Polydeoxyribonucleotides enhance the proliferation of human skin fibroblasts: Involvement of A2 purinergic receptor subtypes

Thellung, Stefano; Florio, Tullio; Maragliano, Alberto; Cattarini, G.; Schettini, Gennaro

doi:10.1016/s0024-3205(99)00104-6

Cited by 78 publications

(71 citation statements)

References 33 publications

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“…Primary cultures of human skin fibroblasts from healthy subjects were obtained as described previously (Thellung et al, 1999). Fibroblasts from patients with Niemann-Pick disease were obtained from the "Laboratorio di Diagnosi PrePostnatale Malattie Metaboliche" (Istituto G. Gaslini, Genova Italy) using specimens from the collection "Cell lines and DNA bank from patients affected by genetic diseases".…”

Section: Cell Culturementioning

confidence: 99%

Basic Fibroblast Growth Factor Activates Endothelial Nitric-Oxide Synthase in CHO-K1 Cells via the Activation of Ceramide Synthesis

Florio¹,

Arena

Pattarozzi

et al. 2003

Mol Pharmacol

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In this study, we analyzed the intracellular mechanisms leading to basic fibroblast growth factor (bFGF)-dependent production of NO in Chinese hamster ovary (CHO)-K1 cells and a possible physiological role for such an effect. bFGF induces NO production through the activation of the endothelial form of NO synthase (eNOS), causing a subsequent increase in the cGMP levels. In these cells, the activation of eNOS by bFGF is Ca 2ϩ -and mitogen-activated protein kinase-independent. The translocation of the enzyme from the plasma membrane, where it is located in caveolae bound to caveolin 1, to the cytosol is the crucial step for the synthesis of NO through the eNOS isoform. We demonstrate that bFGF activates a sphingomyelinase to synthesize ceramide, which, in turn, allows the dissociation of eNOS from caveolin 1 and its translocation to the cytosol in the active form, where it catalyzes the synthesis of NO. In fact, drugs interfering with sphingomyelinase activity blocked bFGF activation of eNOS, and an increase in ceramide content was detected after bFGF treatment. Moreover, in fibroblasts derived from patients with Niemann-Pick disease, in which the enzyme is genetically inactive, bFGF is unable to elicit eNOS activation. The NO produced after bFGF treatment, through the activation of guanylyl cyclase and protein kinase G, mediates a mitogenactivated protein kinase-independent cell proliferation. In conclusion, our data show that, in CHO-K1 cells, bFGF regulates the activity of eNOS through a novel intracellular pathway, involving the induction of ceramide synthesis and that the NO released participates in bFGF proliferative activity.Nitric oxide (NO) is an important intracellular and intercellular mediator involved in the modulation of many physiological processes in different tissues, including blood flow regulation, platelet aggregation, smooth muscle relaxation, apoptosis, central and peripheral neurotransmission, and different neuroendocrine responses (Moncada and Higgs, 1993;Nathan and Xie, 1994).NO is synthesized by a family of three distinctive isoforms of nitric-oxide synthase (NOS), named after the tissues in which they were originally described. Neuronal and endothelial NOS [nNOS (or NOS I) and eNOS (or NOS III), respectively] are Ca 2ϩ /calmodulin-dependent enzymes constitutively expressed not only in neuronal and endothelial cells

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Section: Cell Culturementioning

confidence: 99%

Basic Fibroblast Growth Factor Activates Endothelial Nitric-Oxide Synthase in CHO-K1 Cells via the Activation of Ceramide Synthesis

Florio¹,

Arena

Pattarozzi

et al. 2003

Mol Pharmacol

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“…[34][35][36] The favorable effect on cell proliferation appears to be mediated by the activation of purinergic A 2A receptor. 32,37 Therefore, in light of this evidence, we aimed to assess the ability of PDRN to restore blood flow in a rat model of ischemic skin flaps after systemic administration.…”

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confidence: 99%

Polydeoxyribonucleotide restores blood flow in an experimental model of ischemic skin flaps

Polito

Bitto

Galeano

et al. 2012

Journal of Vascular Surgery

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“…PDRN is helpful for normal matrix formation by shortening the healing period through its prompt tissue regeneration and wound healing and by promoting the creation of collageneous/ noncollageneous protein. Also, it is characterized by being effective in tissue regeneration by promoting cell regeneration in cellular stage [9][10][11][12][13][14] . In 2008, an autogenous bone graft material was developed from extracted teeth, and subjected to many basic and clinical studies.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of Autogenous Tooth Bone Graft Combined with Growth Factor: Prospective Cohort Study

Ahn¹,

Kim²,

Yun³

et al. 2013

Journal of Korean Dental Science

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Polydeoxyribonucleotides enhance the proliferation of human skin fibroblasts: Involvement of A2 purinergic receptor subtypes

Cited by 78 publications

References 33 publications

Basic Fibroblast Growth Factor Activates Endothelial Nitric-Oxide Synthase in CHO-K1 Cells via the Activation of Ceramide Synthesis

Basic Fibroblast Growth Factor Activates Endothelial Nitric-Oxide Synthase in CHO-K1 Cells via the Activation of Ceramide Synthesis

Polydeoxyribonucleotide restores blood flow in an experimental model of ischemic skin flaps

Effectiveness of Autogenous Tooth Bone Graft Combined with Growth Factor: Prospective Cohort Study

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