Polycyclic aromatic hydrocarbon exposure, miRNA genetic variations, and associated leukocyte mitochondrial DNA copy number: A cross-sectional study in China

Duan, Xiaoran; Yang, Yongli; Zhang, Hui; Liu, Bin; Wei, Wan; Wang, Liuya; Sun, Changqing; Yao, Wu; Cui, Liuxin; Zhou, Xiaoshan; Wang, Wei

doi:10.1016/j.chemosphere.2019.125773

Cited by 19 publications

(15 citation statements)

References 40 publications

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“…However, the mtDNAcn of all layers had signi cant differences between the two groups (P < 0.001). As reported in our early study [6], the results are shown in Supplementary Table 2.…”

Section: Individual Characteristics and Mtdnacnsupporting

confidence: 70%

“…Pieters et al (2013) demonstrated that mtDNAcn was inversely associated with indoor PAHs exposure population and their ndings were also con rmed in human TK6 cells [3]. Our previous study showed that mtDNAcn had signi cantly negative correlations with the levels of COE cumulative exposure dose [24]. Moreover, our previous study also found that mtDNAcn had signi cantly negative correlations with the levels of 1-OHPYR which can be used to estimate the internal exposure of PAHs.…”

Section: Discussionmentioning

confidence: 71%

“…Several studies have found that PAHs may interfere mitochondrial biosynthesis and further alter mitochondrial DNA copy number (mtDNAcn) [3][4][5]. Our previous study also found that mtDNAcn decreased with environmental PAHs-exposure [6].…”

Section: Introductionmentioning

confidence: 65%

“…The study protocol and consent form from all subjects were subjected to approval by the Ethics Committee of Zhengzhou University, China. More detailed information as described in our previous study [6].…”

Section: Study Population and Epidemiological Datamentioning

confidence: 99%

“…The β-globin gene was forward, 5'-GCTTCTGACACAACTGTGTTCACTAGC-3' and reverse, 5'-CACCAACTTC ATCCACGTTCACC-3'. Detailed information as described previously [6].…”

Section: Analysis Of Mtdnacnmentioning

confidence: 99%

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Genetic Polymorphisms of Metabolic Enzyme Genes Associated with Leukocyte Mitochondrial DNA Copy Number in PAHs Exposure Workers

Duan

Zhang

et al. 2020

Preprint

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Background: PAHs exposure had been reported to be a risk factor of mtDNAcn in our early study. However, the effect of metabolic enzymes’ genetic polymorphisms on mtDNAcn in PAHs-Exposure workers has not been fully evaluated.Methods: We investigated the effects of metabolic enzymes’ genetic polymorphisms on mtDNAcn among 544 coke oven workers and 238 office staffs. The mtDNAcn of peripheral blood leukocytes was measured using Real-time quantitative polymerase chain reaction method. Polymerase chain reaction and restriction fragment length was used to detect five polymorphisms in GSTT1, GSTM1, GSTP1 rs1695, CYP2E1 rs6413432, and CYP2E1 rs3813867.Results: The mtDNAcn in peripheral blood leukocytes was significantly lower in the exposure group than that in the control group (P < 0.001). The 1-OHPYR had an increasing trend with the genotypes AA→AG→GG of GSTP1 rs1695 in the control group. Generalized linear model indicated that the influencing factors of mtDNAcn were PAHs-exposure [b(95% CI) = -0.420 (-0.469, -0.372), P < 0.001], male [β(95% CI) = -0.058 (-0.103, -0.012), P = 0.013] ,and AA genotype for GSTP1 rs1695 [β(95% CI) = -0.051 (-0.095, -0.008), P = 0.020].Conclusions: The male was susceptibility to PAHs exposure. The AA genotype of GSTP1 rs1695 may influence the toxicity of PAHs and associated with the decreased of mtDNAcn.

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“…However, the mtDNAcn of all layers had signi cant differences between the two groups (P < 0.001). As reported in our early study [6], the results are shown in Supplementary Table 2.…”

Section: Individual Characteristics and Mtdnacnsupporting

confidence: 70%

Section: Discussionmentioning

confidence: 71%

Section: Introductionmentioning

confidence: 65%

Section: Study Population and Epidemiological Datamentioning

confidence: 99%

“…The β-globin gene was forward, 5'-GCTTCTGACACAACTGTGTTCACTAGC-3' and reverse, 5'-CACCAACTTC ATCCACGTTCACC-3'. Detailed information as described previously [6].…”

Section: Analysis Of Mtdnacnmentioning

confidence: 99%

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Genetic Polymorphisms of Metabolic Enzyme Genes Associated with Leukocyte Mitochondrial DNA Copy Number in PAHs Exposure Workers

Duan

Zhang

et al. 2020

Preprint

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Genetic polymorphisms of metabolic enzyme genes associated with leukocyte mitochondrial DNA copy number in PAHs exposure workers

Duan

Zhang

et al. 2021

Cancer Reports

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Background: Polycyclic aromatic hydrocarbons (PAHs) exposure had been reported to be a risk factor of mtDNAcn in our early study. However, the effect of metabolic enzymes' genetic polymorphisms on mtDNAcn in PAHs-Exposure workers has not been fully evaluated.Aim: The aim of the study was to explore the effect of metabolic enzymes' genetic polymorphisms on mtDNAcn in PAHs-Exposure. Methods and Results:We investigated the effects of metabolic enzymes' genetic polymorphisms on mtDNAcn among 544 coke oven workers and 238 office staffs.The mtDNAcn of peripheral blood leukocytes was measured using the Real-time quantitative polymerase chain reaction (PCR) method. PCR and restriction fragment length was used to detect five polymorphisms in GSTT1, GSTM1, GSTP1 rs1695, CYP2E1 rs6413432, and CYP2E1 rs3813867. The mtDNAcn in peripheral blood leukocytes was significantly lower in the exposure group than that in the control group (p < .001). The 1-OHPYR had an increasing trend with the genotypes AA!AG ! GG of GSTP1 rs1695 in the control group. Generalized linear model indicated that the influencing factors of mtDNAcn were PAHs-exposure [β (95% CI) = −0.420 (−0.469, −0.372), p < .001], male [β (95% CI) = −0.058 (−0.103, −0.012), p = .013], and AA genotype for GSTP1 rs1695 [β (95% CI) = −0.051 (−0.095, −0.008), p = .020]. Conclusion:The individuals carrying the AA genotype of GSTP1 rs1695 may have a lower mtDNAcn due to their weaker detoxification of PAHs.

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Circulating microRNAs as biomarkers of environmental exposure to polycyclic aromatic hydrocarbons: potential and prospects

Rani¹,

Kela²,

Dhaniya

et al. 2021

Environ Sci Pollut Res

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Polycyclic aromatic hydrocarbon exposure, miRNA genetic variations, and associated leukocyte mitochondrial DNA copy number: A cross-sectional study in China

Cited by 19 publications

References 40 publications

Genetic Polymorphisms of Metabolic Enzyme Genes Associated with Leukocyte Mitochondrial DNA Copy Number in PAHs Exposure Workers

Genetic Polymorphisms of Metabolic Enzyme Genes Associated with Leukocyte Mitochondrial DNA Copy Number in PAHs Exposure Workers

Genetic polymorphisms of metabolic enzyme genes associated with leukocyte mitochondrial DNA copy number in PAHs exposure workers

Circulating microRNAs as biomarkers of environmental exposure to polycyclic aromatic hydrocarbons: potential and prospects

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