This study aimed to assess the health status of Carassius gibelio, a comparatively tolerant fish species, in the largest coastal lagoon in Europe – the Curonian Lagoon. Haematocrit and glucose levels were evaluated as reliable biomarkers of stress responses in fish. Additionally, non-specific physiological biomarkers, including hepatosomatic index and condition factor, were utilized to assess the environmental quality of the Curonian Lagoon. Fish samples were collected from two sites: the southern part of Klaipėda port, known as Kiaulės Nugara Island, and the central part of the Curonian Lagoon near Ventė Cape. Based on the multi-biomarker responses of C. gibelio, differences were observed between the two sampling sites, but not statistically significant. Consequently, C. gibelio cannot be recommended as a suitable bioindicator of environmental pollution and is not recommended for biomonitoring surveys of the Curonian Lagoon.