Polygalacturonase and Tomato Fruit Ripening

Giovannoni, James J.; DellaPenna, Dean; Bennett, A. B.; Fischer, Robert L.

doi:10.1002/9780470650509.ch2

Cited by 28 publications

(7 citation statements)

References 89 publications

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“…The main role of PG during fruit ripening is pectin hydrolysis, which leads to softening of the fruit (Rao and Paran , Koyama et al , Ahmed et al ). This line of thinking is supported by the finding in tomato plants that high levels of endo‐PG activity in ripe fruits led to fruit softening (Giovannoni et al ). In this study, PG activities and gene expression were significantly increased during the BR and RM stages in transgenic tomato lines, which suggested that MaMADS7 over‐expression greatly influenced fruit texture (Figs.…”

Section: Discussionmentioning

confidence: 78%

Role for the banana AGAMOUS‐like gene MaMADS7 in regulation of fruit ripening and quality

Liu

Lin

et al. 2015

Physiologia Plantarum

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MADS-box transcription factors play important roles in organ development. In plants, most studies on MADS-box genes have mainly focused on flower development and only a few concerned fruit development and ripening. A new MADS-box gene named MaMADS7 was isolated from banana fruit by rapid amplification of cDNA ends (RACE) based on a MADS-box fragment obtained from a banana suppression subtractive hybridization (SSH) cDNA library. MaMADS7 is an AGAMOUS-like MADS-box gene that is preferentially expressed in the ovaries and fruits and in tobacco its protein product localizes to the nucleus. This study found that MaMADS7 expression can be induced by exogenous ethylene. Ectopic expression of MaMADS7 in tomato resulted in broad ripening phenotypes. The expression levels of seven ripening and quality-related genes, ACO1, ACS2, E4, E8, PG, CNR and PSY1 in MaMADS7 transgenic tomato fruits were greatly increased while the expression of the AG-like MADS-box gene TAGL1 was suppressed. Compared with the control, the contents of β-carotene, lycopene, ascorbic acid and organic acid in transformed tomato fruits were increased, while the contents of glucose and fructose were slightly decreased. MaMADS7 interacted with banana 1-aminocyclopropane-1-carboxylic acid (ACC) oxidase gene 1 (MaACO1) and tomato phytoene synthase gene (LePSY1) promoters. Our results indicated that MaMADS7 plays an important role in initiating endogenous ethylene biosynthesis and fruit ripening.

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Section: Discussionmentioning

confidence: 78%

Role for the banana AGAMOUS‐like gene MaMADS7 in regulation of fruit ripening and quality

Liu

Lin

et al. 2015

Physiologia Plantarum

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“…In addition to pectin, starch degradation is also considered a major pathway for fruit softening (Brady, 1987; Giovannoni et al ., 1992). Expression patterns of genes involved in starch degrading in guava (Figure 6), overwhelmingly showed that starch content decreased with fruit ripening (Figure 4; Table S19).…”

Section: Discussionmentioning

confidence: 99%

A chromosome‐level genome assembly provides insights into ascorbic acid accumulation and fruit softening in guava (Psidium guajava)

Chen

Feng

Lin

et al. 2020

Plant Biotechnology Journal

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Summary Guava (Psidium guajava) is an important fleshy‐fruited tree of the Myrtaceae family that is widely cultivated in tropical and subtropical areas of the world and has attracted considerable attention for the richness of ascorbic acid in its fruits. However, studies on the evolution and genetic breeding potential of guava are hindered by the lack of a reference genome. Here, we present a chromosome‐level genomic assembly of guava using PacBio sequencing and Hi‐C technology. We found that the genome assembly size was 443.8 Mb with a contig N50 of ~15.8 Mb. We annotated a total of 25 601 genes and 193.2 Mb of repetitive sequences for this genome. Comparative genomic analysis revealed that guava has undergone a recent whole‐genome duplication (WGD) event shared by all species in Myrtaceae. In addition, through metabolic analysis, we determined that the L‐galactose pathway plays a major role in ascorbic acid biosynthesis in guava fruits. Moreover, the softening of fruits of guava may result from both starch and cell wall degradation according to analyses of gene expression profiles and positively selected genes. Our data provide a foundational resource to support molecular breeding of guava and represent new insights into the evolution of soft, fleshy fruits in Myrtaceae.

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“…According to the more recent studies, 1‐MCP was effective in delaying fruit softening in various horticultural products such as tomatoes,10, 14, 15, 27 apple,28–31 plum32, 33 and apricot34, 35. Softening is thought to be associated with the degradation of pectic substance in the cell wall caused by accumulation of cell wall hydrolase activities such as pectinesterase (PE), PG and β‐galactosidase (β‐Gal) 36–38. PG is absent in green fruit but increases from the point of color change 39.…”

Section: Discussionmentioning

confidence: 99%

Influence of different concentrations of 1‐methylcyclopropene on the quality of tomato harvested at different maturity stages

Sabır

Agar

2011

J Sci Food Agric

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Polygalacturonase and Tomato Fruit Ripening

Cited by 28 publications

References 89 publications

Role for the banana AGAMOUS‐like gene MaMADS7 in regulation of fruit ripening and quality

Role for the banana AGAMOUS‐like gene MaMADS7 in regulation of fruit ripening and quality

A chromosome‐level genome assembly provides insights into ascorbic acid accumulation and fruit softening in guava (Psidium guajava)

Influence of different concentrations of 1‐methylcyclopropene on the quality of tomato harvested at different maturity stages

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