Polyclonal antibodies against O6-methylguanine-DNA methyltransferase in adapted bacteria

Ceccoli, Joseph; Rosales, Nestor; Goldstein, Mindy S.; Yarosh, Daniel B.

doi:10.1016/0167-8817(88)90023-5

Cited by 17 publications

(7 citation statements)

References 20 publications

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“…The inducible syntheses of the repair enzymes make the cells resistant to the lethal and mutagenic effects of MNNG. This response is not universally distributed, but seems to be confined to several prokaryotic species (4,13,14). We showed that Bacillus subtilis cells exhibit a similar response to alkylation; however, several differences between the E. coli and B. subtilis system have been noted (15)(16)(17)(18)(19).…”

Section: Introductionmentioning

confidence: 68%

Bacillus subtilis adaoperon encodes two DNA alkyltransferases

Morohoshi¹,

Hayashi²,

Munakata³

1990

Nucl Acids Res

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By prophage transformation and subcloning, we have obtained Bacillus subtilis DNA fragments that could complement the hypersensitivity of ada (adaptive response deficient) mutants to N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The nucleotide sequence contained two open reading frames that were assigned to the genes adaA and adaB, encoding methylphosphotriester-DNA methyltransferase and O6-methylguanine-DNA methyltransferase, respectively. These two genes overlap by 11 bp and comprise a small operon. The 1.6 Kb transcripts derived from the operon were detected in ada+ cells cultured in the presence of MNNG but not in control ada+ cells. From analysis of the syntheses of DNA alkyltransferases in the ada mutant cells harboring the plasmid carrying the complete or partial fragment, we conclude that the adaA gene product functions as a transcriptional activator of the ada operon, while the adaB gene product specializes in repair of mutagenic O6-methylguanine residues. Comparison with Escherichia coli ada operon showed that the two genes correspond to portions of the E. coli ada gene, implicating gene fusion or splitting as the origin of the difference in the organizations of the genes.

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Section: Introductionmentioning

confidence: 68%

Bacillus subtilis adaoperon encodes two DNA alkyltransferases

Morohoshi¹,

Hayashi²,

Munakata³

1990

Nucl Acids Res

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“…Adaptive repair is induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in several enterobacterial species (39), Bacillus subtilis (21,22), Bacillus thuringiensis (3), Micrococcus luteus (1), Streptomyces fradiae (2), Rhodobacter sphaeroides (40), Rhodobacter capsulatus (40), Pseudomonas aeruginosa (8), and Xanthomonas maltophilia (8). On the other hand, a similar DNA repair system has not been found in Haemophilus influenzae (16), Salmonella typhimurium (11,39,43), Neisseria gonorrhoeae (6), Rhizobium meliloti (15), or Staphylococcus au- reus (8), although a weak induction of a 39-kDa protein immunologically related to the E. coli Ada protein has been detected in S. typhimurium (39). Nevertheless, the presence of Ada-like proteins in several genera of gram-negative bacteria which were able to recognize the Ada box of E. coli has not been analyzed.…”

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confidence: 99%

Induction of the alkA gene of Escherichia coli in gram-negative bacteria

Henestrosa

Barbé

1991

J Bacteriol

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A broad-host-range plasmid containing a fusion of the alkA and lacZ genes of Escherichia coil was introduced into various aerobic and facultative gram-negative bacteria-33 species belonging to 19 genera-to study the induction of expression of the alkA gene by alkylating agents. The bacteria included species of the families Enterobacteriaceae, Pseudomonadaceae, Rhizobiaceae, Vibrionaceae, Neisseriaceae, Rhodospirillaceae, and Azotobacteraceae. Results obtained show that all bacteria tested, except Aeromonas hydrophila, Agrobacterium tumefaciens, Hafnia alvei, Rhizobium meliloti, Salmonella enteritidis, Xanthomonas campestris, and those of the genus Rhodobacter, are able to induce the alkA gene of E. coli in the presence of N-methyl-N'-nitro-Nnitrosoguanidine. All these data indicate that the adaptive response to alkylating agents is present in bacterial species of several families and that the Ada box sequence must be widely conserved.

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“…Up to date there are only a few published reports about alkylation damage repair in P . aeruginosa [70,71]. However, it is known that P .…”

Section: Discussionmentioning

confidence: 99%

DNA Polymerases ImuC and DinB Are Involved in DNA Alkylation Damage Tolerance in Pseudomonas aeruginosa and Pseudomonas putida

et al. 2017

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Translesion DNA synthesis (TLS), facilitated by low-fidelity polymerases, is an important DNA damage tolerance mechanism. Here, we investigated the role and biological function of TLS polymerase ImuC (former DnaE2), generally present in bacteria lacking DNA polymerase V, and TLS polymerase DinB in response to DNA alkylation damage in Pseudomonas aeruginosa and P. putida. We found that TLS DNA polymerases ImuC and DinB ensured a protective role against N- and O-methylation induced by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in both P. aeruginosa and P. putida. DinB also appeared to be important for the survival of P. aeruginosa and rapidly growing P. putida cells in the presence of methyl methanesulfonate (MMS). The role of ImuC in protection against MMS-induced damage was uncovered under DinB-deficient conditions. Apart from this, both ImuC and DinB were critical for the survival of bacteria with impaired base excision repair (BER) functions upon alkylation damage, lacking DNA glycosylases AlkA and/or Tag. Here, the increased sensitivity of imuCdinB double deficient strains in comparison to single mutants suggested that the specificity of alkylated DNA lesion bypass of DinB and ImuC might also be different. Moreover, our results demonstrated that mutagenesis induced by MMS in pseudomonads was largely ImuC-dependent. Unexpectedly, we discovered that the growth temperature of bacteria affected the efficiency of DinB and ImuC in ensuring cell survival upon alkylation damage. Taken together, the results of our study disclosed the involvement of ImuC in DNA alkylation damage tolerance, especially at low temperatures, and its possible contribution to the adaptation of pseudomonads upon DNA alkylation damage via increased mutagenesis.

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Polyclonal antibodies against O6-methylguanine-DNA methyltransferase in adapted bacteria

Cited by 17 publications

References 20 publications

Bacillus subtilis adaoperon encodes two DNA alkyltransferases

Bacillus subtilis adaoperon encodes two DNA alkyltransferases

Induction of the alkA gene of Escherichia coli in gram-negative bacteria

DNA Polymerases ImuC and DinB Are Involved in DNA Alkylation Damage Tolerance in Pseudomonas aeruginosa and Pseudomonas putida

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