinity towards the HIV protease over the low binding free energy range of -10.5 to -11.3 kcal/mol. P. minus is used in Malaysian traditional medicine for the treatment of tumour cells. This is the first report on the use of P. minus as an HIV-1 protease inhibitor.

HIV-1 protease and reverse transcriptase inhibition by tiger milk mushroom (Lignosus rhinocerus) sclerotium extracts: In vitro and in silico studies

“…The extracts (1 mg/mL, dissolved in 1% DMSO) were screened for HIV-1 PR inhibitory activity using an HIV-1 protease inhibitor screening kit (Fluorometric) (Biovision; Milpitas, CA, USA) according to the manufacturer's instruction as previously described. 12 Briefly, each sample was incubated with the HIV-1 PR enzyme at room temperature for 15 min. Then, the fluorescent substrate was added and the fluorescence (excitation/emission = 330/450 nm) was measured in a kinetic mode for 90 min at 37 °C using PerkinElmer EnSpire plate reader.…”

Potensi Antibakteri Ekstrak Metanol dan Senyawa Aromatik dari Buah Wualae (Etlingera elatior)

“…Kajian aspek kimia dan farmasi tanaman obat tradisional masyarakat Sulawesi Tenggara terus menerus dilakukan di Fakultas Farmasi Universitas Halu Oleo. Beberapa tanaman yang telah dipelajari antara lain Dipterocarpaceae (Juliawaty et al, 2009;Sahidin et al, 2017), Euphorbiaceae (Sahidin et al, 2011;Sahidin et al, 2013;Sabandar et al, 2013), Fabaceae (Al Muqarrabun et al, 2013), Poaceae (Ruslin et al, 2013), Polagonaceae (Sahidin et al, 2014;2015;Megantara et al, 2018;Ahmad et al, 2018), anacardiaceae (Wahyuni et al, 2018) dan Zingiberaceae (Sahidin et al, 2018).…”

Different Polarity Extracts of Polygonum minus towards Cytotoxic Activities against Colon Cancer Cell Lines (HT-29, HCT-116, CT-26)

“…minus is a tropical herb species from the Polygonaceae family that is commonly known as 'Kesum', 'Daun Laksa' or 'Cenohom' at Malaysia [5]. The plant is native to Southeast Asian nations such as Thailand, Malaysia, Indonesia, and Vietnam [6]. This herb is often used as a spice and flavouring enhancer in various dishes [7].…”