Polyethylene Glycol Functionalized Nanoparticles for Improved Cancer Treatment

Cruje, Charmainne; Chithrani, Devika B.

doi:10.1166/rnn.2014.1042

Cited by 52 publications

(68 citation statements)

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“…Opsonin is a protein that binds to foreign microorganisms for enhanced phagocytosis and removal of foreign material from circulation for clearance. Protecting NP surfaces from binding of opsonin can improve blood circulation of the NPs [ 5 , 6 ]. Prolonged circulation of PEG coated particles promote tumor selectivity of NPs [ 7 ].…”

Section: Introductionmentioning

confidence: 99%

Peptide Mediated In Vivo Tumor Targeting of Nanoparticles through Optimization in Single and Multilayer In Vitro Cell Models

Yang

Bromma

Chithrani

2018

Cancers

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Optimizing the interface between nanoparticles (NPs) and the biological environment at various levels should be considered for improving delivery of NPs to the target tumor area. For NPs to be successfully delivered to cancer cells, NPs needs to be functionalized for circulation through the blood vessels. In this study, accumulation of Polyethylene Glycol (PEG) functionalized gold nanoparticles (GNPs) was first tested using in vitro monolayer cells and multilayer cell models prior to in vivo models. A diameter of 10 nm sized GNP was selected for this study for sufficient penetration through tumor tissue. The surfaces of the GNPs were modified with PEG molecules, to improve circulation time by reducing non-specific uptake by the reticuloendothelial system (RES) in animal models, and with a peptide containing integrin binding domain, RGD (arginyl-glycyl-aspartic acid), to improve internalization at the cellular level. A 10–12% accumulation of the injected GNP dose within the tumor was observed in vivo and the GNPs remained within the tumor tissue up to 72 h. This study suggests an in vitro platform for optimizing the accumulation of NP complexes in cells and tissue structures before testing them in animal models. Higher accumulation within the tumor in vivo upon surface modification is a promising outcome for future applications where GNPs can be used for drug delivery and radiation therapy.

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Section: Introductionmentioning

confidence: 99%

Peptide Mediated In Vivo Tumor Targeting of Nanoparticles through Optimization in Single and Multilayer In Vitro Cell Models

Yang

Bromma

Chithrani

2018

Cancers

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“…Polymers, such as polyamides, overcome these drawbacks through blending or incorporation into copolymers, or by modification of their side-chains to introduce the desired physical properties [10][11][12][13]. Polyethylene glycol (PEG) has also proven to be a synthetic polymer with excellent biocompatibility for use in many biomedical applications [14,15]. In drug delivery, conjugation of PEG to therapeutic agents (i.e.…”

Section: Introductionmentioning

confidence: 99%

Potentially biocompatible polyacrylamides derived by the Ugi four-component reaction

Sehlinger

Ochsenreither

Bartnick

et al. 2015

European Polymer Journal

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“…However, the conformational states of PEG on the nanoparticle surface can be complex due to multilayer depositions and interactions between these layers, as shown by Figure 1 A. Therefore there could be a concentration of PEG close to the mushroom to brush transition density, that would form the optimal level of interactions between the polymers, utilizing the steric repulsion of the brush conformation and the topographical inhibition of protein binding provided by the mushroom conformation [ 19 ]. In the systems prepared in this study, this optimal concentration (6% PEG) provides the desired solubility and stealth effects due to the PEG shield, while the cationic properties of chitosan are still retained by the entire system, as shown by Table 1 and Figure 1 F.…”

Section: Resultsmentioning

confidence: 99%

“…However, there is no current consensus on the optimal surface PEGylation density of nanoparticles that would induce and sustain favourable surface properties. Studies have shown that the structure of PEG occurs in two main conformations i.e., mushroom and brush [ 19 ] ( Figure 1 ) with freedom of conformational mobility of PEG chains from attachment point on the core to the surface of nanoparticles in the brush conformation, while there is more lateral interaction in the mushroom configuration [ 6 ].…”

Section: Introductionmentioning

confidence: 99%

Comparative Nanofabrication of PLGA-Chitosan-PEG Systems Employing Microfluidics and Emulsification Solvent Evaporation Techniques

et al. 2020

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Poor circulation stability and inadequate cell membrane penetration are significant impediments in the implementation of nanocarriers as delivery systems for therapeutic agents with low bioavailability. This research discusses the fabrication of a biocompatible poly(lactide-co-glycolide) (PLGA) based nanocarrier with cationic and hydrophilic surface properties provided by natural polymer chitosan and coating polymer polyethylene glycol (PEG) for the entrapment of the hydrophobic drug disulfiram. The traditional emulsification solvent evaporation method was compared to a microfluidics-based method of fabrication, with the optimisation of the parameters for each method, and the PEGylation densities on the experimental nanoparticle formulations were varied. The size and surface properties of the intermediates and products were characterised and compared by dynamic light scattering, scanning electron microscopy and X-ray diffraction, while the thermal properties were investigated using thermogravimetric analysis and differential scanning calorimetry. Results showed optimal particle properties with an intermediate PEG density and a positive surface charge for greater biocompatibility, with nanoparticle surface characteristics shielding physical interaction of the entrapped drug with the exterior. The formulations prepared using the microfluidic method displayed superior surface charge, entrapment and drug release properties. The final system shows potential as a component of a biocompatible nanocarrier for poorly soluble drugs.

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Polyethylene Glycol Functionalized Nanoparticles for Improved Cancer Treatment

Cited by 52 publications

References 0 publications

Peptide Mediated In Vivo Tumor Targeting of Nanoparticles through Optimization in Single and Multilayer In Vitro Cell Models

Peptide Mediated In Vivo Tumor Targeting of Nanoparticles through Optimization in Single and Multilayer In Vitro Cell Models

Potentially biocompatible polyacrylamides derived by the Ugi four-component reaction

Comparative Nanofabrication of PLGA-Chitosan-PEG Systems Employing Microfluidics and Emulsification Solvent Evaporation Techniques

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