Polyethylenimine-based non-viral gene delivery systems

Lungwitz, Uta; Breunig, Miriam; Blunk, Torsten; Göpferich, Achim

doi:10.1016/j.ejpb.2004.11.011

Cited by 878 publications

(635 citation statements)

References 200 publications

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“…In addition, PEI has recently released a high degree of purity for Aquasolo, which is formed from 2-ethyl-2-oxazoline (>99.5% by GC) under compression conditions [17]. On the other hand, PEIs are polymer molecules that consist of repeating amine units and two aliphatic carbon.…”

Section: Peimentioning

confidence: 99%

Polyethylenimine-based nanocarriers in co-delivery of drug and gene: a developing horizon

Zakeri

Kouhbanani

Beheshtkhoo

et al. 2018

Nano Reviews & Experiments

203

137

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The meaning of gene therapy is the delivery of DNA or RNA to cells for the treatment or prevention of genetic disorders. The success rate of gene therapy depends on the progression and safe gene delivery system. The vectors available for gene therapy are divided into viral and non-viral systems. Viral vectors cause higher transmission efficiency and long gene expression, but they have major problems, such as immunogenicity, carcinogenicity, the inability to transfer large size genes and high costs. Non-viral gene transfer vectors have attracted more attention because they exhibit less toxicity and the ability to transfer large size genes. However, the clinical application of non-viral methods still faces some limitations, including low transmission efficiency and poor gene expression. In recent years, numerous methods and gene-carriers have been developed to improve gene transfer efficiency. The use of Polyethylenimine (PEI) based transfer of collaboration may create a new way of treating diseases and the combination of chemotherapy and gene therapy. The purpose of this paper is to introduce the PEI as an appropriate vector for the effective gene delivery.

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Section: Peimentioning

confidence: 99%

Polyethylenimine-based nanocarriers in co-delivery of drug and gene: a developing horizon

Zakeri

Kouhbanani

Beheshtkhoo

et al. 2018

Nano Reviews & Experiments

203

137

View full text Add to dashboard Cite

show abstract

“…2) and in the literature to be increased by complexation with PEI [5][6][7]. This study seeks to test the hypothesis that when used together, ultrasound and PEI can have a synergistic effect to increase DNA transfection.…”

Section: Effect Of Ultrasound On Transfection Of Aosmc By Pei:dna Commentioning

confidence: 99%

“…One of the most promising non-viral vectors is poly(ethylenimine) (PEI), which is the most widely used gene delivery vector among cationic polymers [5][6][7]. PEI has been shown to be a relatively efficient gene transfer agent without the need for additional endosomolytic or lysosomotropic agents [8].…”

Section: Introductionmentioning

confidence: 99%

Synergistic effect of ultrasound and PEI on DNA transfection in vitro

Deshpande

Prausnitz

2007

Journal of Controlled Release

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Ultrasound and poly(ethylenimine) (PEI) have each separately been shown to increase DNA transfection efficiency. This study tested the hypothesis that the combination of ultrasound and PEI can have a synergistic effect to increase DNA transfection. This in vitro study assessed transfection efficiency of two different DNA plasmids encoding green fluorescent protein and firefly luciferase in two different cells types, a primary culture of human aortic smooth muscle cells and an immortal line of human prostrate cancer cells. We found that ultrasound sonication increased transfection up to 18-fold, DNA complexation with PEI increased transfection up to 90-fold, and the combination of ultrasound and PEI synergistically increased transfection up to 200-fold, which resulted in reporter gene expression by 34% of cells. Kinetic measurements found that the effects of ultrasound alone acted quickly, whereas increased transfection by PEI either alone or in combination with ultrasound strongly benefited from a 4-h incubation with the DNA plasmid after sonication. Although serum reduced absolute expression levels, it did not affect the relative increase in transfection when ultrasound was added to PEI enhancement. Flow cytometry measurements showed that sonication increased intracellular uptake of labelled DNA complexed to PEI by 55% relative to PEI complexation alone. Electrophoresis assay showed no damage to DNA or PEI-DNA complexes after sonication. Overall, these results suggest that the combination of ultrasound and PEI can have a synergistic effect to increase DNA transfection.

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“…Branched polyethylenimine (br-PEI), which is still the "gold-standard" cationic polymer, was used for comparison. 1 The tube was sealed with a rubber septum and subsequently purged with dry argon for 20 min. Then, a degassed mixture of OEGMA (120 -n equiv), DMAEMA (n equiv), and ethanol (monomers/solvent ∼ 1:1.25 v/v) were added through the septum with a degassed syringe.…”

Section: Introductionmentioning

confidence: 99%

Characterization of Tailor-Made Copolymers of Oligo(ethylene glycol) Methyl Ether Methacrylate and N,N-Dimethylaminoethyl Methacrylate as Nonviral Gene Transfer Agents: Influence of Macromolecular Structure on Gene Vector Particle Properties and Transfection Efficiency

et al. 2009

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Oligo(ethylene glycol) methyl ether methacrylates (OEGMA) of various chain lengths (i.e., 9, 23, or 45 EG units) and N,N-dimethylaminoethyl methacrylate (DMAEMA) were copolymerized by atom transfer radical polymerization (ATRP), yielding well-defined P(DMAEMA-co-OEGMA) copolymers with increasing OEGMA molar fractions (F OEGMA) but a comparable degree of polymerization (DP ∼ 120). Increase of both F OEGMA and OEGMA chain lengths correlated inversely with gene vector size, morphology, and zeta potential. P(DMAEMA-co-OEGMA) copolymers prevented gene vector aggregation at high plasmid DNA (pDNA) concentrations in isotonic solution and did not induce cytotoxicity even at high concentrations. Transfection efficiency of the most efficient P(DMAEMA-co-OEGMA) copolymers was found to be >10-fold lower compared with branched polyethylenimine (PEI) 25 kDa. Although OEGMA copolymerization largely reduced gene vector binding with the cell surface, cellular internalization of the bound complexes was less affected. These observations suggest that inefficient endolysosomal escape limits transfection efficiency of P(DMAEMA-co-OEGMA) copolymer gene vectors. Despite this observation, optimized p(DMAEMA-co-OEGMA) gene vectors remained stable under conditions for in vivo application leading to 7-fold greater gene expression in the lungs compared with PEI. Tailor-made P(DMAEMA-co-OEGMA) copolymers are promising nonviral gene transfer agents that fulfill the requirements for successful in vivo gene delivery.

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Polyethylenimine-based non-viral gene delivery systems

Cited by 878 publications

References 200 publications

Polyethylenimine-based nanocarriers in co-delivery of drug and gene: a developing horizon

Polyethylenimine-based nanocarriers in co-delivery of drug and gene: a developing horizon

Synergistic effect of ultrasound and PEI on DNA transfection in vitro

Characterization of Tailor-Made Copolymers of Oligo(ethylene glycol) Methyl Ether Methacrylate and N,N-Dimethylaminoethyl Methacrylate as Nonviral Gene Transfer Agents: Influence of Macromolecular Structure on Gene Vector Particle Properties and Transfection Efficiency

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