Polygonatum polysaccharide attenuates inflammation through inhibiting NLRP3 inflammasome in diabetic cardiomyopathy rats

Abstract

Polygonatum polysaccharide (PP) has good myocardial protection. This study aims to find whether PP can relieve inflammation and play a protective role in diabetic cardiomyopathy (DCM). Intraperitoneal injection of streptozotocin was used to induce DCM in rats, which were then separated into four groups: model group, PP-200 group (200 mg/kg PP), PP-400 group (400 mg/kg PP), and Met group (120 mg/kg metformin). Both control (NC) and model groups of rats were administered normal saline. According to the kit instructions mentioned on the kit, the levels of blood glucose, glycosylated hemoglobin, insulin (INS), and myocardial enzymes (creatinine kinase, B-type natriuretic peptide, and cardiac troponin I) were measured after 8 weeks. Cardiac function was detected by echocardiography. Hematoxylin and eosin (HE) and Masson staining were used to observe pathological changes. Myocardial RNA and protein levels of NLR family pyrin domain containing 3 (NLRP3), caspase-1, and Gasdermin D (GSDMD) were quantified through reverse transcription-polymerase chain reaction and Western blotting analysis. The in vivo findings showed that PP could reduce blood glucose, glycosylated hemoglobin, and INS levels, enhance heart functioning, restore histological alterations and myocardial enzymes, and relieve myocardial fibrosis. Furthermore, PP suppressed the expressions of NLRP3, caspase-1, and GSDMD. PP could reduce inflammation in DCM rats by suppressing NLRP3 inflammasome.

Sesame Seeds: A Nutrient-Rich Superfood

“…Phenolics exhibit a notable antioxidative capacity owing to their ability to generate stable radical intermediates through electron utilization. Owing to their capacity to act as antioxidants, they assume a significant function in stabilizing edible oils and safeguarding against the development of undesirable flavors [38][39][40][41][42]. Recent research indicates that phenolic compounds may significantly impact ailments related to oxidative stress.…”

Pharmacological properties of Polygonatum and its active ingredients for the prevention and treatment of cardiovascular diseases

“…Similarly, other researchers have obtained similar conclusions in a rat model of diabetic cardiomyopathy, demonstrating that PSPs can restore the morphology of myocardial tissues and attenuate myocardial fibrosis. These effects may be mediated through the inhibition of the Nod-like receptor protein 3 (NLRP3) inflammasome [ 155 , 156 ]. In myocardial infarction rats, PSPs were found to improve myocardial injury, reduce inflammatory response, and aid in the repair of ischemic myocardium in acute myocardial infarction rats.…”