Polyethylenimine: A new differentiation factor to endothelial/cardiac tissue

Esmaeili

et al. 2021

J Mater Sci: Mater Med

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Curcumin has been recognized as an effective anticancer agent. However, due to its hydrophobic property, the cell absorption is not satisfied. Herein, the curcumin nanoparticles were prepared in the presence of polyethylene glycol 6000 (PEG6000) to reduce its elimination by immune system. For first time, not only the curcumin was encapsulated within the niosome nanoparticles modified by PEG, there are no reports related to the anticancer property of curcumin against thyroid cancers. The nanoparticles was developed and its anticancer was studied on sw-1736 cancer cell line. The nanoparticles were examined by scanning electron microscopy (SEM) and dynamic light scattering (DLS). Also, the release profile of curcumin, the IC50 concentration, the radical amount and the gene expression were evaluated. The optimized nanoparticles showed a diameter of 212 ± 31 nm by SEM and the encapsulation efficiency and loading capacity of 76% and 16.8% respectively. DLS confirmed the polydispersity index (PDI) of 0.596 and the release model was shown a sustained release with the delivery of 68% curcumin after 6 days. Also, the nanoparticles indicated the higher storage stability at 4 °C. After the cell treatment, the apoptotic bodies were appeared and IC50 was obtained as 0.159 mM. Moreover, the generated radicals by the treated cells was 86% after 72 h and the gene pattern indicated the bax/bcl2 ratio of 6.83 confirming the apoptosis effect of the nanoparticles. The results approved the nanoparticles could be suggested as an anticancer drug candidate for thyroid cancers.

Section: Methodsmentioning

confidence: 99%

Polyethylene glycol triggers the anti-cancer impact of curcumin nanoparticles in sw-1736 thyroid cancer cells

Hosseinzadeh

Esmaeili

et al. 2021

J Mater Sci: Mater Med

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“…Nowadays, a vast area of researches has been devoted to develop novel scaffolds for cardiac tissue engineering using nanoscale fabrication techniques specially the electrospinning methods. This methods helps to fabricate different kinds of nanofibrous scaffolds using natural or synthetic materials . Although these scaffolds provide a microenvironment for cells like myocardium extra cellular matrix, achieving a functional myocardium is still remained as a big challenge.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of graphene‐silver/polyurethane nanofibrous scaffolds for cardiac tissue engineering

Polymers for Advanced Techs

Azadi

Hatamie

et al. 2019

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The graphene-based nanocomposites are considered as great candidates for enhancing electrical and mechanical properties of nonconductive scaffolds in cardiac tissue engineering. In this study, reduced graphene oxide-silver (rGO-Ag) nanocomposites(1 and 2 wt%) were synthesized and incorporated into polyurethane (PU) nanofibers via electrospinning technique. Next, the human cardiac progenitor cells (hCPCs) were seed on these scaffolds for in vitro studies. The rGO-Ag nanocomposites were studied by X-ray diffraction (XRD), Raman spectroscopy, and transmission electron microscope (TEM). After incorporation of rGO-Ag into PU nanofibers, the related characterizations were carried out including scanning electron microscope (SEM), TEM, water contact angle, and mechanical properties. Furthermore, PU and PU/nanocomposites scaffolds were used for in vitro studies, wherein hCPCs showed good cytocompatibility via 3-(4, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and considerable attachment on the scaffold using SEM studies. Real-time polymerase chain reaction (PCR) and immunostaining studies confirmed the upregulation of cardiac specific genes including GATA-4, T-box 18 (TBX 18), cardiac troponin T (cTnT), and alphamyosin heavy chain (α-MHC) in the PU/rGO-Ag scaffolds in comparison with neat PU ones. Therefore, these nanofibrous rGO-Ag-reinforced PU scaffolds can be considered as suitable candidates in cardiac tissue engineering.

“…Nowadays, electrospinning is considered as a well‐known nanoscale fabrication technique for design and synthesis of fibrous scaffolds for cardiac tissue engineering. In order to mimic the extracellular matrix of the myocardium to support cardiac cells, natural or synthetic nanofibers can be produced using the electrospinning method . Although different types of biocompatible natural/synthetic scaffolds have been fabricated during the last decade, still they cannot induce an acceptable level of maturity and cardiogenic differentiation.…”

Section: Introductionmentioning

confidence: 99%

Incorporation of two‐dimensional nanomaterials into silk fibroin nanofibers for cardiac tissue engineering

Polymers for Advanced Techs

Heirani‐Tabasi

Hajiabbas

et al. 2019

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Mimicking the extracellular matrix to have a similar nanofibrous structure regarding electrical conductivity and mechanical properties would be highly beneficial for cardiac tissue engineering. The molybdenum disulfide, MoS 2 , and reduced graphene oxide, rGO, nanosheets are two-dimensional nanomaterials which can be considered as great candidates for enhancing the electrical and mechanical properties of biological scaffolds for cardiac tissue engineering applications. In this study, MoS 2 and rGO nanosheets were synthesized and incorporated into silk fibroin nanofibers, SF, via electrospinning method. Then, the human iPSCs transfected with TBX-18 gene, TBX18-hiPSCs, were seeded on these scaffolds for in vitro studies. The MoS 2 and rGO nanosheets were studied by Raman spectroscopy. After incorporation of the nanosheets into SF nanofibers, the associated characterizations were carried out including scanning electron microscopy, transmission electron microscopy, water contact angle, and mechanical test. Furthermore, SF, SF/MoS 2 , and SF/rGO scaffolds were used for in vitro studies. Herein, the scaffolds exhibited acceptable biocompatibility and considerable attachment to TBX18-hiPSCs confirmed by 3-(4, 5dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide, MTT, assay, and scanning electron microscopy. Also, the real-time PCR and immunostaining studies confirmed the maturity and upregulation of cardiac functional genes, including GATA-4, c-TnT, and α-MHC in the SF/MoS 2 and SF/rGO scaffolds compared with the bare SF one.Therefore, the reinforcement of these SF-based scaffolds with MoS 2 and rGO endues them as a suitable candidate for cardiac tissue engineering.