Polyethylene glycol-based bidentate ligands to enhance quantum dot and gold nanoparticle stability in biological media

Mei, Bingchu; Susumu, Kimihiro; Medintz, Igor L.; Mattoussi, Hedi

doi:10.1038/nprot.2008.243

Cited by 192 publications

(203 citation statements)

References 41 publications

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“…Ligands can be designed with multiple surface-binding groups (e.g., thiols, which form dative covalent bonds with many metals) to ensure strong and lasting binding: for example, bidentate dihydrolipoic acid (DHLA)-based (44) and tridentate tris(mercaptomethyl) ligands (45). Short ethylene glycol segments can also be included to control nonspecific adsorption (46). Zwitterionic ligands yield exceptionally stable nanoparticles and are the state of the art for compactness and fouling resistance, particularly when combined with novel phase transfer routes like photoligation (47).…”

Section: Nanoparticle Surfacesmentioning

confidence: 99%

Colloidal nanoparticles as advanced biological sensors

Howes

Chandrawati

Stevens

2014

Science

878

686

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Colloidal nanoparticle biosensors have received intense scientific attention and offer promising applications in both research and medicine. We review the state of the art in nanoparticle development, surface chemistry, and biosensing mechanisms, discussing how a range of technologies are contributing toward commercial and clinical translation. Recent examples of success include the ultrasensitive detection of cancer biomarkers in human serum and in vivo sensing of methyl mercury. We identify five key materials challenges, including the development of robust mass-scale nanoparticle synthesis methods, and five broader challenges, including the use of simulations and bioinformatics-driven experimental approaches for predictive modeling of biosensor performance. The resultant generation of nanoparticle biosensors will form the basis of high-performance analytical assays, effective multiplexed intracellular sensors, and sophisticated in vivo probes.Evolution has given rise to organisms of staggering complexity. Our now extensive knowledge of biological systems pales in comparison to the remaining mysteries. Unraveling these requires tools that probe the molecular machinery of life and provide detailed feedback on complex networks of subtle interactions. Such tools are cornerstones of biomedical research and practice, and improvements in these lead directly to a better understanding of fundamental biology, monitoring of health, and diagnosis of disease. Colloidal nanoparticle biosensors are a class of biological probe that will not only yield improved biological sensing but also provide a step change in our ability to probe the biomolecular realm. Nanoparticles can act as high-performance sensors because nanomaterials exhibit unique and useful behaviors not present in their bulk form: for example, bright tunable fluorescence from semiconductor nanoparticles and localized surface plasmon resonance (LSPR) phenomena in metallic nanoparticles. These particles exhibit intense responses to incident light (or other stimuli), and the ability to modulate this response by interaction with target analytes makes them excellent biosensor signal transducers. Outputs can be quantitative or qualitative depending on the functionality of

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Section: Nanoparticle Surfacesmentioning

confidence: 99%

Colloidal nanoparticles as advanced biological sensors

Howes

Chandrawati

Stevens

2014

Science

878

686

View full text Add to dashboard Cite

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“…1 A central strategy used to achieve such control is the passivation of NC surfaces by hydrophobic ligands, such as oleic acid or octadecylphosphonic acid. 2 However, for many applications it is necessary to replace the generic hydrophobic ligands with specific functional ligands, e.g., in order to facilitate aqueous dispersion, 3 enable specific targeting 4 or 20 add complementary optoelectronic functionality. 5 It remains a significant challenge to find general and efficient mechanisms for such ligand exchange reactions.…”

Section: Synthesized Tms Mixed Chalcogenides Leverage Preferential Rementioning

confidence: 99%

Driving oxygen coordinated ligand exchange at nanocrystal surfaces using trialkylsilylated chalcogenides

et al. 2011

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A general, efficient method is demonstrated for exchanging native oxyanionic ligands on inorganic nanocrystals with functional trimethylsilylated (TMS) chalcogenido ligands. In addition, newly synthesized TMS mixed chalcogenides leverage preferential reactivity of TMS-S bonds over TMS-O bonds, enabling efficient transfer of luminescent nanocrystals into aqueous media with retention of their optical properties.

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“…Beyond direct His n -QD self-assembly, concomitant synthesis has developed a family of polyethylene glycol (PEG)-based QD surface functionalizing ligands or caps that are pH stable and that provide access to carbodiimide (EDC)-based linkages or biotinϪavidin chemistry. 25 Other research groups have contributed numerous and quite diverse QD bioconjugation strategies ranging from reactive PEG chemistries to adapting Tsien's CrAsH arsenicϪtetracysteine linkage chemistry along with demonstrating monovalent labeling.…”

mentioning

confidence: 99%

Quantum Dot Peptide Biosensors for Monitoring Caspase 3 Proteolysis and Calcium Ions

Prasuhn¹,

et al. 2010

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The nanoscale size and unique optical properties of semiconductor quantum dots (QDs) have made them attractive as central photoluminescent scaffolds for a variety of biosensing platforms. In this report we functionalize QDs with dye-labeled peptides using two different linkage chemistries to yield Förster resonance energy transfer (FRET)-based sensors capable of monitoring either enzymatic activity or ionic presence. The first sensor targets the proteolytic activity of caspase 3, a key downstream effector of apoptosis. This QD conjugate utilized carbodiimide chemistry to covalently link dye-labeled peptide substrates to the terminal carboxyl groups on the QD’s surface hydrophilic ligands in a quantitative manner. Caspase 3 cleaved the peptide substrate and disrupted QD donor-dye acceptor FRET providing signal transduction of enzymatic activity and allowing derivation of relevant Michaelis−Menten kinetic descriptors. The second sensor was designed to monitor Ca2+ ions that are ubiquitous in many biological processes. For this sensor, Cu+-catalyzed [3 + 2] azide−alkyne cycloaddition was exploited to attach a recently developed azide-functionalized CalciumRuby-Cl indicator dye to a cognate alkyne group present on the terminus of a modified peptide. The labeled peptide also expressed a polyhistidine sequence, which facilitated its subsequent metal-affinity coordination to the QD surface establishing the final FRET sensing construct. Adding exogenous Ca2+ to the sensor solution increased the dyes fluorescence, altering the donor−acceptor emission ratio and manifested a dissociation constant similar to that of the native dye. These results highlight the potential for combining peptides with QDs using different chemistries to create sensors for monitoring chemical compounds and biological processes.

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Polyethylene glycol-based bidentate ligands to enhance quantum dot and gold nanoparticle stability in biological media

Cited by 192 publications

References 41 publications

Colloidal nanoparticles as advanced biological sensors

Colloidal nanoparticles as advanced biological sensors

Driving oxygen coordinated ligand exchange at nanocrystal surfaces using trialkylsilylated chalcogenides

Quantum Dot Peptide Biosensors for Monitoring Caspase 3 Proteolysis and Calcium Ions

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