Polygonum tinctorium leaves suppress sodium dextran sulfate-induced colitis through interleukin-10-related pathway

Asari, Taka; Kikuchi, Hidezumi; Kawaguchi, Shogo; Sakuraba, Hirotake; Yoshida, Shukuko; Akemoto, Yui; Maeda, Takato; Ota, Satoshi; Murai, Yasuhisa; Higuchi, Naoko; Hoshi, Kentaro; Fukutoku, Yukari; Hiraga, Hiroto; Sasaki, Kenroh; Fukuda, Shinsaku

doi:10.1016/j.bbrep.2022.101272

Cited by 3 publications

(5 citation statements)

References 26 publications

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“…Some beneficial effects of indigo leaves may be unrelated to AhR signaling activation. Previously, we reported that P. tinctorium leaves improved murine colitis independently of AhR ( 14 ). 3,5,4'-trihydroxy-6,7-methylenedioxyflavone- O -glycosides and their aglycones, which are present in P. tinctorium , have been reported to suppress experimental colitis by inducing IL-10( 28 ).…”

Section: Discussionmentioning

confidence: 98%

“…Indirubin and its analogues exert anti-tumor effects by inhibiting cyclin-dependent kinases ( 13 ). It was recently reported that P. tinctorium leaves exerted anti-inflammatory effects by modulating the interleukin (IL)-10-related pathway in a mouse model of chemically induced colitis ( 14 ). However, the effectiveness of indigo leaf extract in HFD-induced intestinal damage has not yet been fully elucidated.…”

Section: Introductionmentioning

confidence: 99%

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Polygonum tinctorium leaf extract ameliorates high-fat diet-induced intestinal epithelial damage in mice

et al. 2023

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Dietary fat strongly influences the intestinal mucosal barrier, which protects against invading pathogenic bacteria. A high-fat diet (HFD) compromises the integrity of epithelial tight junctions (TJs) and reduces mucin production, leading to intestinal barrier disruption and metabolic endotoxemia. It has been shown that the active constituents of indigo plants can protect against intestinal inflammation; however, their protective role in HFD-induced intestinal epithelial damage remains unknown. The present study aimed to investigate the effects of Polygonum tinctorium leaf extract (indigo Ex) on HFD-induced intestinal damage in mice. Male C57BL6/J mice were fed a HFD and injected intraperitoneally with either indigo Ex or phosphate-buffered saline (PBS) for 4 weeks. The expression levels of TJ proteins, zonula occludens-1 and Claudin-1, were analyzed by immunofluorescence staining and western blotting. The colon mRNA expression levels of tumor necrosis factor-α, interleukin (IL)-12p40, IL-10 and IL-22 were measured by reverse transcription-quantitative PCR. The results revealed that indigo Ex administration attenuated the HFD-induced shortening of the colon. Colon crypt length was shown to be significantly greater in the indigo Ex-treated group mice compared with that in the PBS-treated group mice. Moreover, indigo Ex administration increased the number of goblet cells, and ameliorated the redistribution of TJ proteins. Notably, indigo Ex significantly increased the colon mRNA expression levels of IL-10. Indigo Ex displayed little effect on the gut microbial composition of HFD-fed mice. Taken together, these results suggested that indigo Ex may protect against HFD-induced epithelial damage. The leaves of indigo plants contain promising natural therapeutic compounds that could be used to treat obesity-associated intestinal damage and metabolic inflammation.

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Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Polygonum tinctorium leaf extract ameliorates high-fat diet-induced intestinal epithelial damage in mice

et al. 2023

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“…In this case, a significant suppression in TNF-α and a significant increase in IL-10 were observed in the DSS + Trp group compared to the DSS group ( Figure 3 c,d). As IL-10 is reported to be produced by Tregs [ 12 ], the reason for the increase in IL-10 in the DSS + Treg group may have been the induction of Tregs by Trp. It has also been reported that compounds that modulate the metabolism of Trp by the gut microbiota induced IL-10 and suppressed inflammation in a mouse colitis model [ 35 ].…”

Section: Discussionmentioning

confidence: 99%

“…In recent studies on fields of inflammatory diseases, including cancer and autoimmune diseases, the AhR has been reported to act as an important molecule that regulates the differentiation of Tregs, which express CD25 and the transcription factor, forkhead box P3 (Foxp3) [ 10 , 11 ]. Furthermore, recent studies have shown that AhR activation induces production of an anti-inflammatory cytokine, IL-10 [ 12 ], and this has attracted attention as a novel therapeutic target for IBD [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Immunoregulatory Effects of Elemental Diet and Its Ingredient, Tryptophan, via Activation of the Aryl Hydrocarbon Receptor in Mice

Kubota,

Imai,

Aoyagi

et al. 2024

IJMS

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Inflammatory bowel disease (IBD) is characterized by chronic intestinal inflammation and its treatment varies widely; however, when inflammation is high, a complete nutrient containing pre-digested elemental diet (ED) is used to preserve the intestinal tract. In this study, we investigated the mechanisms underlying the effectiveness of EDs for IBD using mice. C57BL/6 mice were orally treated with the ED (5 mL/day) and its ingredient L-tryptophan (Trp) (1–100 mg/kg), respectively. Flow cytometry analysis revealed that treatment with the ED and Trp (10 and 100 mg/kg) significantly increased the percentage of splenic CD4+-/CD25+-/Foxp3+ regulatory T cells (Tregs). In the 2% DSS-induced colitis-mouse model, Trp administration (100 mg/kg) led to a significant decrease in TNF-α and increase in IL-10 in the serum as well as a significant decrease in the inflammation score. Furthermore, the aryl hydrocarbon receptor (AhR) agonistic activity, which is a key function of Treg induction, of Trp and 15 Trp metabolites was characterized using a highly sensitive DR-EcoScreen cell assay. Five Trp metabolites, including L-kynurenine, acted as AhR agonists, while Trp did not. Taken together, these results suggest that the ED treatment has a Trp-dependent immunoregulatory effect, and several Trp metabolites that activate the AhR might contribute to induction of remission in patients with IBD.

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“…For this injury, many drugs were tested as treatment, including curcumin [ 84 ], garlic oil (which has antioxidant, anti-inflammatory and immunomodulatory effects) [ 85 ], carvacrol (a phenolic monoterpene extracted from Oreganum vulgarea sp. essential oils with antioxidant, anti-inflammatory and anticancer properties) [ 86 ], resveratrol [ 87 ], glucose-lysine Maillard reaction products [ 88 ], liquorice (a Glycyrrhiza uralensis rhizome-derived product with anti-inflammatory activity) [ 89 ], Lactobacillus sakei K040706 (with immuno-stimulatory effects) [ 90 ] and Polygonum tinctorium leaves extract (by enhancing the mRNA expression of interleukin-10 and decreasing expression of tumour necrosis factor in colon tissues) [ 91 ].…”

Section: Biomedical Applications Of Modified Dexmentioning

confidence: 99%

Dextran Formulations as Effective Delivery Systems of Therapeutic Agents

Petrovici¹,

Pinteala²,

Simiónescu

2023

Molecules

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Dextran is by far one of the most interesting non-toxic, bio-compatible macromolecules, an exopolysaccharide biosynthesized by lactic acid bacteria. It has been extensively used as a major component in many types of drug-delivery systems (DDS), which can be submitted to the next in-vivo testing stages, and may be proposed for clinical trials or pharmaceutical use approval. An important aspect to consider in order to maintain high DDS’ biocompatibility is the use of dextran obtained by fermentation processes and with a minimum chemical modification degree. By performing chemical modifications, artefacts can appear in the dextran spatial structure that can lead to decreased biocompatibility or even cytotoxicity. The present review aims to systematize DDS depending on the dextran type used and the biologically active compounds transported, in order to obtain desired therapeutic effects. So far, pure dextran and modified dextran such as acetalated, oxidised, carboxymethyl, diethylaminoethyl-dextran and dextran sulphate sodium, were used to develop several DDSs: microspheres, microparticles, nanoparticles, nanodroplets, liposomes, micelles and nanomicelles, hydrogels, films, nanowires, bio-conjugates, medical adhesives and others. The DDS are critically presented by structures, biocompatibility, drugs loaded and therapeutic points of view in order to highlight future therapeutic perspectives.

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Polygonum tinctorium leaves suppress sodium dextran sulfate-induced colitis through interleukin-10-related pathway

Cited by 3 publications

References 26 publications

Polygonum tinctorium leaf extract ameliorates high-fat diet-induced intestinal epithelial damage in mice

Polygonum tinctorium leaf extract ameliorates high-fat diet-induced intestinal epithelial damage in mice

Immunoregulatory Effects of Elemental Diet and Its Ingredient, Tryptophan, via Activation of the Aryl Hydrocarbon Receptor in Mice

Dextran Formulations as Effective Delivery Systems of Therapeutic Agents

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