Polydatin protects cardiac function against burn injury by inhibiting sarcoplasmic reticulum Ca2+ leak by reducing oxidative modification of ryanodine receptors

Jiang, Xin; Liu, Wenjuan; Deng, Jianxin; Lan, Liqin; Xue, Xiang; Zhang, Cuicui; Cai, Gaorui; Luo, Xinping; Liu, Jie

doi:10.1016/j.freeradbiomed.2013.02.030

Cited by 45 publications

(44 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The intracellular ROS level was detected as described previously [8,20]. In brief, NRVMs were loaded with 10 μmol/l 5-(and-6)-chloromethyl-dichlorodihydrofluoroscein diacetate (DCFDA; Invitrogen) for 30 min at room temperature, followed by 15 min for de-esterification.…”

Section: Methodsmentioning

confidence: 99%

“…the number of reduced cysteines, in RyR2 in cardiomyocytes was determined with the monobromobimane (mBB; Calbiochem, San Diego, Calif., USA) ﬂuorescence technique [8]. Heavy SR vesicles were prepared from different groups of cells under nonreducing conditions.…”

Section: Methodsmentioning

confidence: 99%

“…In myocardial ischemia-reperfusion, a hyperactive RyR-mediated SR Ca 2+ leak has been suggested to contribute to ventricular arrhythmia, myocardial infarction and left ventricular remodeling [7]. In failing heart, SR Ca 2+ leak impairs cardiac contractile function and induces ventricular arrhythmia [8,9,10]. Abnormal RyR function has also been demonstrated in streptozotocin-induced type 1 diabetes [11], although the pathological significance is not well characterized.…”

Section: Introductionmentioning

confidence: 99%

“…It is well documented that RyR is sensitive to the intracellular redox status due to rich free thiol groups in its structure. An increase in intracellular oxidative stress upregulates RyR activity, resulting in an SR leak [8,13,14,15]. Given that the AGE/RAGE signal induces ROS overproduction, we hypothesized that the AGE/RAGE signal increased SR Ca 2+ leak by increasing RyR activity and consequently impairing cardiac Ca 2+ homeostasis.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Effects of Advanced Glycation End Products on Calcium Handling in Cardiomyocytes

Yan

Luo

et al. 2014

Cardiology

Self Cite

View full text Add to dashboard Cite

Background and Aims: Advanced glycation end products (AGEs) accumulate in diabetes and the engagement of receptor for AGE (RAGE) by AGEs contributes to the pathogenesis of diabetic cardiomyopathy. This study aims to investigate the effects of AGE/RAGE on ryanodine receptor (RyR) activity and Ca²⁺ handling in cardiomyocytes to elucidate the possible mechanism underlying cardiac dysfunction in diabetic cardiomypathy. Methods and Results: Confocal imaging Ca²⁺ spark, the elementary Ca²⁺ release event reflecting RyR activity in intact cell, as well as SR Ca²⁺ content and systolic Ca²⁺ transient were performed in cultured neonatal rat ventricular myocytes. The results show that 50 mg/ml AGE increased the frequency of Ca²⁺ sparks by 160%, while 150 mg/ml AGE increased it by 53%. AGE decreased the amplitude, width and duration of Ca²⁺ sparks. Blocking RAGE with anti-RAGE IgG completely abolished the alteration of Ca²⁺ sparks. The SR Ca²⁺ content indicated by the amplitude (ΔF/F0) of 20 mM caffeine-elicited Ca²⁺ transient was significantly decreased by 150 mg/ml AGE. In parallel, the amplitude of systolic Ca²⁺ transient evoked by 1 Hz-field stimulation was remarkably decreased by 150 mg/ml AGE. The anti-RAGE antibody completely restored the impaired SR load and systolic Ca²⁺ transient. Conclusion: AGE/RAGE signal enhanced Ca²⁺ spark-mediated SR Ca²⁺ leak, causing partial depletion of SR Ca²⁺ content and consequently decreasing systolic Ca²⁺ transient, which may contribute to contractile dysfunction in diabetic cardiomyopathy.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of Advanced Glycation End Products on Calcium Handling in Cardiomyocytes

Yan

Luo

et al. 2014

Cardiology

Self Cite

View full text Add to dashboard Cite

show abstract

“…Polydatin (PD), extracted from the roots of Polygonum cuspidatum Sieb, is widely applied in traditional Chinese remedies [16] . Some studies have indicated that PD can improve heart function and ameliorate renal injury through antioxidative and anti-inflammatory effects [17,18] . However, there are no reports concerning the effects of PD on mastitis, and the mechanism involved is not clear.…”

Section: Original Articlementioning

confidence: 99%

Polydatin ameliorates Staphylococcus aureus-induced mastitis in mice via inhibiting TLR2-mediated activation of the p38 MAPK/NF-κB pathway

et al. 2016

View full text Add to dashboard Cite

Recent studies show that Polydatin (PD) extracted from the roots of Polygonum cuspidatum Sieb, a widely used traditional Chinese remedies, possesses anti-inflammatory activity in several experimental models. In this study, we investigated the anti-inflammatory effects of PD on Staphylococcus aureus-induced mastitis in mice and elucidated the potential mechanisms. In mice with S aureusinduced mastitis, administration of PD (15, 30, 45 mg/kg, ip) or dexamethasone (Dex, 5 mg/kg, ip) significantly suppressed the infiltration of inflammatory cells, ameliorated the mammary structural damage, and inhibited the activity of myeloperoxidase, a biomarker of neutrophils accumulation. Furthermore, PD treatment dose-dependently decreased the levels of TNF-α, IL-1β, IL-6 and IL-8 in the mammary gland tissues. PD treatment also dose-dependently decreased the expression of TLR2, MyD88, IRAK1, IRAK4 and TRAF6 as well as the phosphorylation of TAK1, MKK3/6, p38 MAPK, IκB-α and NF-κB in the mammary gland tissues. In mouse mammary epithelial cells (mMECs) infected by S aureus in vitro, pretreatment with PD dose-dependently suppressed the upregulated pro-inflammatory cytokines and signaling proteins, and the nuclear translocation of NF-κB p65 and AP-1. A TLR2-neutralizing antibody mimicked PD in its suppression on S aureus-induced upregulation of MyD88, p-p38 and p-p65 levels in mMECs. PD (50, 100 μg/mL) affected neither the growth of S aureus in vitro, nor the viability of mMECs. In conclusion, PD does not exhibit antibacterial activity against S aureus, its therapeutic effects in mouse S aureus-induced mastitis depend on its ability to down-regulate pro-inflammatory cytokine levels via inhibiting TLR2-mediated activation of the p38 MAPK/NF-κB signaling pathway.

show abstract

Fermentation ofSmilax chinaroot byAspergillus usamiandSaccharomyces cerevisiaepromoted concentration of resveratrol and oxyresveratrol and the free-radical scavenging activity

Yoon

Yang

Suh

et al. 2014

J. Sci. Food Agric.

View full text Add to dashboard Cite

show abstract

Polydatin protects cardiac function against burn injury by inhibiting sarcoplasmic reticulum Ca2+ leak by reducing oxidative modification of ryanodine receptors

Cited by 45 publications

References 29 publications

Effects of Advanced Glycation End Products on Calcium Handling in Cardiomyocytes

Effects of Advanced Glycation End Products on Calcium Handling in Cardiomyocytes

Polydatin ameliorates Staphylococcus aureus-induced mastitis in mice via inhibiting TLR2-mediated activation of the p38 MAPK/NF-κB pathway

Fermentation ofSmilax chinaroot byAspergillus usamiandSaccharomyces cerevisiaepromoted concentration of resveratrol and oxyresveratrol and the free-radical scavenging activity

Contact Info

Product

Resources

About