Polydopamine-mediated covalent functionalization of collagen on a titanium alloy to promote biocompatibility with soft tissues

Zhu, Yi; Liu, Dandan; Wang, Xiuli; He, Yang; Luan, Wenjie; Qi, Fazhi; Ding, Jiandong

doi:10.1039/c8tb03379j

Cited by 48 publications

(36 citation statements)

References 74 publications

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“…Recently, Zhu et al. [38] modified a Ti percutaneous implant by first depositing a PDA coating on Ti6Al4V based on dopamine self-polymerization and then immobilizing collagen chains on PDA. This modification could improve the integration between the implant and soft tissues.…”

Section: Characteristics Of Pda-modified Materialsmentioning

confidence: 99%

Polydopamine-assisted surface modification for orthopaedic implants

Jia

Han

Wang

et al. 2019

Journal of Orthopaedic Translation

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Along with the massive use of implants in orthopaedic surgeries in recent few decades, there has been a tremendous demand for the surface modification of the implants to avoid surgery failure and improve their function. Polydopamine (PDA), being able to adhere to almost all kinds of substrates and possessing copious functional groups for covalently immobilizing biomolecules and anchoring metal ions, has been widely used for surface modification of materials since its discovery in the last decade. PDA and its derivatives can be used for the surface modification of orthopaedic implants to modulate cellular responses, including cell spreading, migration, proliferation, and differentiation, and may thereby enhance the function of existing implants. In addition, the osseointegration and antimicrobial properties of orthopaedic implants may also be improved by PDA-based coatings. The aim of this review is to provide a brief overview of current advances of surface modification technologies for orthopaedic implants using PDA and its derivatives as a medium. Given the versatility of PDA-based adhesion, such PDA-assisted surface modification technologies will certainly benefit the development of new orthopaedic implants. The translational potential of this article Surface treatments of orthopaedic implants, which are normally inert materials, are essential for their performance in vivo. This review summarizes recent advances in the surface modification of orthopaedic implants using facile and highly versatile techniques based on the use of polydopamine (PDA) and its derivatives.

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Section: Characteristics Of Pda-modified Materialsmentioning

confidence: 99%

Polydopamine-assisted surface modification for orthopaedic implants

Jia

Han

Wang

et al. 2019

Journal of Orthopaedic Translation

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“…Without oxygen plasma treatment, the PDMS surface remains hydrophobic and does not allow the cells to adhere and proliferate either [10]. Generally, ECM proteins enhance cell attachment and proliferation by interacting via integrins or other cell adhesion molecules presented on a cell's membrane [11][12][13][14][15]. This means that cell receptors recognize the sequence of polypeptide chains in collagen molecules [16].…”

Section: Introductionmentioning

confidence: 99%

Surface Modification of PDMS-Based Microfluidic Devices with Collagen Using Polydopamine as a Spacer to Enhance Primary Human Bronchial Epithelial Cell Adhesion

et al. 2021

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Polydimethylsiloxane (PDMS) is a silicone-based synthetic material used in various biomedical applications due to its properties, including transparency, flexibility, permeability to gases, and ease of use. Though PDMS facilitates and assists the fabrication of complicated geometries at micro- and nano-scales, it does not optimally interact with cells for adherence and proliferation. Various strategies have been proposed to render PDMS to enhance cell attachment. The majority of these surface modification techniques have been offered for a static cell culture system. However, dynamic cell culture systems such as organ-on-a-chip devices are demanding platforms that recapitulate a living tissue microenvironment’s complexity. In organ-on-a-chip platforms, PDMS surfaces are usually coated by extracellular matrix (ECM) proteins, which occur as a result of a physical and weak bonding between PDMS and ECM proteins, and this binding can be degraded when it is exposed to shear stresses. This work reports static and dynamic coating methods to covalently bind collagen within a PDMS-based microfluidic device using polydopamine (PDA). These coating methods were evaluated using water contact angle measurement and atomic force microscopy (AFM) to optimize coating conditions. The biocompatibility of collagen-coated PDMS devices was assessed by culturing primary human bronchial epithelial cells (HBECs) in microfluidic devices. It was shown that both PDA coating methods could be used to bind collagen, thereby improving cell adhesion (approximately three times higher) without showing any discernible difference in cell attachment between these two methods. These results suggested that such a surface modification can help coat extracellular matrix protein onto PDMS-based microfluidic devices.

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“…Most of the stratified scaffolds were fabricated by composite biomaterials for repair of the osteochondral defect [7], [21]. Although certainly an incorporation of natural extracellular matrix (ECM) into biomaterial scaffolds can promote repair of cartilage and soft tissues [34], [35], [36], [37], we used, in this study, merely synthetic PLGA to fabricate the integrated bilayered scaffold, which was made of the same raw material yet had different pore sizes between the chondral layer and subchondral layer to mimic the different mechanical properties of cartilage and subchondral bone. From the results of this study, PLGA bilayered scaffolds seeded with BMSCs in the chondral layer supported the simultaneous regeneration of cartilage and subchondral bone in rabbits.…”

Section: Discussionmentioning

confidence: 99%

Restoration of osteochondral defects by implanting bilayered poly(lactide-co-glycolide) porous scaffolds in rabbit joints for 12 and 24 weeks

Duan

Zhang

Cao

et al. 2019

Journal of Orthopaedic Translation

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BackgroundWith the ageing of the population and the increase of sports injuries, the number of joint injuries has increased greatly. Tissue engineering or tissue regeneration is an important method to repair articular cartilage defects. While it has recently been paid much attention to use bilayered porous scaffolds to repair both cartilage and subchondral bone, it is interesting to examine to what extent a bilayer scaffold composed of the same kind of the biodegradable polymer poly(lactide-co-glycolide) (PLGA) can restore an osteochondral defect. Herein, we fabricated bilayered PLGA scaffolds and used a rabbit model to examine the efficacy of implanting the porous scaffolds with or without bone marrow mesenchymal stem cells (BMSCs). The present manuscript reports the regenerative potential up to 24 weeks.MethodsThe osteochondral defect, 4 mm in diameter and 5 mm in depth, was created in the medial condyle of each knee in 23 rabbits. The bilayered PLGA scaffolds with a pore size of 100–200 μm in the chondral layer and a pore size of 300–450 μm in the osseous layer, seeded with or without BMSCs in the chondral layer, were then transplanted into the osteochondral defect of each knee. The osteochondral defect created in the same manner was untreated to act as the control. At 12 and 24 weeks postoperatively, condyles were harvested and analyzed using histology, immunohistochemistry, real-time polymerase chain reaction, and biomechanical testing to evaluate the efficacy of osteochondral repair.ResultsNo joint erosion, inflammation, swelling, or deformity was observed, and all animals maintained a full range of motion. Compared with the untreated blank group, the groups implanting the bilayered scaffolds with or without cells exhibited much better resurfacing, similar to the surrounding normal tissue. The histological scores of neotissues repaired by the scaffold with cells were closer to that of normal tissue. Although the biomechanical properties of neotissues were not as good as the normal tissue, no significant difference was found between the gene levels of neotissues repaired by the scaffold with or without cells and that of the normal tissue. The repair of the osteochondral defect tends to be stable 12 weeks after implantation.ConclusionsOur bilayered PLGA porous scaffold supports long-term osteochondral repair via in vivo tissue engineering or regeneration, and its effect can be further facilitated under the scaffold seeded with allogenic BMSCs.The translational potential of this articleThe bilayered PLGA porous scaffold can facilitate the repair of osteochondral defects and has potential for application in osteochondral tissue engineering.

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Polydopamine-mediated covalent functionalization of collagen on a titanium alloy to promote biocompatibility with soft tissues

Abstract: A facile method to achieve a stable collagen coating on a titanium alloy was put forward to promote the integration between a percutaneous implant and soft tissue.

Cited by 48 publications

References 74 publications

Polydopamine-assisted surface modification for orthopaedic implants

Polydopamine-assisted surface modification for orthopaedic implants

Surface Modification of PDMS-Based Microfluidic Devices with Collagen Using Polydopamine as a Spacer to Enhance Primary Human Bronchial Epithelial Cell Adhesion

Restoration of osteochondral defects by implanting bilayered poly(lactide-co-glycolide) porous scaffolds in rabbit joints for 12 and 24 weeks

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