Polydatin down-regulates the phosphorylation level of Creb and induces apoptosis in human breast cancer cell

Abstract

Polydatin (PD), a component isolated from Polygonum cuspidatum, has a number of biological functions. However, the antitumor activity of PD has been poorly investigated. In this study, the effect of PD on cell proliferation was evaluated by thiazolyl blue tetrazolium bromide assay. Cell cycle distribution and apoptosis were investigated by flow cytometry. The phosphorylation levels of panel of phosphor-kinases were detected by human phospho-kinase arrays. The expression of several proteins associated with cell cycle and apoptosis were analyzed by Western blot analysis. Results showed that PD effectively inhibited the growth of MDA-MB-231 and MCF-7 breast cancer cell lines. Cell cycle analysis demonstrated that PD induced S-phase cell cycle arrest. Human phosphor-kinase arrays showed that the phosphorylation level of cAMP response element-bingding proteins(Creb) was down-regulated, and the results were further confirmed by Western blot analysis. Western blot analysis showed that the expression of protein of cyclin D1 decreased in a time- and dose- dependent manner. Results suggest that PD is a potential therapeutic natural compound.

Targeting the ROS/PI3K/AKT/HIF‐1α/HK2 axis of breast cancer cells: Combined administration of Polydatin and 2‐Deoxy‐d‐glucose

“…PD is known to inhibit proliferation and induce apoptosis in breast cancer and colorectal cancer. 26,27 Therefore, we investigated disrupt the extracellular matrix (ECM) and then induce cancer cell migration and invasion. 28,29 In vitro, increased autocrine vascular endothelial growth factor (VEGF) is also considered to be one of the hallmarks of cancer invasion.…”

The EO771 mammary cancer cell line displays a luminal B phenotype and it is sensitive to anti-estrogen treatments

“…Chen et al have shown that downregulation of cAMP-response element binding protein (CREB) was associated with inhibition of mammary tumor cell growth by a mechanism that appears to be independent of ER as acting on both triple negative cells (MDA-MB-231) and ER+ cells (MCF-7) 21 . Knowing that this pathway is found activated during resistance to tamoxifen 22 , the effects of this drug on CREB pathway were investigated in EO771 cells.…”

Uncovering the action mechanism of polydatinvianetwork pharmacological target prediction

“…Notably, all of these pathways were associated with cancer-related diseases, in line with the notion that PD showed the potential as anti-tumor agent. 11,12 Based on target predicting and pathway enrichment analysis, a systematic network was constructed using Cytoscape 3.2.1. As shown in Fig.…”

“…[4][5][6][7][8][9][10] Furthermore, recent studies have shown that PD may act as an anti-tumor agent by inhibiting cell proliferation and inducing apoptosis in a variety of tumors. 11,12 However, current knowledge about the medicinal characteristics as well as the action mechanism of PD is still fragmented, and its system-level mechanism warrants further investigation.…”