Polycystic ovary syndrome (PCOS) and kisspeptin – A Sri Lankan study

Umayal, B; Jayakody, Surangi; Chandrasekharan, N.V.; Wijesundera, W.S.S.; Wijeyaratne, Chandrika

doi:10.4103/jpgm.jpgm_683_17

Cited by 25 publications

(6 citation statements)

References 31 publications

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“…In addition, the positive likelihood ratio (LH) was 6.55 which indicates that this test can increase the index of suspicion for confirming the PCOS diagnosis by 35–40%, while its negative LH (0.29) indicates it has 25–30% ability to exclude the diagnosis of PCOS; this was in agreement with a recent Turkish study in 2018 with kisspeptin levels in a normal ovarian reserve which were 4.65 ± 2.16 ng/mL, and 5.76 ± 2.11 in those with a high ovarian reserve [ 12 ]. Jeon et al showed similar findings (10.65 ± 6.14 vs. 6.51 ± 3.13 pmol/l, p < 0.001) [ 16 ] and were in agreement with Umayal et al (4.713 vs. 4.127 nmol/L, p value = 0.033) [ 17 ]. Other studies such as Chen et al and Joen et al both showed increased kisspeptin level in women with PCOS [ 16 , 18 ].…”

Section: Discussionsupporting

confidence: 83%

Metastatin as a Marker for Hyperandrogenemia in Iraqi Women with Polycystic Ovary Syndrome

Abdalqader

Hussein

2020

Obstetrics and Gynecology International

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Objective. Kisspeptin 1 might reflect increased androgen level in polycystic ovarian syndrome instead of other markers. Study Design. A case control study was performed in Al-Yarmouk Teaching Hospital from 1st of July 2016 to 1st of July 2017; it involved 87 women divided into two groups: 44 women diagnosed as PCOS, 22 women with BMI ≥ 25 kg/m2 and 22 women with BMI < 25 kg/m2, and another 43 women without PCOS, 22 women with BMI ≥ 25 kg/m2 and 21 women with BMI < 25 kg/m2. Hormonal, metabolic profiles, and hirsutism scores, as well as serum kisspeptin level, were assessed by using Human Kisspeptin 1(KISS-1) ELISA Kit. The blood samples between days 2 and 5 of menstrual cycle were drawn by an disposable sterile syringe and collected in EDTA containing tubes (as anticoagulant), and the hormonal profile was measured using a biotech ELISA reader. Result. Serum level of kisspeptin was significantly higher in PCOS compared to control (322.4 vs. 235.3 ng/L, respectively). There was no significant difference in age, BMI, and parity between control and PCOS; the frequency of hirsutism, acne, elevated LH, and increased free testosterone (fTT) were significantly higher in PCOS compared to control. Kisspeptin shows a direct significant correlation with hirsutism and fTT (r = 0.648, 0.238, respectively). In ROC analysis, kisspeptin had AUC (95% CI) = 0.874 (0.785–0.935) for predicting PCOS. Conclusion. Kisspeptin levels might be used as a marker for hyperandrogenemia in polycystic ovarian syndrome.

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Section: Discussionsupporting

confidence: 83%

Metastatin as a Marker for Hyperandrogenemia in Iraqi Women with Polycystic Ovary Syndrome

Abdalqader

Hussein

2020

Obstetrics and Gynecology International

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“…Sample size was calculated for a case-control design using Schlesselman method [ 14 ], as in our previous reports on other genetic aspects of the same study sample [ 15 , 16 ].…”

Section: Methodsmentioning

confidence: 99%

Association of Kiss1 and GPR54 Gene Polymorphisms with Polycystic Ovary Syndrome among Sri Lankan Women

Branavan

Muneeswaran

Wijesundera

et al. 2019

BioMed Research International

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Polycystic ovary syndrome (PCOS) is the commonest endocrine disorder affecting women of reproductive age. Its aetiology, though yet unclear, is presumed to have an oligogenic basis interacting with environmental factors. Kisspeptins are peptide products of Kiss1 gene that control the hypothalamic pituitary (HPG) axis by acting via G protein-coupled receptor known as GPR54. There is paucity of data on the role of Kiss1 and GPR54 gene in PCOS. We aimed to identify the polymorphisms in Kiss1 and GPR54 genes and explore their association with serum kisspeptin levels among Sri Lankan women with well-characterized PCOS. Consecutive women with PCOS manifesting from adolescence (n=55) and adult controls (n=110) were recruited. Serum kisspeptin and testosterone levels were determined by ELISA method. Whole gene sequencing was performed to identify the polymorphisms in Kiss1 and GPR54 genes. Serum kisspeptin and testosterone concentrations were significantly higher in women with PCOS than controls: kisspeptin 4.873nmol/L versus 4.127nmol/L; testosterone 4.713nmol/L versus 3.415 nmol/L, p<0.05. Sequencing the GPR54 gene revealed 5 single nucleotide polymorphisms (SNPs), rs10407968, rs1250729403, rs350131, chr19:918686, and chr19:918735, with two novel SNPs (chr19:918686 and chr19:918735), while sequencing the Kiss1 gene revealed 2 SNPs, rs5780218 and rs4889. All identified SNPs showed no significant difference in frequency between patients and controls. GPR54 gene rs350131 polymorphism (G/T) was detected more frequently in our study population. The heterozygous allele (AG) of GPR54 gene novel polymorphism chr19:918686 showed a marginal association with serum kisspeptin levels (p=0.053). Genetic variations in GPR54 and Kiss1 genes are unlikely to be associated with PCOS among Sri Lankan women manifesting from adolescence. Meanwhile the heterozygous allele of chr19:918686 is probably associated with serum kisspeptin concentrations, which suggests a potential role in the aetiology of PCOS.

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“…These findings imply that the KNDy system may be an attractive therapeutic target to modulate AR-driven neuroendocrine activity in PCOS patients. Indeed, increased kisspeptin has been reported in several populations of PCOS patients (Albalawi et al 2018, Katulski et al 2018, Umayal et al 2019. Of note, not all populations of women with PCOS exhibit changes in kisspeptin levels (Daghestani 2018, Katulski et al 2018, inferring alterations in the KNDy system may be related to differences in PCOS phenotype or severity.…”

Section: Current Implications and Future Directions From Basic Researmentioning

confidence: 99%

Androgens and ovarian function: translation from basic discovery research to clinical impact

Walters

Paris

Aflatounian

et al. 2019

Journal of Endocrinology

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In the last decade, it has been revealed that androgens play a direct and important role in regulating female reproductive function. Androgens mediate their actions via the androgen receptor (AR), and global and cell-specific Ar-knockout mouse models have confirmed that AR-mediated androgen actions play a role in regulating female fertility and follicle health, development and ovulation. This knowledge, along with the clinical data reporting a beneficial effect of androgens or androgen-modulating agents in augmenting in vitro fertilization (IVF) stimulation in women termed poor responders, has supported the adoption of this concept in many IVF clinics worldwide. On the other hand, substantial evidence from human and animal studies now supports the hypothesis that androgens in excess, acting via the AR, play a key role in the origins of polycystic ovary syndrome (PCOS). The identification of the target sites of these AR actions and the molecular mechanisms involved in underpinning the development of PCOS is essential to provide the knowledge required for the future development of novel, mechanism-based therapies for the treatment of PCOS. This review will summarize the basic scientific discoveries that have enhanced our knowledge of the roles of androgens in female reproductive function, discuss the impact these findings have had in the clinic and how a greater understanding of the role androgens play in female physiology may shape the future development of effective strategies to improve IVF outcomes in poor responders and the amelioration of symptoms in patients with PCOS.

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Polycystic ovary syndrome (PCOS) and kisspeptin – A Sri Lankan study

Abstract: Serum kisspeptin levels are higher in Sri Lankan women with PCOS manifesting from adolescence compared with controls regardless of body mass index. We propose serum kisspeptin concentration as a useful marker to recognize PCOS that manifests from adolescence.

Cited by 25 publications

References 31 publications

Metastatin as a Marker for Hyperandrogenemia in Iraqi Women with Polycystic Ovary Syndrome

Metastatin as a Marker for Hyperandrogenemia in Iraqi Women with Polycystic Ovary Syndrome

Association of Kiss1 and GPR54 Gene Polymorphisms with Polycystic Ovary Syndrome among Sri Lankan Women

Androgens and ovarian function: translation from basic discovery research to clinical impact

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