Polycyclic Aromatic Hydrocarbons (PAHs) Mediate Transcriptional Activation of the ATP Binding Cassette Transporter ABCB6 Gene via the Aryl Hydrocarbon Receptor (AhR)

Chavan, Hemantkumar; Krishnamurthy, Partha

doi:10.1074/jbc.m112.371476

Cited by 24 publications

(16 citation statements)

References 57 publications

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“…Cellular Protoporphyrin IX Measurements-Intracellular protoporphyrin IX concentration was measured as previously described (21). Briefly, cells were harvested and washed once with PBS.…”

Section: Materials-8-azido-[␣-mentioning

confidence: 99%

Efficient Purification and Reconstitution of ATP Binding Cassette Transporter B6 (ABCB6) for Functional and Structural Studies

Chavan

Khan

Tegos

et al. 2013

Journal of Biological Chemistry

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Background:The ABCB6 protein is proposed to transport coproporphyrinogen from the cytoplasm into the mitochondria. Results: Purified ABCB6 reconstituted into liposomes demonstrates coproporphyrinogen-stimulated ATP hydrolysis and coproporphyrinogen transport. Conclusion: ABCB6 does not require additional components for substrate-stimulated ATPase activity and substrate transport. Significance: Development of an in vitro system with pure and active ABCB6 for structure and functional studies is indicated.

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“…Cellular Protoporphyrin IX Measurements-Intracellular protoporphyrin IX concentration was measured as previously described (21). Briefly, cells were harvested and washed once with PBS.…”

Section: Materials-8-azido-[␣-mentioning

confidence: 99%

Efficient Purification and Reconstitution of ATP Binding Cassette Transporter B6 (ABCB6) for Functional and Structural Studies

Chavan

Khan

Tegos

et al. 2013

Journal of Biological Chemistry

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“…In summary, PEPT1 overexpression or ABCG2 suppressing caused increase of intracellular PpIX accumulation24. Other studies showed that ABCB6 overexpression induced increase of PpIX12 and ABCB6 suppressing induced decrease of PpIX25 respectively. Therefore, we focused on these 3 transporters.…”

Section: Resultsmentioning

confidence: 68%

Dormant cancer cells accumulate high protoporphyrin IX levels and are sensitive to 5-aminolevulinic acid-based photodynamic therapy

Nakayama

Otsuka

Kobayashi

et al. 2016

Sci Rep

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Photodynamic therapy (PDT) and diagnosis (PDD) using 5-aminolevulinic acid (ALA) to drive the production of an intracellular photosensitizer, protoporphyrin IX (PpIX), are in common clinical use. However, the tendency to accumulate PpIX is not well understood. Patients with cancer can develop recurrent metastatic disease with latency periods. This pause can be explained by cancer dormancy. Here we created uniformly sized PC-3 prostate cancer spheroids using a 3D culture plate (EZSPHERE). We demonstrated that cancer cells exhibited dormancy in a cell density-dependent manner not only in spheroids but also in 2D culture. Dormant cancer cells accumulated high PpIX levels and were sensitive to ALA-PDT. In dormant cancer cells, transporter expressions of PEPT1, ALA importer, and ABCB6, an intermediate porphyrin transporter, were upregulated and that of ABCG2, a PpIX exporter, was downregulated. PpIX accumulation and ALA-PDT cytotoxicity were enhanced by G0/G1-phase arrestors in non-dormant cancer cells. Our results demonstrate that ALA-PDT would be an effective approach for dormant cancer cells and can be enhanced by combining with a cell-growth inhibitor.

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“…P450 Promoter-LUC Reporter Assays in HepG2 Cells-Promoter-reporter luciferase activity assays were performed as previously described (29). Briefly, HepG2 cells at ϳ60% confluency were transfected with either Preparation of Mouse Liver Extracts-Metabolite extraction from liver samples was done as described (36).…”

Section: Methodsmentioning

confidence: 99%

“…Immunoblotting-Western blot analysis of mitochondrial and microsomal proteins was carried out as previously described (28,29). Polyclonal primary antibodies were used to detect P450 oxidoreductase (catalog no.…”

Section: Methodsmentioning

confidence: 99%

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Functional Coupling of ATP-binding Cassette Transporter Abcb6 to Cytochrome P450 Expression and Activity in Liver

Chavan¹,

Li²,

Tessman³

et al. 2015

Journal of Biological Chemistry

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Background: Physiological signals that negatively regulate CYP450s are not well understood. Results: Loss of Abcb6 in mice results in suppression of CYP450 activity. Conclusion: Suppression of P450 activity in Abcb6 deficiency may result from altered endogenous metabolites involved in maintaining homeostasis. Significance: Understanding metabolite alterations in Abcb6 deficiency should help understand the physiological signals and the mechanisms involved in negative regulation of P450s.

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Polycyclic Aromatic Hydrocarbons (PAHs) Mediate Transcriptional Activation of the ATP Binding Cassette Transporter ABCB6 Gene via the Aryl Hydrocarbon Receptor (AhR)

Cited by 24 publications

References 57 publications

Efficient Purification and Reconstitution of ATP Binding Cassette Transporter B6 (ABCB6) for Functional and Structural Studies

Efficient Purification and Reconstitution of ATP Binding Cassette Transporter B6 (ABCB6) for Functional and Structural Studies

Dormant cancer cells accumulate high protoporphyrin IX levels and are sensitive to 5-aminolevulinic acid-based photodynamic therapy

Functional Coupling of ATP-binding Cassette Transporter Abcb6 to Cytochrome P450 Expression and Activity in Liver

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