Polydatin promotes the osteogenic differentiation of human bone mesenchymal stem cells by activating the BMP2-Wnt/β-catenin signaling pathway

Chen, Xiaojun; Shen, Ying-Shan; He, Mincong; Yang, Fan; Yang, Peng; Pang, Fengxiang; He, Wei; Cao, Yanming; Wei, Qiushi

doi:10.1016/j.biopha.2019.108746

Cited by 115 publications

(105 citation statements)

References 48 publications

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“…We therefore investigated the exact antiosteoporotic mechanism of a prominent Chinese compound, PD, in vitro and in vivo. As described by our results, PD increased the proliferation and osteogenic differentiation of hBMSCs without cytotoxicity, as proven by the significant increases in calcium nodules and expression of osteoblast markers, which is consistent with the results of our previous study [31]. In our previous study, we also found that the PD-induced expression of BMP2 was not blocked by a specific Wnt signal inhibitor.…”

Section: Discussionsupporting

confidence: 93%

“…In our previous study, we also found that the PD-induced expression of BMP2 was not blocked by a specific Wnt signal inhibitor. Furthermore, it is illustrated that PD activated the BMP2-induced Wnt signaling pathway through enhancing the accumulation and nuclear translocation of β-catenin and subsequently promoted the osteogenesis of hBMSCs [31]. Further experiments found that knockdown of TAZ hampered the effect of PD on osteogenesis through the BMP2-Wnt/β-catenin signaling pathway.…”

Section: Discussionmentioning

confidence: 94%

“…PD was recently found to alleviate osteoporosis symptoms in the ovariectomized (OVX) mouse model by upregulating the expression of β-catenin [30]. Previously, we showed the osteogenetic effect of PD on hBMSCs through the BMP-Wnt/β-catenin pathway [31].…”

Section: Introductionmentioning

confidence: 99%

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RETRACTED ARTICLE: Polydatin improves osteogenic differentiation of human bone mesenchymal stem cells by stimulating TAZ expression via BMP2-Wnt/β-catenin signaling pathway

et al. 2020

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Objectives: Polydatin (PD), extracted from Polygonum cuspidatum, has shown potential therapeutic applications due to its antiosteoporotic and anti-inflammatory activities. Our previous study suggested that PD promotes the osteogenesis of human bone marrow stromal cells (hBMSCs) via the BMP2-Wnt/β-catenin pathway. The aim of our present study was to further explore the role of PD-mediated regulation of Tafazzin (TAZ), a transcriptional coactivator with a PDZ-binding motif, in osteogenesis. Materials and methods: hBMSCs were isolated and treated with PD at various concentrations. Alizarin red staining and RT-qPCR were performed to identify calcium complex deposition in hBMSCs as well as the expression of specific osteoblast-related markers, respectively, in each group. Next, TAZ-silenced hBMSCs were generated by lentivirus-produced TAZ shRNA. After treatment with PD, the osteogenic abilities of the TAZ-silenced and control hBMSCs were estimated by ALP activity assay, and expression of the TAZ protein was detected by Western blot analysis and immunofluorescence staining. In vitro, an ovariectomized (OVX) mouse model was established and used to evaluate the effect of PD on bone destruction by micro-CT, immunohistochemistry, and ELISA.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 94%

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RETRACTED ARTICLE: Polydatin improves osteogenic differentiation of human bone mesenchymal stem cells by stimulating TAZ expression via BMP2-Wnt/β-catenin signaling pathway

et al. 2020

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“…Ligustilide, an ingredient from Angelica sinensis , had the function in promoting osteoblast differentiation of preosteogenic cell line MC3T3-E1 and BMSCs and inducing the phosphorylation and activity of EGFR and ERK1/2, through the fast response pathway mediated by the estrogen membrane receptor GPR30 [ 45 ]. Accumulating results showed that a large number of Chinese herbal ingredients significantly promoted BMSC differentiation, such as salvianolic acid B at 5 μ M, tanshinone IIA at 1 and 5 μ M, polydatin at 30 μ M, salidroside at 0.5–10 μ M, puerarin at 10 μ M, ginkgolide B at μ M, THSG at 6.25–25 μ g/ml, catalpol at 50 μ M, and baicalin at 50 μ M [ 46 – 54 ], among which salvianolic acid B and polydatin can increase the ALP activity and upregulate the expression of osteogenic genes COLI, OPN, OCN, Runx2, osterix, and DLX5 in human BMSCs [ 46 , 47 ]. The detailed signaling pathways of active ingredients inducing osteoblast differentiation are shown in Figure 4 .…”

Section: Effect Of Chinese Medical Herbs On Stem Cell Differentiatmentioning

confidence: 99%

“…For example, icariin activated the Wnt/ β -catenin signaling pathway during the differentiation of osteoblasts [ 41 , 55 ], and the same pathway was provoked by ginkgolide B and salidroside during neuronal differentiation [ 15 , 56 ]. In addition, in the process of promoting osteoblast differentiation, tanshinone IIA, polydatin, and catalpol also selectively activated the bone morphogenetic proteins (BMP)/Wnt signaling pathway [ 47 , 48 , 53 ]. Surprisingly, salvianolic acid B promoted bone marrow-derived mesenchymal stem cells to differentiate into alveolar endothelial cell type I and hepatocytes in the same Wnt pathway [ 57 , 58 ].…”

Section: Signaling Pathways In Stem Cell Differentiation Activatedmentioning

confidence: 99%

Effect of Active Ingredients of Chinese Herbal Medicine on the Rejuvenation of Healthy Aging: Focus on Stem Cells

Wang

Ling

2020

Evidence-Based Complementary and Alternative Medicine

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Stem cells (SCs) are special types of cells with the ability of self-renewal and multidirectional differentiation. As the organism ages, the ability to maintain homeostasis and regeneration deteriorates and the number and activity of stem cells decline. Theoretically, the restoration of stem cells might reverse aging. However, due to their own aging, donor-derived immune rejection, and difficulties in stem cell differentiation control, a series of problems need to be solved to realize the potential for clinical application of stem cells. Chinese herbal medicine is a nature drug library which is suitable for the long-term treatment of aging-related diseases. Modern pharmacological studies have revealed that many active ingredients of Chinese herbal medicines with the effect of promoting stem cells growth and differentiation mainly belong to “reinforcing herbs.” In recent years, exploration of natural active ingredients from Chinese herbal medicines for delaying aging, improving the stem cell microenvironment, and promoting the proliferation and differentiation of endogenous stem cells has attracted substantial attention. This article will focus on active ingredients from Chinese herbs-mediated differentiation of stem cells into particular cell type, like neural cells, endothelial cells, cardiomyocytes, and osteoblasts. We will also discuss the effects of these small molecules on Wnt, Sonic Hedgehog, Notch, eNOS-cGMP, and MAP kinase signal transduction pathways, as well as reveal the role of estrogen receptor α and PPAR γ on selectively promoting or inhibiting stem cells differentiation. This review will provide new insights into the health aging strategies of active ingredients in Chinese herbal medicine in regenerative medicine.

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Delivery of MiR335‐5p‐Pendant Tetrahedron DNA Nanostructures Using an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid‐Associated Osteonecrosis

Yang

Luo

et al. 2021

Adv Healthcare Materials

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Corticosteroids-induced Dickkopf-1 (DKK1) upregulation and Wnt signaling inhibition result in bone metabolism disorder and steroid-associated osteonecrosis (SAON). Implanting biomaterials to regulate the Wnt pathway is a promising method to repair challenging bone defects associated with SAON. Here, tetrahedral DNA nanostructures (TDNs) are fabricated as gene carriers to deliver MiR335-5p, which targets DKK1 translation. Heparin lithium hydrogel (Li-hep-gel) is synthesized to act as a lithium and MiR@TDNs delivery agent. Finally, the repair effects on challenging bone defect in SAON using a MiR@TDNs/Li-hep-gel composite are assessed in vivo. The results reveal that MiR@TDNs are absorbed by bone mesenchymal stem cells (BMSCs) and increase cell viability and reduce apoptosis. Moreover, MiR@TDNs promote alkaline phosphatase expression and calcium nodular deposition, decrease lipid droplet expression of BMSCs, and improve vascular endothelial growth factor secretion and vascular-like structure formation in vitro. After MiR@TDNs/Li-hep-gel is implanted into the SAON model, the internal bone defect of osteonecrosis is repaired with a large area of new bone accompanied with neovascularization and reduced empty lacunae. In conclusion, MiR@TDNs/Li-hep-gel can provide dual delivery of lithium and MiR@TDNs, which synergistically upregulate the Wnt signaling pathway, enhancing bone regeneration in challenging bone defects, and can be potentially used in SAON repair.

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Polydatin promotes the osteogenic differentiation of human bone mesenchymal stem cells by activating the BMP2-Wnt/β-catenin signaling pathway

Cited by 115 publications

References 48 publications

RETRACTED ARTICLE: Polydatin improves osteogenic differentiation of human bone mesenchymal stem cells by stimulating TAZ expression via BMP2-Wnt/β-catenin signaling pathway

RETRACTED ARTICLE: Polydatin improves osteogenic differentiation of human bone mesenchymal stem cells by stimulating TAZ expression via BMP2-Wnt/β-catenin signaling pathway

Effect of Active Ingredients of Chinese Herbal Medicine on the Rejuvenation of Healthy Aging: Focus on Stem Cells

Delivery of MiR335‐5p‐Pendant Tetrahedron DNA Nanostructures Using an Injectable Heparin Lithium Hydrogel for Challenging Bone Defects in Steroid‐Associated Osteonecrosis

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