Polygenic control of quantitative antibody responsiveness: restrictions of the multispecific effect related to the selection antigen

Ibañez, Olga M.; Mouton, D; Oliveira, S. L.; Fihlo, Orlando G. Ribeiro; Piatti, Rosa Maria; Sant’Anna, Osvaldo A.; Massa, Solange; Biozzi, G; Siqueira, Maria

doi:10.1007/bf00372523

Cited by 20 publications

(21 citation statements)

References 11 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Antibody levels were higher in H IV-A mice. Based on that, the alleles segregated during selective breeding have a nonspecific effect and affect the quantitative antibody responsiveness to many unrelated immunogens (21). The results found in this work were similar to those reported when H IV-A and L IV-A mice were used as experimental models of intracellular parasites such as Leishmania tropica (5), Salmonella typhimurium (6,38), Brucella suis (8) and Paracoccidioides brasiliensis (41).…”

Section: Discussionsupporting

confidence: 79%

“…Female mice from High (H IV-A ) and Low (L IV-A ) lines of Selection IV-A, aged 6-8 weeks, were bred in the animal facilities of the Department of Microbiology and Immunology, Biosciences Institute, UNESP-Botucatu (6,21).…”

Section: Methodsmentioning

confidence: 99%

“…These two lines gave identical antibody responses to the somatic antigen of Salmonella used in Selection IV. Several studies have demonstrated that the selective breeding modifies macrophage function in an opposite direction to that of antibody production for Selections I, II and IV-A (1,5,7,17,21). Macrophages of L mice presented high catabolism of the immunogens with consequent deficiency in their antigen presentation function leading to a low antibody production.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Immune Response to the Rhodococcus equi Infection in High and Low Antibody‐Producing Mice (Selection IV‐A)

Pedrini

Acorci

Pinto

et al. 2005

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

Rhodococcus equi is a Gram‐positive, facultative intracellular bacterium which infects macrophages and causes rhodococcal pneumonia and enteritis in foals. Recently, this agent has been recognized as an opportunistic pathogen for immunocompromised humans. Several murine experimental models have been used to study R. equi infection. High (HIV‐A) and Low (LIV‐A) antibody (Ab)‐producers mice were obtained by bi‐directional genetic selections for their ability to produce antibodies against sheep and human erythrocytes (Selection IV‐A). These lines maintain their phenotypes of high and low responders also for other antigens than those of selection (multispecific effect). A higher macrophage activity in LIV‐A mice has been described for several intracellular infectious agents, which could be responsible for their intense macrophage antigens (Ag)‐handling and low Ab production. Due to these differences, LIV‐A mice were found to exhibit a better performance to trigger an effective immune response towards intracellular pathogens. The objective of this work was to characterize the immune response of Selection IV‐A against R. equi. HIV‐A and LIV‐A mice were infected with 2.0 ×106 CFU of ATCC 33701+R. equi by intravenous route. With regards to bacterial clearance and survival assays, LIV‐A mice were more resistant than HIV‐A mice to virulent R. equi. LIV‐A mice presented a higher hydrogen peroxide (H2O2) and nitric oxide (NO) endogenous production by splenic macrophages than HIV‐A mice. LIV‐A expressed the most intense cellular response, available by the Delayed‐Type Hypersensitivity (DTH) reaction, which activated macrophages and produced more H2O2 and NO. The three times higher specific antibodies titres in HIV‐A indicated that Selection IV‐A maintained the multispecific effect and the polygenic control of humoral and cellular responses also to R. equi.

show abstract

Section: Discussionsupporting

confidence: 79%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Immune Response to the Rhodococcus equi Infection in High and Low Antibody‐Producing Mice (Selection IV‐A)

Pedrini

Acorci

Pinto

et al. 2005

Microbiology and Immunology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Ten eight-week-old female mice from the High IV-A strain (Ibanez et al 1988) Production of an antiserum against bovine Duffy antigen. The secondary structure of the Bos taurus taurus Duffy protein (GenBank AAX09096.1) was generated at SOSUI (http://bp.nuap.…”

Section: Animalsmentioning

confidence: 99%

Detection and quantification of Duffy antigen on bovine red blood cell membranes using a polyclonal antibody

et al. 2012

View full text Add to dashboard Cite

Babesiosis is one of the most important diseases affecting livestock agriculture worldwide. Animals from the subspecies Bos taurus indicus are more resistant to babesiosis than those from Bos taurus taurus. The genera Babesia and Plasmodium are Apicomplexa hemoparasites and share features such as invasion of red blood cells (RBC). The glycoprotein Duffy is the only human erythrocyte receptor for Pasmodium vivax and a mutation which abolishes expression of this glycoprotein on erythrocyte surfaces is responsible for making the majority of people originating from the indigenous populations of West Africa resistant to P. vivax. The current work detected and quantified the Duffy antigen on Bos taurus indicus and Bos taurus taurus erythrocyte surfaces using a polyclonal antibody in order to investigate if differences in susceptibility to Babesia are due to different levels of Duffy antigen expression on the RBCs of these animals, as is known to be the case in human beings for interactions of Plasmodium vivax-Duffy antigen. ELISA tests showed that the antibody that was raised against Duffy antigens detected the presence of Duffy antigen in both subspecies and that the amount of this antigen on those erythrocyte membranes was similar. These results indicate that the greater resistance of B. taurus indicus to babesiosis cannot be explained by the absence or lower expression of Duffy antigen on RBC surfaces.

show abstract

“…Genetically heterogeneous lines of high-and lowresponder mice, which better represent a natural population, are useful for the determination of genetic and immunological parameters of general and specific immune responsiveness. Homozygosity for the high or low production of antibodies was attained by bi-directional selective breeding after several generations and in addition to differences in their responses to the selection antigen, the animals also show high or low responsiveness to non related immunogens (Siqueira et al, 1976;Nilsson et al, 1979;Ibanez et al, 1988;de Franco et al, 1996). As previously stated and recently expressed in an editorial (Boyartchuk & Dietrich, 2002), the advantage of this experimental model in relation to inbred lines of mice is the availability, in the H and L lines, of genetically homogeneous relevant loci controlling the general character, that allow the study of correlations between distinct immunobiological parameters.…”

Section: Introductionmentioning

confidence: 99%

Antibody responsiveness during immunization and challenge of genetically modified antibody responder mice with murine hepatitis virus 3

Vassão

Consales²,

Sant’Anna

et al. 2003

Immunobiology

Self Cite

View full text Add to dashboard Cite

The aim of this study was to evaluate some immunological patterns involved in natural and acquired resistance against MHV3 using the original model of genetically modified lines of mice selected for high (HIII) and low (LIII) antibody responsiveness. As previously shown, a lower pre-existing anti-MHV antibody level was found in susceptible HIII mice as compared to resistant LIII mice. Mortality rates of the F1 (H x L) hybrids and F2 and backcross segregants reflected co-dominance of both characters and the survivors had higher preexisting anti-MHV antibody titers. The present data show that both lines had the potential to synthesize antibodies and that the resistance acquired by the susceptible HIII mice paralleled the antibody synthesis. Nevertheless, higher antibody titers were necessary to confer resistance in HIII mice than in LIII ones. When compared to uvMHV3, a single immunization with a related infectious MHV strain induced a higher antibody synthesis and led the HIII mice to resist the MHV3 challenge. A direct correlation between the antibody level and resistance to infection was always observed in HIII mice. Although mounting a Th2 response as indicated by IgG1 responses, they were also able to readily synthesize large amounts of IgG2a antibodies after immunization or during infection, reflecting a Th1 response. The transfer of anti-MHV antibodies to susceptible HIII mice was capable of conferring resistance to MHV3, providing the antibodies were present before virus infection and in large amounts. The resistance and the survival time of these animals increased with the level of antibody administered. If these direct and clear data suggest that HIII mice can acquire resistance through antibodies, the basis of the resistance of the resistant LIII mice may rely on mechanisms less dependent on antibodies.

show abstract

Polygenic control of quantitative antibody responsiveness: restrictions of the multispecific effect related to the selection antigen

Cited by 20 publications

References 11 publications

Immune Response to the Rhodococcus equi Infection in High and Low Antibody‐Producing Mice (Selection IV‐A)

Immune Response to the Rhodococcus equi Infection in High and Low Antibody‐Producing Mice (Selection IV‐A)

Detection and quantification of Duffy antigen on bovine red blood cell membranes using a polyclonal antibody

Antibody responsiveness during immunization and challenge of genetically modified antibody responder mice with murine hepatitis virus 3

Contact Info

Product

Resources

About