Polydopamine at biological interfaces

Alfieri, Maria Laura; Weil, Tanja; Ng, David Y. W.; Ball, Vincent

doi:10.1016/j.cis.2022.102689

Cited by 128 publications

(50 citation statements)

References 99 publications

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“…Biomimetic modification of the micro/nano interface is commonly effective to immobilize antibodies. Inspired by the structure of marine mussels, polydopamine (PDA) chemistry has been used as a simple and versatile approach for the (bio) functionalization of materials ( Alfieri et al, 2022 ). On the one hand, PDA coating can improve the hydrophilicity and biocompatibility of materials.…”

Section: Introductionmentioning

confidence: 99%

“…Polydopamine is the product of the self-polymerization of dopamine (DA) under alkaline conditions with air or oxygen as the oxidant. Usually, PDA can form and stably combine on the surface of almost all materials (for example, ceramics, semiconductors, metals, and even synthetic polymers) ( Samyn, 2021 ; Alfieri et al, 2022 ; Prabhu et al, 2022 ). On the other hand, polydopamine has secondary reactivity and can be directly used as the bio-linker for covalent immobilization of recognitive antibodies ( Ding et al, 2016 ; Huang et al, 2020 ; Li et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

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A reagentless electrochemical immunosensor for sensitive detection of carcinoembryonic antigen based on the interface with redox probe-modified electron transfer wires and effectively immobilized antibody

et al. 2022

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Convenient and sensitive detection of tumors marked in serum samples is of great significance for the early diagnosis of cancers. Facile fabrication of reagentless electrochemical immunosensor with efficient sensing interface and high sensitivity is still a challenge. Herein, an electrochemical immunosensor was easily fabricated based on the easy fabrication of immunoassay interface with electron transfer wires, confined redox probes, and conveniently immobilized antibodies, which can achieve sensitive and reagentless determination of the tumor marker, carcinoembryonic antigen (CEA). Carboxyl multi-walled carbon nanotubes (MWCNTs) were firstly modified with an electrochemical redox probe, methylene blue (MB), which has redox potentials distinguished from those of redox molecules commonly existing in biological samples (for example, ascorbic acid and uric acid). After the as-prepared MB-modified MWCNT (MWCNT-MB) was coated on the supporting glassy carbon electrode (GCE), the MWCNT-MB/GCE exhibited improved active area and electron transfer property. Polydopamine (PDA) was then in situ synthesized through simple self-polymerization of dopamine, which acts as the bio-linker to covalently immobilize the anti-CEA antibody (Ab). The developed immunosensor could be applied for electrochemical detection of CEA based on the decrease in the redox signal of MB after specific binding of CEA and immobilized Ab. The fabricated immunosensor can achieve sensitive determination of CEA ranging from 10 pg/ml to 100 ng/ml with a limit of detection (LOD) of 0.6 pg/ml. Determination of CEA in human serum samples was also realized with high accuracy.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A reagentless electrochemical immunosensor for sensitive detection of carcinoembryonic antigen based on the interface with redox probe-modified electron transfer wires and effectively immobilized antibody

et al. 2022

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“…Nevertheless, the immobilization of gelatin on the PDA (PDA-Gel) changes the features of gelatin, such as conformation, amount, and/or bioactivity. Alfieri et al (2022 ) found that proteins attach to the PDA surface both physically and covalently, and the structural changes of proteins are likely to occur ( Giol et al, 2015 ). The gene expression of vinculin and FAK revealed a 50% downregulation on the PDA-Gel substrate compared to the Gel surface ( Figure 2B ).…”

Section: Resultsmentioning

confidence: 99%

Polydopamine-Mediated Protein Adsorption Alters the Epigenetic Status and Differentiation of Primary Human Adipose-Derived Stem Cells (hASCs)

Harati

Tao

Shahsavarani

et al. 2022

Front. Bioeng. Biotechnol.

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Polydopamine (PDA) is a biocompatible cell-adhesive polymer with versatile applications in biomedical devices. Previous studies have shown that PDA coating could improve cell adhesion and differentiation of human mesenchymal stem cells (hMSCs). However, there is still a knowledge gap in the effect of PDA-mediated protein adsorption on the epigenetic status of MSCs. This work used gelatin-coated cell culture surfaces with and without PDA underlayer (Gel and PDA-Gel) to culture and differentiate primary human adipose-derived stem cells (hASCs). The properties of these two substrates were significantly different, which, in combination with a variation in extracellular matrix (ECM) protein bioactivity, regulated cell adhesion and migration. hASCs reduced focal adhesions by downregulating the expression of integrins such as αV, α1, α2, and β1 on the PDA-Gel compared to the Gel substrate. Interestingly, the ratio of H3K27me3 to H3K27me3+H3K4me3 was decreased, but this only occurred for upregulation of AGG and BMP4 genes during chondrogenic differentiation. This result implies that the PDA-Gel surface positively affects the chondrogenic, but not adipogenic and osteogenic, differentiation. In conclusion, for the first time, this study demonstrates the sequential effects of PDA coating on the biophysical property of adsorbed protein and then focal adhesions and differentiation of hMSCs through epigenetic regulation. This study sheds light on PDA-mediated mechanotransduction.

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“…Dopamine is a biological glue extracted from mussels, and the properties of self-polymerization of dopamine in weakly alkaline solutions have enabled researchers to prepare polydopamine (PDA) coatings on a variety of solid substrates for use in environmental, energy, and biomedical applications . With rich catechol groups, dopamine can be used as a biofriendly material for chemical reduction welding.…”

Section: Introductionmentioning

confidence: 99%

Chemically Welding Silver Nanowires toward Transferable and Flexible Transparent Electrodes in Heaters and Double-Sided Perovskite Solar Cells

Bian

Qian

Cao

et al. 2023

ACS Appl. Mater. Interfaces

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Silver nanowires (AgNWs) are important materials for flexible transparent electrodes (FTEs). However, the loose stacking of nanowire junctions greatly affects the electric conductivity across adjacent nanowires. Soldering can effectively reduce the wire−wire contact resistance of AgNWs by epitaxially depositing nanosolders at the junctions, but the process normally needs to be performed with high energy consumption. In this work, we proposed a simple room-temperature method to achieve precise welding of junctions by adjusting the wettability of the soldered precursor solution on the surfaces of AgNWs. The nanoscale welding at nanowire cross junctions forms efficient conductive networks. Furthermore, reduced graphene oxide (rGO) was used to improve the stability of FTEs by wrapping the rGO around the AgNW surface. The obtained FTE shows a figure-of-merit (FoM) of up to 439.3 (6.5 Ω/sq at a transmittance of 88%) and has significant bending stability and environmental and acidic stability. A flexible transparent heater was successfully constructed, which could reach up to 160 °C within a short response time (43 s) and exhibit excellent switching stability. When laminating this FTE onto half perovskite solar cells as the top electrodes, the obtained double-side devices achieved power conversion efficiencies as high as 16.15% and 13.91% from each side, pointing out a convenient method for fabricating double-sided photovoltaic devices.

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Polydopamine at biological interfaces

Cited by 128 publications

References 99 publications

A reagentless electrochemical immunosensor for sensitive detection of carcinoembryonic antigen based on the interface with redox probe-modified electron transfer wires and effectively immobilized antibody

A reagentless electrochemical immunosensor for sensitive detection of carcinoembryonic antigen based on the interface with redox probe-modified electron transfer wires and effectively immobilized antibody

Polydopamine-Mediated Protein Adsorption Alters the Epigenetic Status and Differentiation of Primary Human Adipose-Derived Stem Cells (hASCs)

Chemically Welding Silver Nanowires toward Transferable and Flexible Transparent Electrodes in Heaters and Double-Sided Perovskite Solar Cells

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