Polyelectrolyte complex formation using alginate and chitosan

Sæther, Heidi Vogt; Holme, Hilde K.; Maurstad, Gjertrud; Smidsrød, Olav; Stokke, Bjørn Torger

doi:10.1016/j.carbpol.2008.04.048

Cited by 305 publications

(167 citation statements)

References 36 publications

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“…The complex formation from electrostatic interaction between negatively charged amino acids of GFP and positively charged amino acids of Tat or V was expected. Since the reduction in particle sizes from the dense and homogeneous arrangement between molecules during the complex formation has been reported (Saether et al, 2008;Gorajana et al, 2010), then the reduction of hydrodynamic particle sizes of the mixtures in comparing with the single protein or peptides indicated the interaction between GFP and peptides. Particle sizes of GFP, Tat, and V, as determined by dynamic light scattering (DLS), were 421.50 ± 83.79, 272.60 ± 61.38, and 451.80 ± 78.76 nm, respectively, whereas the particle sizes of Tat-GFP (1:1 molar ratio), V-GFP (1:1 molar ratio), and V-Tat-GFP (1:1:1 molar ratio) were reduced to 192.60 ± 41.13, 140.00 ± 8.71, and 226.00 ± 50.72 nm, respectively.…”

Section: Discussionmentioning

confidence: 93%

Polioviral receptor binding ligand: A novel and safe peptide drug carrier from polioviral capsid

Manosroi¹,

Lohcharoenkal²,

Götz³

et al. 2011

Drug Delivery

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Section: Discussionmentioning

confidence: 93%

Polioviral receptor binding ligand: A novel and safe peptide drug carrier from polioviral capsid

Manosroi¹,

Lohcharoenkal²,

Götz³

et al. 2011

Drug Delivery

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“…SA solution is then slowly added to CH solution at a flow rate of 1 ml/s with the help of high-speed homogenizer (Unidrive X1000D Homogenizer drive, CAT scientific laboratory, California) at 10,000 rpm. Alginate in CH PECs was thus formed [10].…”

Section: Preparation Of Pec Nanoparticles Of Alginate and Chmentioning

confidence: 99%

“…Various types of nanoparticles have been studied earlier [7,8]. Polyelectrolyte complexation (PEC) method is selected for preparation of nanoparticles as these are considered as the emerging delivery systems for the oral delivery of proteins, nucleic acids [9,10], etc. A combination of chitosan (CH) and alginate was used as the polyelectrolyte combination.…”

Section: Introductionmentioning

confidence: 99%

Novel Nanoparticles for the Oral Delivery of Low Molecular Weight Heparin: In Vitro and in Vivo Assessment

Nallaguntla

Muzib

Aukunuru³

et al. 2017

Asian J Pharm Clin Res

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Objective: The objective of the present study was to prepare and evaluate a novel oral formulation of nanoparticles for the systemic delivery of lowmolecular-weight heparin (LMWH).Methods: Nanoparticles were prepared by polyelectrolyte complexation method using polymers, i.e., sodium alginate and chitosan (CH). Fourier transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), X-ray diffraction (XRD), entrapment efficiency, In vitro release and scanning electron microscopic studies were carried out for nanoparticles. Ex vivo permeation studies were performed with optimized formulation using small intestine of rat and in vivo studies were conducted on rat model. Results:Entrapment efficiency of LMWH in nanoparticles was found to be 88%. In vitro release studies demonstrated that the release of LMWH was negligible in the stomach and high in the small intestine. FTIR has indicated that there is no interaction between the ingredients in nanoparticle. DSC and XRD studies confirmed that the amino groups of CH interacted with the carboxylic groups of alginate. In vitro % drug release of 95% was shown by formulation AC5. Ex vivo permeation studies have elucidated that ~73% of LMWH was transported across the epithelium. Nanoparticles have shown enhanced oral bioavailability of LMWH as revealed by 4.5-fold increase in area under the curve of plasma drug concentration-time curve. Conclusion:The results suggest that the nanoparticles prepared can result in targeted delivery of LMWH into systemic circulation through intestinal and colon routes. Novel nanoparticles thus prepared in this study can be considered as a promising delivery system.

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“…They result from an electrostatic binding between oppositely charged chains, with counterions release (Michaels et al, 1965;Sukhorukov et al, 1998;Petrov et al, 2003;Dautzenberg and Kriz, 2003;Schatz et al, 2004;Ga¨rdlund et al, 2007;Saether et al, 2008). PEC can be composed of natural and/or synthetic polyions, charged surfactants, etc.…”

Section: Introductionmentioning

confidence: 99%

Formation of Soft NanoparticlesviaPolyelectrolyte Complexation: A Viscometric Study

Rondon

Argillier

Moan

et al. 2014

Oil Gas Sci. Technol. – Rev. IFP Energies nouvelles

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Nous avons aussi mesure´la viscosite´relative des suspensions; la fraction volumique des particules a pu eˆtre estime´e en faisant l'hypothe`se que la contribution des particules et du polycation en exce`s sont inde´pendantes. Nous avons pu e´tablir l'e´volution de la stoechiome´trie des PEC et de leur fraction volumique en fonction du ratio PAH/PSS dans le me´lange initial et du pH. Nos re´sultats montrent que l'accessibilite´des charges (conformation des chaines) de´termine a`la fois la stoechiome´trie et le niveau de compaction des complexes.Abstract -Formation of Soft Nanoparticles via Polyelectrolyte Complexation: A Viscometric Study -This paper describes the formation of soft nanoparticles resulting from electrostatically driven complexation of oppositely charged polyelectrolytes. The system was composed of a strong polyanion (PolyStyrene Sulfonate, PSS) and a weak polycation (Poly(Allylamine) Hydrochloride, PAH) in large excess. Soft nanoparticles were obtained by pouring a PSS solution into a PAH one under constant stirring. The PolyElectrolyte Complexes (PEC) were characterized through a viscometric study complemented by Dynamic Light Scattering (DLS), electrophoretic mobility and suspension turbidity measurements. PEC suspensions were centrifuged and by measuring the viscosity of the supernatant, we were able to estimate the free polycation concentration and hence the percentage of complexed polycation. We also measured the relative viscosity of the suspensions; from the estimated contribution of the PEC particles and of the polycation in excess, the average particle volume fraction was estimated. From all viscometric data, we could derive the evolution of the binding stoichiometry in PEC and of the effective particle volume fraction as a function of the mixing ratio (ratio of the cationic to anionic groups) and of the pH. Our results emphasize the importance of charge accessibility in controlling both the stoichiometry and packing density of the complexes.

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Polyelectrolyte complex formation using alginate and chitosan

Cited by 305 publications

References 36 publications

Polioviral receptor binding ligand: A novel and safe peptide drug carrier from polioviral capsid

Polioviral receptor binding ligand: A novel and safe peptide drug carrier from polioviral capsid

Novel Nanoparticles for the Oral Delivery of Low Molecular Weight Heparin: In Vitro and in Vivo Assessment

Formation of Soft NanoparticlesviaPolyelectrolyte Complexation: A Viscometric Study

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