Polyester Fleeces used as an Artificial Interstitium Influence the Spatial Growth of Regenerating Tubules

Glashauser, Anne; Denk, Lucia; Minuth, Will W.

doi:10.4172/2157-7552.1000105

Cited by 4 publications

(4 citation statements)

References 60 publications

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“…Recent data further illustrate that developing tubules avoid a direct contact to each other by keeping a discrete distance during spatial development. The separation seems to be caused by an interaction between the basal lamina of generating tubules, newly synthesized fibers of the extracellular matrix and neighboring polyester fibers of the fleece [116–122].…”

Section: Reviewmentioning

confidence: 99%

Supportive development of functional tissues for biomedical research using the MINUSHEET® perfusion system

Minuth

Denk

2012

Clinical & Translational Med

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Functional tissues generated under in vitro conditions are urgently needed in biomedical research. However, the engineering of tissues is rather difficult, since their development is influenced by numerous parameters. In consequence, a versatile culture system was developed to respond the unmet needs. Optimal adhesion for cells in this system is reached by the selection of individual biomaterials. To protect cells during handling and culture, the biomaterial is mounted onto a MINUSHEET® tissue carrier. While adherence of cells takes place in the static environment of a 24 well culture plate, generation of tissues is accomplished in one of several available perfusion culture containers. In the basic version a continuous flow of always fresh culture medium is provided to the developing tissue. In a gradient perfusion culture container epithelia are exposed to different fluids at the luminal and basal sides. Another special container with a transparent lid and base enables microscopic visualization of ongoing tissue development. A further container exhibits a flexible silicone lid to apply force onto the developing tissue thereby mimicking mechanical load that is required for developing connective and muscular tissue. Finally, stem/progenitor cells are kept at the interface of an artificial polyester interstitium within a perfusion culture container offering for example an optimal environment for the spatial development of renal tubules. The system presented here was evaluated by various research groups. As a result a variety of publications including most interesting applications were published. In the present paper these data were reviewed and analyzed. All of the results point out that the cell biological profile of engineered tissues can be strongly improved, when the introduced perfusion culture technique is applied in combination with specific biomaterials supporting primary adhesion of cells.

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Section: Reviewmentioning

confidence: 99%

Supportive development of functional tissues for biomedical research using the MINUSHEET® perfusion system

Minuth

Denk

2012

Clinical & Translational Med

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“…It can be applied to cotton, polyester, [79] wool, silk or rayon. Stain release was designed to mimic the natural characteristics of a plant's leaves.…”

Section: Resistsmentioning

confidence: 99%

Nanotextiles- A Broader Perspective

Jeevani¹

2011

J Nanomed Nanotechnol

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Nano Technology means optimizing performance and providing smart solutions for the future. It means configuring molecules to change in size and properties for enhancement as in the case of smart fabrics. Smart fabrics can help manufacturers and designers with the increased emphasis on lifestyle, aesthetic appeal and form demanded for technological products. Nanosize particles can exhibit unexpected properties different from those of the bulk material. The basic premise is that properties can dramatically change when a substance's size is reduced to the nanometer range. Nanotechnology has versatile applications in Textile Chemicals industry in manufacturing garments with stain resistance, flame retardant finishes, wrinkle resistance finishes, moisture management, antimicrobial qualities, UV protection, and soil release properties. Incorporating nanomaterials into a textile can affect a host of properties, including shrinkage, strength, electrical conductivity and flammability. Nanotechnology has also made a tremendous impact on functionality and performance. Nano-treated textiles may lead to many inventions as the science develops in future.

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“…All up to date performed experiments yet point out that development of tubules is triggered by interactions between their basal lamina, newly synthesized fibers of the extracellular matrix and fibers of the polyester fleece (Minuth et al 2010a, b, c, d;Miess et al 2010;Glashauser et al 2011).…”

Section: Regeneration Of Renal Parenchymamentioning

confidence: 99%

Bridging the gap between traditional cell cultures and bioreactors applied in regenerative medicine: practical experiences with the MINUSHEET perfusion culture system

Minuth

Denk

2015

Cytotechnology

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To meet specific requirements of developing tissues urgently needed in tissue engineering, biomaterial research and drug toxicity testing, a versatile perfusion culture system was developed. First an individual biomaterial is selected and then mounted in a MINUSHEET Ò tissue carrier. After sterilization the assembly is transferred by fine forceps to a 24 well culture plate for seeding cells or mounting tissue on it. To support spatial (3D) development a carrier can be placed in various types of perfusion culture containers. In the basic version a constant flow of culture medium provides contained tissue with always fresh nutrition and respiratory gas. For example, epithelia can be transferred to a gradient container, where they are exposed to different fluids at the luminal and basal side. To observe development of tissue under the microscope, in a different type of container a transparent lid and base are integrated. Finally, stem/progenitor cells are incubated in a container filled by an artificial interstitium to support spatial development. In the past years the described system was applied in numerous own and external investigations. To present an actual overview of resulting experimental data, the present paper was written.

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Polyester Fleeces used as an Artificial Interstitium Influence the Spatial Growth of Regenerating Tubules

Cited by 4 publications

References 60 publications

Supportive development of functional tissues for biomedical research using the MINUSHEET® perfusion system

Supportive development of functional tissues for biomedical research using the MINUSHEET® perfusion system

Nanotextiles- A Broader Perspective

Bridging the gap between traditional cell cultures and bioreactors applied in regenerative medicine: practical experiences with the MINUSHEET perfusion culture system

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