Polybutylene terephthalate-based stationary phase for ion-pair-free reversed-phase liquid chromatography of small interfering RNA. Part 1: Direct coupling with mass spectrometry

Li, Feiyang; Chen, Shenkai; Studzińska, Sylwia; Lämmerhofer, Michael

doi:10.1016/j.chroma.2023.463898

Cited by 6 publications

(1 citation statement)

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“…Consequently, recent publications highlight the advantages of employing 2D-LC-MS for the comprehensive characterization of these ONs. In the two dimensions of this approach, various chromatographic modes have been utilized, including ion-pairing reversed-phase liquid chromatography (IP-RPLC), HILIC, and anion exchange chromatography (AEX) [77][78][79].…”

Section: Application Of Md-lc To Emerging Molecule Formatsmentioning

confidence: 99%

Multi‐dimensional technology – Recent advances and applications for biotherapeutic characterization

Bouvarel,

Camperi,

Guillarme

2024

J of Separation Science

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This review provides an overview of the latest advancements and applications in multi‐dimensional liquid chromatography coupled with mass spectrometry (mD‐LC‐MS), covering aspects such as inter‐laboratory studies, digestion strategy, trapping column, and multi‐level analysis. The shift from an offline to an online workflow reduces sample processing artifacts, analytical variability, analysis time, and the labor required for data acquisition. Over the past few years, this technique has demonstrated sufficient maturity for application across a diverse range of complex products. Moreover, there is potential for this strategy to evolve into an integrated process analytical technology tool for the real‐time monitoring of monoclonal antibody quality. This review also identifies emerging trends, including its application to new modalities, the possibility of evaluating biological activity within the mD‐LC set‐up, and the consideration of multi‐dimensional capillary electrophoresis as an alternative to mD‐LC. As mD‐LC‐MS continues to evolve and integrate emerging trends, it holds the potential to shape the next generation of analytical tools, offering exciting possibilities for enhanced characterization and monitoring of complex biopharmaceutical products.

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Section: Application Of Md-lc To Emerging Molecule Formatsmentioning

confidence: 99%