Polybrene Neutralization as a Rapid Means of Monitoring Blood Heparin Levels

Grann, Victor R.; Homewood, Kathryn; Golden, Walter R.C.

doi:10.1093/ajcp/58.1.26

Cited by 19 publications

(10 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The dosage of hexadimethrine bromide used was 0.0085 mg/U of heparin. 23 A solution of 75 µl of 3.2% sodium citrate was added to a series of test tubes for collecting blood samples. The control sample was obtained by adding 2 µl of the radioactive PF4 solution to a 0.5 ml blood sample drawn prior to the experiment.…”

Section: Inhibition Of Collagenase Activitymentioning

confidence: 99%

In Vivo Studies on the Binding of Heparin and Its Fractions with Platelet Factor 4

Walz¹,

Hung²

1985

Semin Thromb Hemost

View full text Add to dashboard Cite

PF4 has a half-life in plasma of less than 3 minutes, and its rapid clearance appears to be a function of binding to the vascular endothelium. Once bound to the endothelium, PF4 can be released by heparin in a time-dependent manner; recovery is greater the sooner heparin is administered following PF4 infusion. This heparin-induced release of PF4 can be abolished if the heparin is first complexed with hexadimethrine bromide. Likewise, this heparin-induced release of PF4 is dependent upon the type of heparin used; low molecular weight heparin fractions and fragments do not cause the PF4 rebound seen with intact heparin. Thus, it would appear that low molecular weight forms of heparin are advantageous in that their in vivo administration would not be mediated by such platelet modulators as PF4.

show abstract

Section: Inhibition Of Collagenase Activitymentioning

confidence: 99%

In Vivo Studies on the Binding of Heparin and Its Fractions with Platelet Factor 4

Walz¹,

Hung²

1985

Semin Thromb Hemost

View full text Add to dashboard Cite

show abstract

“…Polybrene is a positively charged quaternary nitrogen poly mer, used as a safe alternative heparin neu tralizer in patients with a protamine allergy [12,13], in spite of its reported nephrotoxicity [14], Polybrene is mainly used for in vitro heparin titration experiments [15,16]. In the late 60s, several articles reported polybrene to be superior to protamine [17][18][19].…”

Section: Introductionmentioning

confidence: 99%

Influence of Heparin, Protamine and Polybrene on the Time Integral of Thrombin Generation (Endogenous Thrombin Potential)

Rotteveel¹,

Roozendaal

Weijers³

et al. 1996

Pathophysiol Haemos Thromb

View full text Add to dashboard Cite

We investigated the anticoagulating and heparin-neutralizing properties of protamine and polybrene (hexadimethrine bromide), using the endogenous thrombin potential (ETP) as the parameter to access plasma coagulability. The hypocoagulability induced by high doses of heparin (3 IU/ml) could be reversed by addition of protamine to a very limited extent only. Polybrene on the other hand did neutralize heparin at the equivalent concentration and a two-fold excess did not influence the ETP parameters. In vivo neutralization of high-dose heparin with protamine should therefore be reconsidered. Our experiments suggest polybrene to be superior over protamine with respect to neutralization of high doses of heparin.

show abstract

“…Coagulation profile: activated partial thromboplastin time (Morin & Willoughby, 1975) using fibrolet (activated platelet factor reagent, BBL, Div. Becton Dickinson Co., Cockeysville, Md., 21030, U.S.A.) and Fibrometer; Quick's one-stage prothrombin time (Quick, 1966) using rabbit brain and lung thrombo plastin (Automated Simplastin, General Diagnostic Div., Warner, Lambert Co., Morris Plains, New Jersey, U.S.A.), and thrombin time (Grann et at., 1972) using bovine topical thrombin (Parke-Davis). Serial dilution protamine sulphate (SDPS) test (Gurrewich & Hutchinson, 1971) was done where applicable.…”

Section: Methodsmentioning

confidence: 99%

Experience with the management of deep vein thrombosis in patients with spinal cord injury

Perkash

1980

Spinal Cord

View full text Add to dashboard Cite

Polybrene Neutralization as a Rapid Means of Monitoring Blood Heparin Levels

Cited by 19 publications

References 0 publications

In Vivo Studies on the Binding of Heparin and Its Fractions with Platelet Factor 4

In Vivo Studies on the Binding of Heparin and Its Fractions with Platelet Factor 4

Influence of Heparin, Protamine and Polybrene on the Time Integral of Thrombin Generation (Endogenous Thrombin Potential)

Experience with the management of deep vein thrombosis in patients with spinal cord injury

Contact Info

Product

Resources

About