Polydopamine-functionalized monolithic silica column for mixed-mode chromatography†

Abstract

Dopamine is easy to self-polymerize under alkaline conditions and the resultant polydopamine is easy to adhere to the surface of many organic and inorganic materials. Based on the characteristics of dopamine, in this paper, a new polydopamine functionalized monolithic silica column was successfully prepared for performing mixed-mode chromatography. The performance of the column was evaluated by the separation of different types of samples including alkylbenzenes, polycyclic aromatic hydrocarbons, aromatic acids, phenols, and bases. The mechanism for the separation of these compounds was studied and appeared to involve the mixed interactions containing π-π, hydrophobic, electrostatic, and hydrophilic interactions.

Facile silicification of plastic surface for bioassays

“…Following the pDA coating, we grew a biocompatible silica (SiO 2 ) layer, mimicking the silicification process in diatom shell formation. The binding mechanism between pDA and plastics is likely hydrophobic interactions 21 due to the hydrophobic nature of plastics surfaces. The silicification process is the same as occurred in diatoms; i) electrostatic interaction triggering the chemisorption of monosilicic acid on positively charged pDA layer and ii) simultaneous polymerization of surface-bound monosilicic acid into SiO 2 .…”

Histidine-modified organic-silica hybrid monolithic column for mixed-mode per aqueous and ion-exchange capillary electrochromatography

“…In addition, mixed-mode chromatography also has the superiority of high selectivity and high loading capacity [34]. Owing to its distinct advantages, the application of mixed-mode chromatography in CEC has been growing rapidly [35][36][37][38][39]. The development of new monolithic stationary phases for mixed-mode CEC to achieve multiple separation capabilities with one column is of great practical significance.…”

Mixed-mode chromatographic stationary phases: Recent advancements and its applications for high-performance liquid chromatography

“…Recently, monolithic-based mixed-mode stationary phases [73,74] have also been reported, as summarized in Table 5.…”