Polycaprolactone/silk fibroin electrospun nanofibers‐based lateral flow test strip for quick and facile determination of bisphenol A in breast milk

Abstract

This study aimed to develop a sensitive lateral flow test strip for the detection of bisphenol A (BPA) in breast milk. Conventional nitrocellulose test membrane was coated with the coaxial nanofiber, consisting of the inner polycaprolactone (PCL) and the outer PCL/silk fibroin (SF) mixture, to decrease the flow rate of the breast milk in the lateral flow assay (LFA). The nanofiber was prepared by using coaxial electrospinning, and BPA antibody was immobilized physically to the nanofiber. This nanofiber was used as a test membrane in the LFA. Color changes on the test membrane were evaluated as the signal intensity of the BPA. Breast milk creates a background on surfaces due to its structural properties. This background was detected by comparing the signal intensity with the signal intensity of water. The higher signal intensity was found in water samples when compared to breast milk samples. Although the detection limit is 2 ng/ml in both coaxial PCL/SF nanofiber and nitrocellulose (NC) test membranes, the color intensity increased with the increasing BPA concentration in the coaxial PCL/SF nanofiber. As a new dimension, the coaxial PCL/SF nanofiber provided higher color intensity than the NC membrane. In conclusion, a sensitive onsite method was developed for the detection of BPA in breast milk by using new coaxial PCL/SF nanofiber as a test membrane in LFA.

Lateral Flow Assay: A Summary of Recent Progress for Improving Assay Performance

“…The authors achieved an eight-fold enhancement in the LOD for human chorionic gonadotropin measurement compared to the conventional LFA [ 28 ]. Other published works in this field employed cellulose fibers as the sample pad, creating a wall before the test line region onto the nitrocellulose film, and the use of chitosan and nanofiber on nitrocellulose membrane [ 29 , 50 , 51 ]. All these approaches can slow the flow rate and modify the sensitivity of recognition from one to multiple folds in LFA.…”

Optical sensors based on electrospun membranes – principles, applications, and prospects for chemistry and biology

“…In some OSBEMs coaxial nanofibers are used instead of the ordinary ones. 84,157 For example, an enzymatic glucose sensor based was developed by adsorbing glucose oxidase onto the outer (shell) fibers. 84 The inner fibers contained an oxygen transducer PdTFPP, which increased luminescence in response to glucose oxidation.…”