Polydatin ameliorates renal ischemia/reperfusion injury by decreasing apoptosis and oxidative stress through activating sonic hedgehog signaling pathway

Meng, Qiu-Hong; Liu, Hongbao; Wang, Jianbo

doi:10.1016/j.fct.2016.07.032

Cited by 55 publications

(35 citation statements)

References 44 publications

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“…In agreement with these observations, pretreatment with PD prevented the activation of caspase-3 and the increase in Bax/Bcl-2 ratio, thus confirming the cytoprotective properties of PD against MGO-induced apoptosis. These results were consistent with previous studies which demonstrated that PD prevented cell apoptosis against burn [ 34 ], shock [ 35 ], and ischemia/reperfusion injury [ 24 , 36 ].…”

Section: Discussionsupporting

confidence: 93%

“…It is also detected in red wine, grape hop cones, peanuts, cocoa-containing products, and many daily diets [ 19 ]. Previous studies have indicated that PD has a number of biological activities, such as protective effects against shock [ 20 – 22 ] and ischemia/reperfusion damage [ 23 , 24 ], inhibiting platelet aggregation [ 25 ], reducing lipid peroxidation [ 26 ], and so on. It has also been demonstrated that PD acts as an antioxidant agent [ 24 , 27 ] or a mitochondria protector [ 21 , 22 ] to prevent severe disease.…”

Section: Introductionmentioning

confidence: 99%

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Polydatin Prevents Methylglyoxal-Induced Apoptosis through Reducing Oxidative Stress and Improving Mitochondrial Function in Human Umbilical Vein Endothelial Cells

Pang

Chen

Deng

et al. 2017

Oxidative Medicine and Cellular Longevity

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Methylglyoxal (MGO), an active metabolite of glucose, has been reported to induce vascular cell apoptosis in diabetic complication. Polydatin (PD), a small natural compound from Polygonum cuspidatum, has a number of biological functions, such as antioxidative, anti-inflammatory, and nephroprotective properties. However, the protective effects of PD on MGO-induced apoptosis in endothelial cells remain to be elucidated. In this study, human umbilical vein endothelial cells (HUVECs) were used to explore the effects of PD on MGO-induced cell apoptosis and the possible mechanism involved. HUVECs were pretreated with PD for 2 h, followed by stimulation with MGO. Then cell apoptosis, reactive oxygen species (ROS) generation, mitochondrial membrane potential (MMP) impairment, mitochondrial morphology alterations, and Akt phosphorylation were assessed. The results demonstrated that PD significantly prevented MGO-induced HUVEC apoptosis. PD pretreatment also significantly inhibited MGO-induced ROS production, MMP impairment, mitochondrial morphology changes, and Akt dephosphorylation. These results and the experiments involving N-acetyl cysteine (antioxidant), Cyclosporin A (mitochondrial protector), and LY294002 (Akt inhibitor) suggest that PD prevents MGO-induced HUVEC apoptosis, at least in part, through inhibiting oxidative stress, maintaining mitochondrial function, and activating Akt pathway. All of these data indicate the potential application of PD for the treatment of diabetic vascular complication.

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Section: Discussionsupporting

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Polydatin Prevents Methylglyoxal-Induced Apoptosis through Reducing Oxidative Stress and Improving Mitochondrial Function in Human Umbilical Vein Endothelial Cells

Pang

Chen

Deng

et al. 2017

Oxidative Medicine and Cellular Longevity

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“…Resveratrol protects against ischemic injury after stroke via SHH signaling (Yu et al, ) and it can induce bone mesenchymal stem cells to differentiate into neuronal‐like cells by activating a SHH signaling pathway in primary cilia (Huang et al, ). In addition, several studies verified that polydatin, a glucoside of resveratrol, definitively impacted the activation of SHH signaling pathway to exert various biological activities in different models (Ji et al, ; Meng, Liu, & Wang, ; Meng, Liu, & Wang, ).…”

Section: Discussionmentioning

confidence: 96%

Sonic hedgehog signaling mediates resveratrol to improve maturation of pig oocytes in vitro and subsequent preimplantation embryo development

Lee

Jin

Taweechaipaisankul

et al. 2018

Journal Cellular Physiology

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The beneficial effects of resveratrol on in vitro maturation (IVM) have been explained mainly by indirect antioxidant effects and limited information is available on the underlying mechanism by which resveratrol acts directly on porcine cumulus oocyte complexes (COCs). Recently, several studies reported that sonic hedgehog (SHH) signaling mediates resveratrol to exert its biological activities. Furthermore, SHH is an important signaling molecule for follicle development, oocyte maturation, and embryo development. Therefore, to elucidate the relationship between resveratrol and SHH signaling, we designed three groups: (i) control; (ii) resveratrol; and (iii) resveratrol with cyclopamine (SHH signaling inhibitor). We evaluated the effects of these agents on cumulus expansion, oocyte maturation, embryo development after parthenogenetic activation, expression levels of mRNAs in cumulus cells, oocytes and blastocysts, and protein expression in COCs. Resveratrol significantly increased the proportion of COCs exhibiting complete cumulus expansion (degree 4), oocyte nuclear maturation, cleavage and blastocyst formation rates and total cell numbers, which were blocked in the presence of cyclopamine. At the same time, a significant increase in the expression levels of mRNAs related to cumulus expansion, oocyte maturation and SHH signaling-related mRNAs and proteins from the resveratrol treatment group was also inhibited by simultaneous addition of cyclopamine. In conclusion, our results indicate that SHH signaling mediates resveratrol to improve porcine cumulus expansion, oocyte maturation, and subsequent embryo development.

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“…Studies have shown that LTA can induce a high level of intracellular ROS in various cell types, leading to injury, such as inflammation . However, resveratrol ameliorates inflammation in skeletal muscle cells by attenuating oxidative stress , and PD has been shown to ameliorate renal ischaemia/reperfusion injury by decreasing apoptosis and oxidative stress . However, it is not known whether PD plays a role in endometritis and its underlying mechanism.…”

Section: Introductionmentioning

confidence: 99%

Polydatin reduces Staphylococcus aureus lipoteichoic acid‐induced injury by attenuating reactive oxygen species generation and TLR2‐NFκB signalling

Zhao

Jiang

et al. 2017

J Cellular Molecular Medi

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Staphylococcus aureus (S. aureus) causes severe inflammation in various infectious diseases, leading to high mortality. The clinical application of antibiotics has gained a significant curative effect. However, it has led to the emergence of various resistant bacteria. Therefore, in this study, we investigated the protective effect of polydatin (PD), a traditional Chinese medicine extract, on S. aureus lipoteichoic acid (LTA)‐induced injury in vitro and in vivo. First, a significant improvement in the pathological conditions of PD in vivo was observed, suggesting that PD had a certain protective effect on LTA‐induced injury in a mouse model. To further explore the underlying mechanisms of this protective effect of PD, LTA‐induced murine macrophages were used in this study. The results have shown that PD could reduce the NF‐κB p65, and IκBα phosphorylation levels increased by LTA, resulting in a decrease in the transcription of pro‐inflammatory factors, such as TNF‐α, IL‐1β and IL‐6. However, LTA can not only activate NF‐κB through the recognition of TLR2 but also increase the level of intracellular reactive oxygen species (ROS), thereby activating NF‐κB signalling. We also detected high levels of ROS that activate caspases 9 and 3 to induce apoptosis. In addition, using a specific NF‐κB inhibitor that could attenuate apoptosis, namely NF‐κB p65, acted as a pro‐apoptotic transcription factor in LTA‐induced murine macrophages. However, PD could inhibit the generation of ROS and NF‐κB p65 activation, suggesting that PD suppressed LTA‐induced injury by attenuating ROS generation and TLR2‐NFκB signalling.

show abstract

Polydatin ameliorates renal ischemia/reperfusion injury by decreasing apoptosis and oxidative stress through activating sonic hedgehog signaling pathway

Cited by 55 publications

References 44 publications

Polydatin Prevents Methylglyoxal-Induced Apoptosis through Reducing Oxidative Stress and Improving Mitochondrial Function in Human Umbilical Vein Endothelial Cells

Polydatin Prevents Methylglyoxal-Induced Apoptosis through Reducing Oxidative Stress and Improving Mitochondrial Function in Human Umbilical Vein Endothelial Cells

Sonic hedgehog signaling mediates resveratrol to improve maturation of pig oocytes in vitro and subsequent preimplantation embryo development

Polydatin reduces Staphylococcus aureus lipoteichoic acid‐induced injury by attenuating reactive oxygen species generation and TLR2‐NFκB signalling

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