Polydopamine‐assisted immobilization of trypsin onto monolithic structures for protein digestion

Rivera, José G.; Messersmith, Phillip B.

doi:10.1002/jssc.201200073

Cited by 35 publications

(18 citation statements)

References 38 publications

(51 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This method can also be used in various biomedical assays. Recently, the immobilization of trypsin on a silica and titanium support was achieved via a mussel-inspired adhesion strategy [ 26 ]. The method involves the fabrication of titanium substrates with catechol-containing biomimetic PD followed by the fabrication of trypsin on a PD layer.…”

Section: Applications Of Mussel-inspired Materialsmentioning

confidence: 99%

Biomedical and Clinical Importance of Mussel-Inspired Polymers and Materials

Kaushik

Pardeshi

et al. 2015

Marine Drugs

View full text Add to dashboard Cite

The substance secreted by mussels, also known as nature’s glue, is a type of liquid protein that hardens rapidly into a solid water-resistant adhesive material. While in seawater or saline conditions, mussels can adhere to all types of surfaces, sustaining its bonds via mussel adhesive proteins (MAPs), a group of proteins containing 3,4-dihydroxyphenylalanine (DOPA) and catecholic amino acid. Several aspects of this adhesion process have inspired the development of various types of synthetic materials for biomedical applications. Further, there is an urgent need to utilize biologically inspired strategies to develop new biocompatible materials for medical applications. Consequently, many researchers have recently reported bio-inspired techniques and materials that show results similar to or better than those shown by MAPs for a range of medical applications. However, the susceptibility to oxidation of 3,4-dihydroxyphenylalanine poses major challenges with regard to the practical translation of mussel adhesion. In this review, various strategies are discussed to provide an option for DOPA/metal ion chelation and to compensate for the limitations imposed by facile 3,4-dihydroxyphenylalanine autoxidation. We discuss the anti-proliferative, anti-inflammatory, anti-microbial activity, and adhesive behaviors of mussel bio-products and mussel-inspired materials (MIMs) that make them attractive for synthetic adaptation. The development of biologically inspired adhesive interfaces, bioactive mussel products, MIMs, and arising areas of research leading to biomedical applications are considered in this review.

show abstract

Section: Applications Of Mussel-inspired Materialsmentioning

confidence: 99%

Biomedical and Clinical Importance of Mussel-Inspired Polymers and Materials

Kaushik

Pardeshi

et al. 2015

Marine Drugs

View full text Add to dashboard Cite

show abstract

“…It can self-polymerize in alkaline environments to form thin adherent polydopamine (PD) on a wide range of materials including noble metals (e.g., Ag and Au) and iron oxide [13–15]. Polydopamine has also been studied for enzyme immobilization, for example, trypsin [16], glucose oxidase [17], and lipase [18]. Enzymes can be covalently immobilized on a polydopamine surface via nucleophiles [19].…”

Section: Introductionmentioning

confidence: 99%

Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production

Dumri

Anh

2014

Enzyme Research

View full text Add to dashboard Cite

Biodiesel production technology is competitive in terms of low cost and alternative source of energy which should be not only sustainable but also environmentally friendly. Designing of the lipase immobilization for biodiesel production has a remarkable impact and is still challenging. In this work, biodiesel production from soybean oil was enhanced and facilitated by using a novel biocatalyst consisting of commercial lipase (EC 3.1.1.3), silver nanoparticles, and polydopamine. Silver nanoparticles (AgNPs) were synthesized with a size range of 10–20 nm. Polydopamine (PD) was delivered by the self-polymerization of dopamine in 10 mM Tris-HCl pH 8.5 and simultaneously coated the AgNPs to form a PD/AgNPs complex. Lipase was immobilized on the PD/AgNPs complex surface via covalent bonds to form a tailor-made biocatalyst consisting of immobilized lipase/PD/AgNPs complex (LPA). The formation and morphology of each composition were characterized by UV-Vis spectroscopy and scanning electron microscope (SEM). Significantly, gas chromatography analysis showed a remarkable biodiesel production yield of 95% by using the LPA complex at 40°C for 6-hours reaction time, whereas the yield was 86% when using free lyophilized lipase. The LPA complex was apparently reusable after 7 batches and the latter conversion rate of soybean oil was decreased by only 27%.

show abstract

“…Messersmith and Rivera used an immobilized trypsin preparation for the digestion of peptides for proteomic analysis . These were prepared by coating silica and titania‐coated silica monoliths with PDA and then conjugating them with trypsin.…”

Section: Immobilized Enzymes As Catalystsmentioning

confidence: 99%

Polydopamine – its Prolific Use as Catalyst and Support Material

Molnár

2020

ChemCatChem

View full text Add to dashboard Cite

Polydopamine is a mussel-inspired functional material with a unique ability to form surface coatings. It is a green, environmentally benign product available very easily for a multitude of varied applications. This review attempts to collect useful information with respect to polydopamine-based catalytic studies with the use of polydopamine and modified polydopamine preparations loaded with varied catalytically active species including metal ions and metal particles. Major sections are about the use of polydopamine as catalyst material, utilization of immobilized enzymes, removal of toxic materials, results in organic synthesis, and fuel cell applications. A few examples of chiral catalysis and results of recycling studies are also treated. Data collected and analyzed indicate the importance and high potential of polydopamine-based catalysts in sustainable chemistry. of subunits attacked by an immobilized cyclohexeneamine (5) was suggested to be the transition state of CÀ C bond formation.Edouard et al. prepared catalyst 0.29 % PDA/PU by depositing PDA onto polyurethane foam (PU, 180 � 20 mg, 8 cm 3 ) and used it for the reduction of methylene blue (MB) with NaBH 4 . [48] Activities decreased gradually with repeated uses from 100 % to 35 % in run 11 but partial reactivation was achieved upon keeping the sample at 67°C for 8 h (55 % dropping below 10 % in run 13). Exposure to air at room temperature for six days proved to be somewhat more beneficial (90 % conversion in run 14 decreasing to 48 % in run 18). In a recent study they fabricated two oxidized PDA/PU samples and then loaded them with NaBH 4 . [49] One made by the usual method (dopamine/Tris, rt, 12 h, in air; sample PDA O2 PU), whereas the other was made by treating PU in a mixture of dopamine, NaOAc, and NaIO 4 (pH 5, rt, 24 h). The sample was dried (67°C) and washed thoroughly with water (PDA NaIO4 PU). Both products were dipped into an aqueous solution of NaBH 4 (0.1 M, 10 min) to afford boron contents of 2.37 and 2.11 wt%. The catalysts tested in the degradation of MB showed high activities with efficiencies of 97 % and 98 %, respectively (NaBH 4 /MB molar ratio 40, 177 mg and 209 mg catalysts, 25 min). During five reuses, the pH of the reaction medium increased because of boron loss (0.78 wt% and 0.81 wt%). The activity of PDA NaIO4 PU decreased to 89 % in run five. However, dipping the used sample into a 0.1 M NaBH 4 solution restored the original efficiency of 98 %. This, however, was only a temporary remedy, since a low efficiency of 60 % was measured in run 10. PDA was suggested by the authors to act as a redox mediator to prevent the hydrolysis/oxidation of immobilized borohydride.This formula (5) should be moved up and placed before the paragraph starting with "Edouard et al…"The Zuo group used PDA particles (avg. diameter 240 nm) to induce the reduction of MB and rhodamine B (RhB) with NaBH 4 . [50] Quinone moieties of PDA were assumed to channel electrons from NaBH 4 to the dyes. Indeed, PDA oxidized with NaIO 4 exhibited increased degradatio...

show abstract

Polydopamine‐assisted immobilization of trypsin onto monolithic structures for protein digestion

Cited by 35 publications

References 38 publications

Biomedical and Clinical Importance of Mussel-Inspired Polymers and Materials

Biomedical and Clinical Importance of Mussel-Inspired Polymers and Materials

Immobilization of Lipase on Silver Nanoparticles via Adhesive Polydopamine for Biodiesel Production

Polydopamine – its Prolific Use as Catalyst and Support Material

Contact Info

Product

Resources

About