Polydopamine Nanotubes as an Effective Fluorescent Quencher for Highly Sensitive and Selective Detection of Biomolecules Assisted with Exonuclease III Amplification

Abstract

In this work, the effective fluorescence quenching ability of polydopamine nanotubes (PDANTs) toward various fluorescent dyes was studied and further applied to fluorescent biosensing for the first time. The PDANTs could quench the fluorophores with different emission frequencies, aminomethylcoumarin acetate (AMCA), 6-carboxyfluorescein (FAM), 6-carboxytetramethylrhodamine (TAMRA), and Cy5. All the quenching efficiencies reached to more than 97%. Taking advantage of PDANTs' different affinities toward ssDNA and dsDNA and utilizing the complex of FAM-labeled ssDNA and PDANTs as a sensing platform, we achieved highly sensitive and selective detection of human immunodeficiency virus (HIV) DNA and adenosine triphosphate (ATP) assisted with Exonuclease III amplification. The limits of detection (LODs) of HIV DNA and ATP reached to 3.5 pM and 150 nM, respectively, which were all lower than that of previous nanoquenchers with Exo III amplification, and the platform also presented good applicability in biological samples. Fluorescent sensing applications of this nanotube enlightened other targets detection based upon it and enriched the building blocks of fluorescent sensing platforms. This polydopamine nanotube also possesses excellent biocompatibility and biodegradability, which is suitable for future drug delivery, cell imaging, and other biological applications.

Size‐tunable two‐dimensional Pd@Au nanoplates as a platform for fluorescence sensing

“…Taking into account the advantages of functional nucleic acids (e.g., aptamer), such as sufficient affinity and specificity toward nucleic acids, proteins, and other molecular targets, the combination of Pd@Au nanoplates and the aptamer could be utilized as a universal platform for sensing related analytes . As a proof of concept, an anti‐adenine aptamer sequence and a T‐rich mercury‐specific oligonucleotide (MSO) were introduced to analyze adenosine triphosphate (ATP) and mercuric ion (Hg 2+ ), respectively . For the assay of ATP, the scheme of the aptamer‐based platform was similar to that for the assay of DNA.…”

Polydopamine Based Colloidal Materials: Synthesis and Applications

“…The obtained polydopamine nanotubes were superior mass‐type sensing materials, exhibiting a good selectivity, sensitivity and repeatability to detect formaldehyde . Dong and coworkers followed the same way to prepared polydopamine nanotubes, but the products were used for effectively fluorescent quenching of various fluorescent dyes …”

Tyramine Hydrochloride Based Label‐Free System for Operating Various DNA Logic Gates and a DNA Caliper for Base Number Measurements

“…[11] Many DNA logic circuits have been reported to mimic basic, advanced, or even concatenatedl ogic computation. [11][12][13][14][15][16][17][18][19][20] Additionally,t he DNA system,w hich can not only operate logic computation, but also perform bioanalysis, is ap romising prospect for the combination of logic intelligence [21] with analysis [22,23] in the future. According to the output strategies, DNA logic gates can be divided into two kinds:l abeled and unlabeled ones.C ompared with the labeled strategy,w hich requires expensive chemical labels, unlabeledr eporter based DNA logic gates have been broadly constructed [15,16] as ar esult of their low cost and simple operation.…”

“…Alone T-HCl is nonfluorescent, but exhibits dramatically enhanced fluorescencea fter oxidized by H 2 O 2 under the catalysis of G4zyme (Scheme1). [23] Caie tal. designed as ensitives ensor for Cu 2 + by takinga dvantage of this property of T-HCl.…”