Polycaprolactone/glass bioabsorbable implant in a rabbit humerus fracture model

Lowry, Kent Jason; Hamson, K. R.; Bear, L.; Peng, Y. B.; Calaluce, Robert; Evans, M. L.; Anglen, Jeff; Allen, William C.

doi:10.1002/(sici)1097-4636(19970915)36:4<536::aid-jbm12>3.3.co;2-v

Cited by 15 publications

(20 citation statements)

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“…As a consequence of this interest, a variety of hydrolytically degradable polymers have been developed or applied in the field. The majority of these polymers consist of high‐molecular‐weight linear aliphatic polyesters and their copolymers and possess mechanical properties best suited for orthopedic tissue engineering 1–5…”

Section: Introductionmentioning

confidence: 99%

Synthesis, characterization, and cytocompatibility of elastomeric, biodegradable poly(ester‐urethane)ureas based on poly(caprolactone) and putrescine

Guan

Sacks

Beckman

et al. 2002

J. Biomed. Mater. Res.

288

290

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The engineering of tissue for mechanically demanding applications in the cardiovascular system is likely to require mechanical conditioning of cell-scaffold constructs prior to their implantation. Scaffold properties amenable to such an application include high elasticity and strength coupled with controllable biodegradative and cell-adhesive properties. To fulfill such design criteria, we have synthesized a family of poly(ester-urethane)ureas (PEUUs) from polycaprolactone and 1,4-diisocyanatobutane. Lysine ethyl ester (Lys) or putrescine was used as chain extenders. To encourage cell adhesion, PEUUs were surface modified with radio-frequency glow discharge followed by coupling of Arg-Gly-Asp-Ser (RGDS). The synthesized PEUUs were highly flexible, with breaking strains of 660-895% and tensile strengths from 9.2-29 MPa. Incubation in aqueous buffer for 8 weeks resulted in mass loss, from >50% (Lys chain extender) to 10% (putrescine chain extender). Human endothelial cells cultured for 4 days with medium containing the degradation products from PEUUs with either the Lys or putrescine chain extender showed no toxic effects. Cell adhesion was 85% of that measured on tissue-culture polystyrene for unmodified PEUU surfaces (p < 0.01) and >160% (p < 0.001) of polystyrene on RGDS-modified PEUUs. These biodegradable PEUUs demonstrate potential for future application as cell scaffolds in cardiovascular tissue-engineering or other soft-tissue applications.

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Section: Introductionmentioning

confidence: 99%

Synthesis, characterization, and cytocompatibility of elastomeric, biodegradable poly(ester‐urethane)ureas based on poly(caprolactone) and putrescine

Guan

Sacks

Beckman

et al. 2002

J. Biomed. Mater. Res.

288

290

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“…Similar indications of the biocompatibility of PCL findings have been reported in other studies. [29][30][31] In particular, PCL microspheres have been shown to induce low levels of neutrophil activation and a mild inflammatory response in the early days of implantation, which disappeared after 3 months. 29 In contrast poly(lactide), which has been widely investigated for production of biomedical implants, drug delivery systems, and fibers, has been reported to provoke chronic inflammation with increases in multinuclear giant cells and macrophages engulfing material and inhibiting bone formation.…”

Section: Discussionmentioning

confidence: 99%

Gravity Spun Polycaprolactone Fibers for Applications in Vascular Tissue Engineering: Proliferation and Function of Human Vascular Endothelial Cells

Williamson

Woollard²,

Griffiths³

et al. 2006

Tissue Engineering

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Poly(epsilon-caprolactone) (PCL) fibers produced by wet spinning from solutions in acetone under lowshear (gravity-flow) conditions resulted in fiber strength of 8 MPa and stiffness of 0.08 Gpa. Cold drawing to an extension of 500% resulted in an increase in fiber strength to 43 MPa and stiffness to 0.3 GPa. The growth rate of human umbilical vein endothelial cells (HUVECs) (seeded at a density of 5 x 10(4) cells/mL) on as-spun fibers was consistently lower than that measured on tissue culture plastic (TCP) beyond day 2. Cell proliferation was similar on gelatin-coated fibers and TCP over 7 days and higher by a factor of 1.9 on 500% cold-drawn PCL fibers relative to TCP up to 4 days. Cell growth on PCL fibers exceeded that on Dacron monofilament by at least a factor of 3.7 at 9 days. Scanning electron microscopy revealed formation of a cell layer on samples of cold-drawn and gelatin-coated fibers after 24 hours in culture. Similar levels of ICAM-1 expression by HUVECs attached to PCL fibers and TCP were measured using RT-PCR and flow cytometry, indicative of low levels of immune activation. Retention of a specific function of HUVECs attached to PCL fibers was demonstrated by measuring their immune response to lipopolysaccharide. Levels of ICAM-1 expression increased by approximately 11% in cells attached to PCL fibers and TCP. The high fiber compliance, favorable endothelial cell proliferation rates, and retention of an important immune response of attached HUVECS support the use of gravity spun PCL fibers for three-dimensional scaffold production in vascular tissue engineering.

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“…It biocompatibility is similar to PLA and PGA. However, it has lower degradation rate as compared to PLA and PGA (Lowry et al 1997). The degradation rate of PCL is more than 24 months (Woodruff and Hutmacher 2010) and makes it less attractive for general tissue engineering applications but it acts as a better long-term drug delivery carrier.…”

Section: Polycaprolactone (Pcl)mentioning

confidence: 99%