Polyelectrolyte layer assembly of bacterial nanocellulose whiskers with plasmid DNA as biocompatible non-viral gene delivery system

Pötzinger, Yvette; Rabel, Martin; Ahrem, Hannes; Thamm, Jana; Klemm, Dieter; Fischer, Dagmar

doi:10.1007/s10570-018-1664-z

Cited by 23 publications

(17 citation statements)

References 95 publications

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“…Previous studies on nanocellulose‐based materials utilized experiments on cell cultivation, through the growth, propagation and further cell activities to evaluate their biocompatibility. [ 220,229 ] Biocompatible and antioxidant characteristics of longer BC after the functionalization with sulfate groups through acetosulfation have been verified by cell viability, antioxidant, and hemocompatibility assays. [ 230 ] Such sulfated BC was found to be highly blood compatible, because it did not lyse human red blood cells (RBC) and maintained the integrity of cells after treatment.…”

Section: Ionic Nanocellulose‐based Compounds and Their Applications As Biomaterialsmentioning

confidence: 99%

Recent Progress on Cellulose‐Based Ionic Compounds for Biomaterials

Yang

Zeng

et al. 2020

Advanced Materials

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Cellulose and hemicelluloses represent the most abundant biomaterials on the Earth and represent major structural components in cell walls of plant that provide them with sufficient mechanical Glycans play important roles in all major kingdoms of organisms, such as archea, bacteria, fungi, plants, and animals. Cellulose, the most abundant polysaccharide on the Earth, plays a predominant role for mechanical stability in plants, and finds a plethora of applications by humans. Beyond traditional use, biomedical application of cellulose becomes feasible with advances of soluble cellulose derivatives with diverse functional moieties along the backbone and modified nanocellulose with versatile functional groups on the surface due to the native features of cellulose as both cellulose chains and supramolecular ordered domains as extractable nanocellulose. With the focus on ionic cellulose-based compounds involving both these groups primarily for biomedical applications, a brief introduction about glycoscience and especially native biologically active glycosaminoglycans with specific biomedical application areas on humans is given, which inspires further development of bioactive compounds from glycans. Then, both polymeric cellulose derivatives and nanocellulose-based compounds synthesized as versatile biomaterials for a large variety of biomedical applications, such as for wound dressings, controlled release, encapsulation of cells and enzymes, and tissue engineering, are separately described, regarding the diverse routes of synthesis and the established and suggested applications for these highly interesting materials.

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Section: Ionic Nanocellulose‐based Compounds and Their Applications As Biomaterialsmentioning

confidence: 99%

Recent Progress on Cellulose‐Based Ionic Compounds for Biomaterials

Yang

Zeng

et al. 2020

Advanced Materials

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“…Recently, several studies have reported that polyelectrolytes enhanced the cells membrane permeability, and also increased the efficacy of gene transfer and siRNA delivery. [10][11][12][13] So polyelectrolytes have been widely used as a coating material for carriers. In the present study, cationic/anionic polyelectrolyte coated VPGs loaded with Ara-C were prepared to enhance both in vitro and in vivo anti-glioma effects.…”

Section: Discussionmentioning

confidence: 99%

“…8,9 Recent studies have shown that polyelectrolytes increase the permeability of cell membranes, improving siRNA delivery and enhancing the efficacy of gene transfer. [10][11][12][13] Some studies show that the surface properties of polymer-modified tumor targeting carriers enhance anti-tumor effects. 14 Usually, polyelectrolyte modification can change the original properties of carriers, and polyelectrolyte modified carriers with cationic/anionic changes produce specific electrostatic interactions with the surface charge of cancer cells.…”

Section: Introductionmentioning

confidence: 99%

<p>Cationic/Anionic Polyelectrolyte (PLL/PGA) Coated Vesicular Phospholipid Gels (VPGs) Loaded with Cytarabine for Sustained Release and Anti-glioma Effects</p>

Qi¹,

Zhang²,

Tang³

et al. 2020

DDDT

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Background: Cationic and anionic polymer-modified nanoparticles offer promising properties for the drug and gene delivery. Our study uses cationic/anionic polyelectrolyte coated vesicular phospholipid gels (VPGs) loaded with cytarabine (Ara-C) that enhance in vitro and in vivo anti-glioma effects. Methods: Sodium cholesteryl sulfate (SCS) or octadecylamine (ODA) incorporated in a phospholipids phase were used to prepare charged VPGs, and cationic ε-polylysine (PLL) coated VPGs (PLL-SCS VPGs) and anionic γ-polyglutamic acid (PGA) coated VPGs (PGA-ODA VPGs) were prepared via electrostatic interactions, respectively. The morphology, particle size, zeta potential, rheology properties, and in vitro release were then characterized. The in vitro cytotoxicity and cellular uptake were evaluated on U87-MG glioma cells. The in vivo antitumor effects were studied on BALB/c nude mice bearing a right flank U87-MG glioma model. Results: The TEM images and physicochemical properties of cationic/anionic polyelectrolyte coated VPGs exhibited that polymers covered on the vesicular surface. The results of rheologic property analysis showed that cationic/anionic polyelectrolyte coated VPGs enhanced the viscosity of uncoated VPGs. The in vitro release experiments revealed that cationic/anionic polyelectrolyte coated VPGs kept Ara-C sustained release up to 18 days. Specially, compared with PLL-SCS VPGs, PGA-ODA VPGs demonstrated higher in vitro cytotoxicity and cellular uptake efficiency in U87-MG glioma cells, and enhanced in vivo antitumor effects when subcutaneously injected around the tumor. No severe toxicity appeared in the right flank U87-MG glioma model of BALB/c nude mice. Conclusion: Anionic γ-PGA coated VPGs were superior to cationic PLL coated VPGs in terms of improving the anti-glioma effect for local delivery.

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“…Significant differences at 48 and 72 h were indicated by stars; ∗∗: p-value < 1 %, ∗∗∗: p-value < 0,1 %, n = 3 [ 45 ]; (c) CLSM images of DTAF stained PsCNCs (green fluorescence) treated SKOV3 cells and untreated cells. Nucleus stained with Hoechst-33342 (blue fluorescence) [ 179 ]; (d) Schematic presentation of loading of DNA on nanocellulose [ 183 ]; (e) Viability and transfection efficiency of NIH3T3 cells treated with various concentration of DCC (left) and DCC-RGD (right) for 48 h [ 184 ]. …”

Section: Delivery Of Active Molecules/therapeutic Agentsmentioning

confidence: 99%

Nanocellulose, a versatile platform: From the delivery of active molecules to tissue engineering applications

Patil

Patel

Dutta

et al. 2022

Bioactive Materials

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Nanocellulose, a biopolymer, has received wide attention from researchers owing to its superior physicochemical properties, such as high mechanical strength, low density, biodegradability, and biocompatibility. Nanocellulose can be extracted from wide range of sources, including plants, bacteria, and algae. Depending on the extraction process and dimensions (diameter and length), they are categorized into three main types: cellulose nanocrystals (CNCs), cellulose nanofibrils (CNFs), and bacterial nanocellulose (BNC). CNCs are a highly crystalline and needle-like structure, whereas CNFs have both amorphous and crystalline regions in their network. BNC is the purest form of nanocellulose. The nanocellulose properties can be tuned by chemical functionalization, which increases its applicability in biomedical applications. This review highlights the fabrication of different surface-modified nanocellulose to deliver active molecules, such as drugs, proteins, and plasmids. Nanocellulose-mediated delivery of active molecules is profoundly affected by its topographical structure and the interaction between the loaded molecules and nanocellulose. The applications of nanocellulose and its composites in tissue engineering have been discussed. Finally, the review is concluded with further opportunities and challenges in nanocellulose-mediated delivery of active molecules.

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Polyelectrolyte layer assembly of bacterial nanocellulose whiskers with plasmid DNA as biocompatible non-viral gene delivery system

Cited by 23 publications

References 95 publications

Recent Progress on Cellulose‐Based Ionic Compounds for Biomaterials

Recent Progress on Cellulose‐Based Ionic Compounds for Biomaterials

<p>Cationic/Anionic Polyelectrolyte (PLL/PGA) Coated Vesicular Phospholipid Gels (VPGs) Loaded with Cytarabine for Sustained Release and Anti-glioma Effects</p>

Nanocellulose, a versatile platform: From the delivery of active molecules to tissue engineering applications

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