Polycyclic aromatic hydrocarbon degradation and biosurfactant production by a newly isolated Pseudomonas sp. strain from used motor oil-contaminated soil

Chebbi, Alif; Hentati, Dorra; Zaghden, Hatem; Baccar, Nidhal; Rezgui, Fatma; Chalbi, Manel; Sayadi, Sami; Chamkha, Mohamed

doi:10.1016/j.ibiod.2017.05.006

Cited by 127 publications

(48 citation statements)

References 81 publications

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“…The oil displacement test (ODT) was used to evaluate the surface activity of supernatants and biosurfactants solutions. Crude oil (15 μL) was dropped to form a thin oil layer on the surface of the water (20 mL) into a Petri dish (diameter of 90 mm) and, therefore, 200 μL of a test solution was dropped onto the surface of oil as described elsewhere . The maximum diameter of the clear zone was measured.…”

Section: Methodsmentioning

confidence: 99%

“…Crude oil (15 L) was dropped to form a thin oil layer on the surface of the water (20 mL) into a Petri dish (diameter of 90 mm) and, therefore, 200 L of a test solution was dropped onto the surface of oil as described elsewhere. 21 The maximum diameter of the clear zone was measured. All values given are the mean of three replicates ± standard deviation.…”

Section: Surface Tension Measurement Emulsification Index (E24) and mentioning

confidence: 99%

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Promising abilities of mercapto‐degrading Staphylococcus capitis strain SH6 in both crude oil and waste motor oil as sole carbon and energy sources: its biosurfactant production and preliminary characterization

Chebbi

Hentati

Cheffi

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND It was shown previously that a Staphylococcus capitis strain SH6 was able to degrade several malodorous mercaptans and simultaneously reduce the surface tension. RESULTS This present work revealed the capacity of strain SH6 to grow on various hydrocarbons, used as the only carbon and energy sources. Based on GC–MS analyses, the substantial ability to degrade up to 45% and 64% of aliphatic hydrocarbons (n‐alkanes) of crude oil and waste motor oil, respectively, after 30 days of incubation at 37 °C and 180 rpm, was shown. The properties of biosurfactant produced by strain SH6 grown on different oil substrates (diesel oil and waste motor oil) were studied. Biosurfactants exhibited enhanced emulsification capacities and significant stabilities over a wide range of salinity (20–150 g L‐1), temperature (–20–100 °C), and pH , and also the ability to remove crude oil from contaminated soils. Their critical micelle concentrations (CMC) were of 800 mg L‐1. FTIR analyses suggested the lipopetide nature of biosurfactants. CONCLUSION The stabilities of biosurfactants over a wide pH range, high temperatures and variable concentrations of salt, as well as emulsifying properties, suggest potential applications in bioremediation processes. © 2017 Society of Chemical Industry

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Section: Methodsmentioning

confidence: 99%

Section: Surface Tension Measurement Emulsification Index (E24) and mentioning

confidence: 99%

Promising abilities of mercapto‐degrading Staphylococcus capitis strain SH6 in both crude oil and waste motor oil as sole carbon and energy sources: its biosurfactant production and preliminary characterization

Chebbi

Hentati

Cheffi

et al. 2018

J of Chemical Tech & Biotech

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“…NJ2 in comparison with P. aeruginosa PSA5 and O. intermedium P2, despite a higher bacterial growth,. Chebbi et al, (2017) demonstrated that the newly isolated Pseudomonas sp. strain from motor oil-contaminated soil was able to use both motor oil and n-hexadecane, among other hydrocarbons as a sole carbon and energy source.…”

Section: 1shake Flask Optimizationmentioning

confidence: 99%

“…hydrocarbons, such as phenanthrene through two possible degradation pathways: salicylate or protocatechuate (Chebbi et al, 2017). These pathways share the same common upper route and are initiated by the double hydroxylation of the aromatic ring.…”

Section: 4biodegradation Testsmentioning

confidence: 99%

Bench-scale production of enzymes from the hydrocarbonoclastic bacteria Alcanivorax borkumensis and biodegradation tests

Kadri

Magdouli

Rouissi

et al. 2018

Journal of Biotechnology

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This study investigates motor oil (3, 5, 7.5 and 10% (v v)) as a sole carbon source for the production of Alcanivorax borkumensis in shake flasks and a 5 L bench-scale fermenter in comparison to the standard media. Shake flask studies showed a significant and higher cell growth (p=0.000038), lipase (p = 0.006900) and alkane hydroxylase production (p = 0.000921) by Alcanivorax borkumensis when motor oil was used as the substrate. Based on Tukey post-hoc tests, 5% motor oil concentration was selected as the optimal substrate concentration. The 5 L fermenter experiments conducted using motor oil at 5% (v v) concentration, under controlled conditions exhibited significant and higher alkane hydroxylase and lipase activities (55.6 U mL (p = 0.018418) and 208.30 U mL (p = 0.020087), respectively) as compared with those of motor oil at 3% (v v) and n-hexadecane at 3% (v v) concentration which was used as control. Cell growth was significantly higher when motor oil (3 or 5%) was used as a substrate (p = 0.024705). Enzymatic degradation tested on two different polycyclic aromatic hydrocarbons (PAHs) contaminated groundwaters showed 37.4% removal after 5 days with a degradation rate of 196.6 ppb day and 82.8% removal after 10 days with a degradation rate of 217.54 ppb day for the 1st site and an almost complete biodegradation with 95% removal and 499.02 ppb day removal rate after only 5 days for the 2nd site.

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“…Degradations of aromatic compounds using bacteria of the Pseudomonas gender are commonly mentioned, such as Pseudomonas putida (Ridgway et al, 1990;Lee et al, 1994;Otenio et al, 2005;Shim et al, 2005;Lin et al, 2010); Pseudomonas aeruginosa (Ridgway et al, 1990;, Nwinyi et al, 2016;Chebbi et al, 2017;Khodaei et al, 2017); Pseudomonas fluorescens (Ridgway et al, 1990;Shim et al, 2005;Oberoi and Philip, 2017); Pseudomonas aureofaciens (Dou et al 2008); Pseudomonas stutzeri (Singh and Tiwary, 2017); Pseudomonas plecoglossicida (Li et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

KINETICS OF THE BIODEGRADATION OF MONOAROMATICS BY Pseudomonas aeruginosa

Câmara

Sousa

Neto

2019

Braz. J. Chem. Eng.

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Water contamination by monoaromatic compounds has risen throughout time, which leads to the necessity of developing new water treatment technology, capable of minimizing their negative effect on the environment. In this context, biological processes present themselves as a solution to the processes of extraction. Bioremediation makes use of microbial groups capable of using hydrocarbons as a source of carbon to perform their metabolic functions. This work evaluated the biodegradation efficiency of Pseudomonas aeruginosa strain isolated from contaminated matrices, for the substrates benzene, ethylbenzene and toluene, aiming to determine to which compound the bacteria had better adaptation. For that, bioremediation assays were performed for each of the monoaromatic compounds, in an isolated way, with the goal of obtaining experimental data and from this Monod and Andrews kinetic models were discretized and numerically developed through the Runge-Kutta method. It was possible to observe that Pseudomonas aeruginosa has a bigger affinity for ethylbenzene, while benzene generated a bigger microbian coefficient. Monod´s model was capable of predicting satisfactorily the experimental data.

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Polycyclic aromatic hydrocarbon degradation and biosurfactant production by a newly isolated Pseudomonas sp. strain from used motor oil-contaminated soil

Cited by 127 publications

References 81 publications

Promising abilities of mercapto‐degrading Staphylococcus capitis strain SH6 in both crude oil and waste motor oil as sole carbon and energy sources: its biosurfactant production and preliminary characterization

Promising abilities of mercapto‐degrading Staphylococcus capitis strain SH6 in both crude oil and waste motor oil as sole carbon and energy sources: its biosurfactant production and preliminary characterization

Bench-scale production of enzymes from the hydrocarbonoclastic bacteria Alcanivorax borkumensis and biodegradation tests

KINETICS OF THE BIODEGRADATION OF MONOAROMATICS BY Pseudomonas aeruginosa

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