Polycaprolactone scaffold modified with galactosylated chitosan for hepatocyte culture

Qiu, Yuan; Mao, Zhengwei; Zhao, Yimu; Zhang, Jiaheng; Guo, Qi; Gou, Zhongru; Gao, Changyou

doi:10.1007/s13233-012-0056-8

Cited by 23 publications

(17 citation statements)

References 36 publications

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“…After being cultured for 7 days, the cell‐seeded scaffolds were fixed in 4% glutaraldehyde solution and dehydrated in the gradient water/ethanol solutions and tert ‐butanol/ethanol solutions (volume ratios of tert ‐butanol are 30, 50, 70, 80, 90, and 100% respectively), then dried by lyophilization. The cross‐sectional morphology of the cell‐seeded scaffolds was observed under SEM after a thin gold layer was coated . Moreover, the BMSCs cultured in the scaffolds for 7 and 14 days were stained by fluorescein diacetate (FDA) according to a method reported in the literature and observed under a confocal laser scanning microscope (CLSM, Leica SP5, Germany).…”

Section: Methodsmentioning

confidence: 99%

“…The cross-sectional morphology of the cell-seeded scaffolds was observed under SEM after a thin gold layer was coated. 29 Moreover, the BMSCs cultured in the scaffolds for 7 and 14 days were stained by fluorescein diacetate (FDA) according to a method reported in the literature 30 and observed under a confocal laser scanning microscope (CLSM, Leica SP5, Germany). The cell viability was measured by an MTT (3-(4,5-dimethyl)thiazol-2-y1-2,5-dimethyl tetrazolium bromide, Sigma) assay.…”

Section: Cell Morphology and Viabilitymentioning

confidence: 99%

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Preparation of polycaprolactone microspheres‐aggregated scaffold with ultra big pores and fuzzy sphere surface by a one‐step phase separation method

Wang¹,

Ma²,

Li³

et al. 2013

J Biomedical Materials Res

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A microspheres-aggregated scaffold with ultra big pores (over 300 μm) and fuzzy microspheres is fabricated by incubating polycaprolactone (PCL)/tetrahydrofuran (THF) solution in a -20°C refrigerator, following by freeze-drying. Formation of the scaffold is mainly governed by the crystallization of the PCL polymer at appropriate conditions. All the 10-20% PCL/THF solutions yield the microspheres-aggregated scaffolds when the initial solution temperature is higher than 37°C, whereas the 10-15% solutions form dense membranes when the initial solution temperature is below 25°C. The size of the microspheres and pores is as large as 70-150 μm and 170-816 μm, respectively. The PCL microspheres-aggregated scaffold can better support the adhesion and proliferation of bone marrow mesenchymal stem cells (BMSCs) compared to the traditional porous scaffold obtained by a porogen leaching method. The tendencies of chondrogenesis and osteogenesis differentiation of BMSCs are observed on the microspheres-aggregated scaffold and the ordinary porous scaffold, respectively.

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Section: Methodsmentioning

confidence: 99%

Section: Cell Morphology and Viabilitymentioning

confidence: 99%

Preparation of polycaprolactone microspheres‐aggregated scaffold with ultra big pores and fuzzy sphere surface by a one‐step phase separation method

Wang¹,

Ma²,

Li³

et al. 2013

J Biomedical Materials Res

Self Cite

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“…Such cultures were even faster than collagen sandwich cultures in the rate of tissue formation [198]. Qui et al modified PCL scaffold with galactosylated chitosan and showed an improved hepatic functionality over a period of 7 days as compared to a 2D monolayer or PCL 3D scaffold [199].…”

Section: Hybrid Biomaterials Applied For Livermentioning

confidence: 99%

Polymer- and Hybrid-Based Biomaterials for Interstitial, Connective, Vascular, Nerve, Visceral and Musculoskeletal Tissue Engineering

2020

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In this review, materials based on polymers and hybrids possessing both organic and inorganic contents for repairing or facilitating cell growth in tissue engineering are discussed. Pure polymer based biomaterials are predominantly used to target soft tissues. Stipulated by possibilities of tuning the composition and concentration of their inorganic content, hybrid materials allow to mimic properties of various types of harder tissues. That leads to the concept of "one-matches-all" referring to materials possessing the same polymeric base, but different inorganic content to enable tissue growth and repair, proliferation of cells, and the formation of the ECM (extra cellular matrix). Furthermore, adding drug delivery carriers to coatings and scaffolds designed with such materials brings additional functionality by encapsulating active molecules, antibacterial agents, and growth factors. We discuss here materials and methods of their assembly from a general perspective together with their applications in various tissue engineering sub-areas: interstitial, connective, vascular, nervous, visceral and musculoskeletal tissues. The overall aims of this review are two-fold: (a) to describe the needs and opportunities in the field of bio-medicine, which should be useful for material scientists, and (b) to present capabilities and resources available in the area of materials, which should be of interest for biologists and medical doctors.Polymers 2020, 12, 620 2 of 30 allows to tailor functionalize the coatings, where their responsiveness to external stimuli is one of their biggest advantages.Polymers stand out as a special class of materials due to the flexibility of design of their blocks and various functionalities, which is brought by their versatile assembly or by introducing additional side-groups or blocks, in the case of block-co-polymers. In this regard, both synthetic and biocompatible polymers are available, and polymer engineering represents a growing area tailoring the needs often driven by applications. With all advantages and the flexibility of the design, there is one significant weakness of polymers-the softness. In regard with tissue engineering, that translates to the fact that some materials can match predominantly soft tissue, but it is difficult to tune mechanical properties of polymers to match the hardness of bones, tendons, etc. And since mechanical properties of materials affect and even drive cell and tissue growth, enhancement of mechanical properties of polymers and biomaterials is essential. Chemical crosslinking can be applied to address these challenges, but that solves problems only partially.To solve these challenges the so-called hybrid materials [6] are used, which possess both inorganic and organic contents. Increasingly, hybrid materials are used in designing the extracellular matrix. The hybrid matrix design for various tissue engineering applications should meet bio-medical requirements. On the one hand, it needs to be biocompatible and biodegradable, which is achieved through the po...

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“…Furthermore, in vitro tests have been performed to investigate on hepatocytes homeostasis on this type of substrates. Contextually, Qui et al discussed a preparation of mixed scaffolds made of galactosylated chitosan and polycaprolactone (PCL) [61]. In this paper, PCL scaffolds-prepared by a method of gelatin particle leaching-were hydrolyzed to produce carboxylic groups that were employed to react with the amino groups of the galactosylated chitosan.…”

Section: Glycosylated-chitosan Derivatives For Tissue Engineeringmentioning

confidence: 99%

Glycosylated-Chitosan Derivatives: A Systematic Review

et al. 2020

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Chitosan derivatives, and more specifically, glycosylated derivatives, are nowadays attracting much attention within the scientific community due to the fact that this set of engineered polysaccharides finds application in different sectors, spanning from food to the biomedical field. Overcoming chitosan (physical) limitations or grafting biological relevant molecules, to mention a few, represent two cardinal strategies to modify parent biopolymer; thereby, synthetizing high added value polysaccharides. The present review is focused on the introduction of oligosaccharide side chains on the backbone of chitosan. The synthetic aspects and the effect on physical-chemical properties of such modifications are discussed. Finally, examples of potential applications in biomaterials design and drug delivery of these novel modified chitosans are disclosed.

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Polycaprolactone scaffold modified with galactosylated chitosan for hepatocyte culture

Cited by 23 publications

References 36 publications

Preparation of polycaprolactone microspheres‐aggregated scaffold with ultra big pores and fuzzy sphere surface by a one‐step phase separation method

Preparation of polycaprolactone microspheres‐aggregated scaffold with ultra big pores and fuzzy sphere surface by a one‐step phase separation method

Polymer- and Hybrid-Based Biomaterials for Interstitial, Connective, Vascular, Nerve, Visceral and Musculoskeletal Tissue Engineering

Glycosylated-Chitosan Derivatives: A Systematic Review

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