Polyelectrolyte Multicomponent Colloidosomes Loaded with Nisin Z for Enhanced Antimicrobial Activity against Foodborne Resistant Pathogens

Niaz, Taskeen; Shabbir, Saima; Nооr, Tayyaba; Abbasi, Rashda; Raza, Zulfiqar Ali; Imran, Muhammad

doi:10.3389/fmicb.2017.02700

Cited by 52 publications

(27 citation statements)

References 58 publications

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“…One of the most accepted mechanisms of nisin activity, as an antimicrobial compound, is its interaction with the anionic lipids present in the cytoplasmic membrane that promote a disturbance, forming pores that cause alteration in the vital ions gradient, leading to cell death (Breukink & de Kruijff, 2006;Tong, Ni & Ling, 2014). The MIC for the bacteria L. innocua and S. aureus were in agreement with inhibition concentrations reported by other authors (Niaz et al, 2018;Ramos et al, 2012;Sadiq et al, 2016;Yoneyama, Fukao, Zendo, Nakayama & Sonomoto, 2008). In addition, the MIC determined for S. enterica Choleraesuis was above the values found in other studies, however, with slight changes of microbial species and types of nisin (Field et al, 2012;Kim, Jung, Kim & Shin, 2006).…”

Section: Minimum Inhibitory Concentration (Mic) Of Nisin Zsupporting

confidence: 88%

Biodegradable film development by nisin Z addition into hydroxypropylmethylcellulose matrix for mozzarella cheese preservation

Freitas

Silva

Oliveira

et al. 2020

IJFS

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Currently, improvement of food preservation has been a substantial challenge for industries to increase shelf-life of products and to maintain food quality during storage. These goals are often tied to the sustainable tendency for use of eco-friendly packaging to store these products without loss of the packaging features. Therefore, the aim of this study was to produce biodegradable antimicrobial films by the incorporation of nisin Z peptide under different concentrations (0 %, 5 %, 10 %, 15 % and 20 % wt.) into hydroxypropylmethylcellulose (HPMC) matrices. The active film properties were evaluated in terms of their antimicrobial capacity in vitro, mechanical performance and microscopic characteristics. Hence, active films containing 10 % (wt.) of nisin Z and control films were placed in contact with sliced mozzarella cheese for eight days, and microbiological growth was monitored during storage. Nisin Z’s antimicrobial effects were observed against the Gram-positive microorganisms such as Staphylococcus aureus and Listeria innocua, regardless if the compound was free as a suspension or incorporated into HPMC matrices. However, the expected low action of nisin Z against Gram-negative bacteria, as reported in literature, was not observed since Salmonella enterica Choleraesuis’s growth was inhibited. Moreover, active films with added nisin Z (10 % wt.) were more effective than the control film to inhibit mesophilic microorganisms in mozzarella cheese during 8 days of storage. The mechanical properties of the films were not influenced by nisin Z incorporation, since the addition of the compound enhanced the active function without the loss of mechanical properties required for a good food packaging. These results suggest that biodegradable films produced by nisin Z addition into HPMC matrix are an excellent biomaterial for mozzarella cheese preservation.

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Section: Minimum Inhibitory Concentration (Mic) Of Nisin Zsupporting

confidence: 88%

Biodegradable film development by nisin Z addition into hydroxypropylmethylcellulose matrix for mozzarella cheese preservation

Freitas

Silva

Oliveira

et al. 2020

IJFS

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“…In recent times, nanotechnology has provided great assistance to improve the microbiological safety of food and feed matrices. Particularly, nanomaterials of chitosan were found eco-friendly and safe, and their application in food is highly satisfactory by consumer, food industry, and regulatory agencies ( Yeung et al, 2016 ; Niaz et al, 2017 ; Siddaiah et al, 2018 ). Furthermore, essential oils are volatile and lose their stability and bio-functional features on exposure to temperature, light, pH, moisture, and oxygen ( Jamil et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Antifungal Activity of Chitosan Nanoparticles Encapsulated With Cymbopogon martinii Essential Oil on Plant Pathogenic Fungi Fusarium graminearum

Kalagatur

Ghosh

Sundararaj³

et al. 2018

Front. Pharmacol.

148

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Application of synthetic fungicides in agricultural commodities has been restricted due to development of fungicide resistance fungi and deleterious impact on environment and health of farm animals and humans. Hence, there is an urge for development of mycobiocides, and the present study was undertaken to determine the antifungal activity of Cymbopogon martinii essential oil (CMEO) on post-harvest pathogen Fusarium graminearum. The CMEO was extracted by hydrodistillation and GC-MS chemical profile revealed the presence of 46 compounds and abundant was geraniol (19.06%). The minimum inhibitory concentration and minimum fungicidal concentration of CMEO were determined as 421.7 ± 27.14 and 618.3 ± 79.35 ppm, respectively. The scanning electron microscopic observation of CMEO exposed macroconidia was exhibited a detrimental morphology with vesicles, craters, protuberance, and rough surfaces related to control fungi. The CMEO induced the death of fungi through elevating intracellular reactive oxygen species and lipid peroxidation, and depleting ergosterol content. Regrettably, essential oils are highly volatile and become unstable and lose their biological features on exposure to light, heat, pH, moisture, and oxygen. To overcome these issues, chitosan encapsulated CMEO nanoparticles (Ce-CMEO-NPs) were prepared. The synthesized Ce-CMEO-NPs have spherical morphology with Zeta potential of 39.3–37.2 mV and their corresponding size was found in range of 455–480 nm. The Fourier transform infrared analysis confirmed that bio-active constituents of CMEO were well stabilized due to chitosan conjugation and successfully formed Ce-CMEO-NPs. The in vitro release assay observed that the release of CMEO is stabilized due to the complex formation with chitosan and thereby, increases the lifetime antifungal activity of CMEO by gradual release of antifungal constituents of Ce-CMEO-NPs. In conclusion, antifungal and antimycotoxin activities of CMEO and Ce-CMEO-NPs against F. graminearum were assessed in maize grains under laboratory conditions over a storage period of 28 days. Interestingly, Ce-CMEO-NPs were presented efficient and enhanced antifungal and antimycotoxin activities related to CMEO, and it could be due to perseverance of antifungal activity by controlled release of antifungal constituents from Ce-CMEO-NPs. The study concluded that Ce-CMEO-NPs could be highly appropriate as mycobiocides in safeguarding the agricultural commodities during storage period in agricultural and food industries.

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“…Elemental analysis values were recorded on Model ANALYST 2000 CHNS, Perkin Elmer Analyzer. Multiskan™ GO Microplate Spectrophotometer was used to quantify synthesized compounds for accuracy, precision and sensitivity [26].…”

Section: Methodsmentioning

confidence: 99%

Designing, synthesis and characterization of 2-aminothiazole-4-carboxylate Schiff bases; antimicrobial evaluation against multidrug resistant strains and molecular docking

et al. 2019

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Background 2-Aminothiazoles are significant class of organic medicinal compounds utilized as starting material for the synthesis of diverse range of heterocyclic analogues with promising therapeutic roles as antibacterial, antifungal, anti-HIV, antioxidant, antitumor, anthelmintic, anti-inflammatory & analgesic agents. Experimental Eight compounds 1a, 2a–2g were synthesized and characterized by FTIR and NMR (1H and 13C). Evaluation of antibacterial potential against multi-drug resistant clinical isolates was performed and minimum inhibitory concentration (MIC) values were determined. Antifungal activity was also performed. Protein–ligand interactions of compounds with target enzyme were evaluated through docking studies. Results Resistance profiling of bacterical clinical isolates (MDRs) depicted that some standard drugs used were not active against these MDRs while our synthesized compounds showed good MIC values. Among all the synthesized compounds, 2a and 2b showed significant antibacterial potential towards gram-positive Staphylococcus epidermidis and gram-negative Pseudomonas aeruginosa at MIC 250 µg/mL and 375 µg/mL respectively. Likewise, compound 2d and 2g exhibited inhibitory potential against gram-positive Staphylococcus aureus and gram-negative Escherichia coli at MIC values of 250 and 375 µg/mL respectively. Compound 2b showed maximum antifungal potential against Candida glabrata (ATCC 62934) with a zone of inhibition 21.0 mm as compared to the reference drug nystatin which showed lesser antifungal potential with a zone of inhibition of 19.1 mm. Candida albicans (ATCC 60387) showed maximum sensitivity to compound 2a with a zone of inhibition 20.0 mm. Its antifungal activity is more in comparison to reference drug nystatin with exhibited the zone of inhibition of 19.3 mm. Designed compounds were docked with the target enzyme UDP-N-acetylmuramate/l-alanine ligase. The compound 2b showed highest binding affinity (− 7.6 kcal/mol). Conclusions The synthesized compounds showed moderate to significant antibacterial and antifungal potential. It is clear from the binding affinities that compounds having hydroxyl group substituted on benzene ring possess strong binding affinity as compared to other analogues. These designed compounds could be considered to act as antagonists against target UDP-N-acetylmuramate/l-alanine ligase.

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Polyelectrolyte Multicomponent Colloidosomes Loaded with Nisin Z for Enhanced Antimicrobial Activity against Foodborne Resistant Pathogens

Cited by 52 publications

References 58 publications

Biodegradable film development by nisin Z addition into hydroxypropylmethylcellulose matrix for mozzarella cheese preservation

Biodegradable film development by nisin Z addition into hydroxypropylmethylcellulose matrix for mozzarella cheese preservation

Antifungal Activity of Chitosan Nanoparticles Encapsulated With Cymbopogon martinii Essential Oil on Plant Pathogenic Fungi Fusarium graminearum

Designing, synthesis and characterization of 2-aminothiazole-4-carboxylate Schiff bases; antimicrobial evaluation against multidrug resistant strains and molecular docking

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