Polydopamine assisted preparation of Ti<sup>4+</sup>‐decorated magnetic particles for selective and rapid adsorption of phosphorylated proteins

Ma, Xiangdong; Li, Jia

doi:10.1002/jctb.4654

Cited by 13 publications

(10 citation statements)

References 28 publications

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“…As illustrated in Figure A, Ti 4+ ‐IMAC has higher adsorption capacity for β‐casein than BSA; the saturated adsorption capacity for β‐casein (925.9 mg/g) is more than 15‐fold higher than that of BSA (60.3 mg/g), which demonstrates the excellent phosphoprotein enrichment ability of Ti 4+ ‐IMAC. Comparing with similar phosphoprotein enrichment materials, the adsorption capacity for β‐casein of Ti 4+ ‐IMAC is nearly the same as that of materials published before and larger than that of Ti 4+ ‐immobilized affinity silica nanoparticles . What's more, the Langmuir model was well fitted for the adsorption behaviors of these proteins under the adopted concentration range (Figure B).…”

Section: Resultssupporting

confidence: 54%

“…Jia et al. synthesized and applied the polydopamine assisted Ti 4+ ‐decorated magnetic particles for selective enrichment caseins (α‐, β‐, and κ‐) out of 10‐fold diluted non‐fat milk, and a β‐casein adsorption capacity of 1273.9 mg/g was measured . Yang et al.…”

Section: Introductionmentioning

confidence: 99%

“…In research and development of new IMAC materials for efficient enrichment of intact phosphoproteins, Wei et al prepared a novel phosphate-Zr 4+ immobilized metal affinity membrane for the selective enrichment of β-casein and OVA out of their mixture with BSA and lysozyme as well as OVA out of chicken egg white [25]. Jia et al synthesized and applied the polydopamine assisted Ti 4+ -decorated magnetic particles for selective enrichment caseins (α-, β-, and κ-) out of 10-fold diluted non-fat milk, and a β-casein adsorption capacity of 1273.9 mg/g was measured [26]. Yang et al synthesized aluminium (Al 3+ ) glycinate functionalized silica nanoparticles with a β-casein binding capacity of 1060 mg/g, and enrichment performance in 10-fold diluted drinking milk was also evaluated [27].…”

Section: Introductionmentioning

confidence: 99%

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Enrichment of intact phosphoproteins using immobilized titanium(IV) affinity chromatography microspheres

Wang

Zhong

Xiao

et al. 2018

Separation Science Plus

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Approximately a third of eukaryotic proteins are estimated to be phosphorylated, and the protein phosphorylation is significant in regulating almost all aspects of cell life. Due to low stoichiometry of phosphorylation, efficient enrichment is often an essential step in phosphoproteomics study. Over the decades, lots of enrichment materials have been successfully developed and widely used for intact phosphoproeins. One of these materials is immobilized titanium(IV) affinity chromatography (Ti4+‐IMAC) microspheres, which has been widely studied and used for phosphopeptides enrichment. Application of Ti4+‐IMAC for intact phosphoprotein enrichment has also proven possible, where intact phosphoproteins (α‐casein and β‐casein) were successfully enrichment out of their mixture with BSA (1:1:10, w/w). Here we report our detailed investigation of intact phosphoprotein enrichment using Ti4+‐IMAC. With BSA and standard intact phosphoprotein β‐casein, selectivity with a wide range of mixing ratios of 1:1, 1:100, 1:500, 1:1000 and 1:2000 (β‐casein:BSA, w/w) and binding capacity were investigated; the enrichment capability of Ti4+‐IMAC was further investigated with real biological systems, including non‐fat milk, egg white and swine liver tissue extract. The results show that Ti4+‐IMAC is a promising material for intact phosphoprotein enrichment.

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Section: Resultssupporting

confidence: 54%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Enrichment of intact phosphoproteins using immobilized titanium(IV) affinity chromatography microspheres

Wang

Zhong

Xiao

et al. 2018

Separation Science Plus

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“…Jia et al. synthesized Ti 4+ ‐decorated magnetic particles with polydopamine as linkers and demonstrated phosphoprotein enrichment from nonfat milk . Wang et al.…”

Section: Introductionmentioning

confidence: 99%

“…For example, Lin et al synthesized the Ti 4+ -immobilized affinity silica nanoparticles for intact phosphoprotein enrichment with the adsorption capacity of 328.9 mg/g for β-casein, where γ-methacryloxypropyltrimethoxysilane and vinylphosphonic acid were used as the linkers, then applied SiO 2 -Ti NPs in standard protein mixtures (β-casein/BSA/lysozyme, 3:1:1, w/w) and diluted drinking milk successfully [12]. Jia et al synthesized Ti 4+ -decorated magnetic particles with polydopamine as linkers and demonstrated phosphoprotein enrichment from nonfat milk [13]. Wang et al chose poly(ethylene glycol methacrylate phosphate) as the linker in preparation of the Ti 4+ -immobilized monodisperse microgel for phosphoprotein enrichment in drinking milk [14].…”

Section: Introductionmentioning

confidence: 99%

Facile synthesis of titanium(IV) ion‐immobilized poly‐glycidyl methacrylate microparticles functionalized with polyethylenimine and adenosine triphosphate for highly specific enrichment of intact phosphoproteins

Wang

Tian

2018

J of Separation Science

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In this study, a facile synthesis of the titanium(IV) ion‐immobilized poly‐glycidyl methacrylate microparticles functionalized with polyethylenimine and adenosine triphosphate was developed for efficient enrichment of intact phosphoproteins. The titanium(IV) ion‐immobilized microparticles had higher saturated adsorption capacity for phosphoproteins (1217.6 mg/g for β‐casein) than nonphosphoproteins (97.1 mg/g for bovine serum albumin) and the average particle diameter was about 1.4 μm with good dispersibility. In application, as demonstrated by SDS‐PAGE, titanium(IV) ion‐immobilized microparticles exhibited good performance in enriching intact phosphoproteins from standard protein mixtures of β‐casein and bovine serum albumin with high specificity and selectivity. In addition, titanium(IV) ion‐immobilized microparticles were also successfully applied in intact phosphoprotein enrichment from complex biological samples including nonfat milk, chicken egg white, and mouse heart tissue extract.

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Versatile Surface Modification Using Polydopamine and Related Polycatecholamines: Chemistry, Structure, and Applications

Barclay

Hegab

Clarke

et al. 2017

Adv Materials Inter

291

223

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Almost ten years ago, Lee et al. [13] formulated a universal adhesive coating based on observation of the strong attachment by mussels through their byssal threads to virtually all types of inorganic and organic surfaces. The amino acid compositions of the mussel foot proteins that generate the attachment are rich in catechol and amine groups. [13][14][15] These groups associate under marine conditions, forming strong covalent and noncovalent interactions with substrates. [13] This led to the idea that both catechol and amine groups were crucial in forming robust, wide spectrum adhesive nanolayers [16,17] and consequently dopamine was conceived as a powerful building block for spontaneous deposition of thin polymer films. The dopamine monomer is deposited onto unadulterated surfaces through self-assembly and oxidative cross-linking from mild pH aqueous solution in a rapid reaction. This results in a durable, nanoscale, conformal, and hydrophilic coating that can be readily modified to introduce specific surface chemistries for a particular application. [18][19][20][21][22] These bioinspired, polydopamine materials are chemically and structurally indistinguishable from eumelanins found in the human body, [23,24] providing excellent biocompatibility. Additionally, polydopamine has broad electromagnetic absorption and excellent photothermal energy conversion [25] as well as having ionic-electronic hybrid conductivity. [26] Along with the excellent coating performance, these properties provide a vast array of potential applications for polydopamine materials.In this article, we explain what is known about polydopamine and related catecholamine coatings; their formation, the adhesion mechanism, and their physicochemical properties and we also review the applications of these materials (Figure 1). Since 2011, there have been a number of reviews on this subject matter, including general reviews on the physicochemical properties of polydopamine coatings [11,20,[27][28][29] and their applications. [20,27,30] There are also a number of more specific reviews on the chemical synthesis and modulation of polycatecholamine coatings, [31] the biomedical applications of these coatings, [8,[32][33][34] their electronic properties, [26,35] the use of polydopamine to make films at fluid interfaces, [36] in hierarchically constructed particles, [33] and capsules, [30] exploitation of polycatecholamine coatings in membrane filtration, [37] polydopamine-derived carbon materials, [38] and the use of coordination chemistry in catechol-based materials. [39] Nonetheless, this area of research is growing so rapidly [25,27] that many recent Polydopamine and related polycatecholamines can be easily deposited onto almost any surface from mild, aqueous solution. This results in durable, nanoscale coatings that exhibit high biocompatibility and have useful chemical and electronic properties. Additionally, these materials can be readily chemically and physically modified, and consequently, they are used extensively for surface modification. This ...

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Polydopamine assisted preparation of Ti⁴⁺‐decorated magnetic particles for selective and rapid adsorption of phosphorylated proteins

Cited by 13 publications

References 28 publications

Enrichment of intact phosphoproteins using immobilized titanium(IV) affinity chromatography microspheres

Enrichment of intact phosphoproteins using immobilized titanium(IV) affinity chromatography microspheres

Facile synthesis of titanium(IV) ion‐immobilized poly‐glycidyl methacrylate microparticles functionalized with polyethylenimine and adenosine triphosphate for highly specific enrichment of intact phosphoproteins

Versatile Surface Modification Using Polydopamine and Related Polycatecholamines: Chemistry, Structure, and Applications

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Polydopamine assisted preparation of Ti4+‐decorated magnetic particles for selective and rapid adsorption of phosphorylated proteins

Cited by 13 publications

References 28 publications

Enrichment of intact phosphoproteins using immobilized titanium(IV) affinity chromatography microspheres

Enrichment of intact phosphoproteins using immobilized titanium(IV) affinity chromatography microspheres

Facile synthesis of titanium(IV) ion‐immobilized poly‐glycidyl methacrylate microparticles functionalized with polyethylenimine and adenosine triphosphate for highly specific enrichment of intact phosphoproteins

Versatile Surface Modification Using Polydopamine and Related Polycatecholamines: Chemistry, Structure, and Applications

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Polydopamine assisted preparation of Ti⁴⁺‐decorated magnetic particles for selective and rapid adsorption of phosphorylated proteins