Polybenzimidazole nanofibers for neural stem cell culture

Garrudo, Fábio F. F.; Udangawa, Ranodhi N.; Hoffman, Pauline R.; Sordini, Laura; Chapman, Caitlyn A.; Mikael, Paiyz E.; Ferreira, Flávio Alves; Silva, João Carlos; Rodrigues, Carlos Alberto Chirano; Cabral, Joaquim M. S.; Morgado, Jorge; Ferreira, Frederico Castelo; Linhardt, Robert J.

doi:10.1016/j.mtchem.2019.08.004

Cited by 20 publications

(16 citation statements)

References 61 publications

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“…The fibers of neat PBI are randomly oriented, homogeneous, showing few defects, and have an average diameter of 184 ± 59 nm. This average diameter value is similar to that obtained by Jahangiri et al [31], but slightly larger than the values obtained in previous works [26,32], which we attribute to the milder electrospinning conditions used in the present study, namely the lower voltage and tip-to-collector distance. After coating, the diameter of the fibers increased to 256 ± 59 nm and 279 ± 54 nm, for spin coated and dip coated fibers, respectively.…”

Section: Morphological Characterization Of Electrospun Scaffoldssupporting

confidence: 89%

“…The peak at 1618 cm −1 corresponds to C = N stretching, also present in the imidazole groups. The region between 2400 and 3955 cm −1 is attributed to amine groups, widely distributed through the polymer’s structure [ 26 , 37 ]. The presence of the solvent used for electrospinning, DMAc, was not detected.…”

Section: Resultsmentioning

confidence: 99%

“…PBI is rarely used in the context of medical applications and therefore it is not an obvious choice as carrier electrospun fibers to be coated with PEDOT:PSS. A recent study by our group has demonstrated, for the first time, a superior biocompatibility property of polybenzimidazole (PBI) electrospun nanofibers to support neural stem cell culture [ 26 ]. In our study [ 26 ], we were able to increase PBI nanofiber conductivity through doping with different acids.…”

Section: Introductionmentioning

confidence: 99%

“…A recent study by our group has demonstrated, for the first time, a superior biocompatibility property of polybenzimidazole (PBI) electrospun nanofibers to support neural stem cell culture [ 26 ]. In our study [ 26 ], we were able to increase PBI nanofiber conductivity through doping with different acids. Nonetheless, the highest value obtained was far below 1 S·m −1 .…”

Section: Introductionmentioning

confidence: 99%

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PEDOT:PSS-Coated Polybenzimidazole Electroconductive Nanofibers for Biomedical Applications

et al. 2021

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Bioelectricity drives several processes in the human body. The development of new materials that can deliver electrical stimuli is gaining increasing attention in the field of tissue engineering. In this work, novel, highly electrically conductive nanofibers made of poly [2,2′-m-(phenylene)-5,5′-bibenzimidazole] (PBI) have been manufactured by electrospinning and then coated with cross-linked poly (3,4-ethylenedioxythiophene) doped with poly (styrene sulfonic acid) (PEDOT:PSS) by spin coating or dip coating. These scaffolds have been characterized by scanning electron microscopy (SEM) imaging and attenuated total reflectance Fourier-transform infrared (ATR-FTIR) spectroscopy. The electrical conductivity was measured by the four-probe method at values of 28.3 S·m−1 for spin coated fibers and 147 S·m−1 for dip coated samples, which correspond, respectively, to an increase of about 105 and 106 times in relation to the electrical conductivity of PBI fibers. Human bone marrow-derived mesenchymal stromal cells (hBM-MSCs) cultured on the produced scaffolds for one week showed high viability, typical morphology and proliferative capacity, as demonstrated by calcein fluorescence staining, 4′,6-diamidino-2-phenylindole (DAPI)/Phalloidin staining and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide] assay. Therefore, all fiber samples demonstrated biocompatibility. Overall, our findings highlight the great potential of PEDOT:PSS-coated PBI electrospun scaffolds for a wide variety of biomedical applications, including their use as reliable in vitro models to study pathologies and the development of strategies for the regeneration of electroactive tissues or in the design of new electrodes for in vivo electrical stimulation protocols.

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Section: Morphological Characterization Of Electrospun Scaffoldssupporting

confidence: 89%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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PEDOT:PSS-Coated Polybenzimidazole Electroconductive Nanofibers for Biomedical Applications

et al. 2021

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“…Among the electroconductive materials available to produce such platforms, the most used are metals and organic materials, namely graphene, and conjugated polymers. Conjugated polymers present numerous advantages toward neural tissue engineering applications, namely: (1) they can be processed into any desired 3D-shape (Garrudo et al, 2019a , b ; Kayser and Lipomi, 2019 ); (2) can be easily functionalized allowing the tailoring of their mechanical, chemical, and electrical properties; (3) can exhibit high electroconductivity values, approaching those of metals; and (4) combine ionic and electronic conductivity, improving the “quality” of the interfaces with biological tissues (Rivnay et al, 2014 ; Inal et al, 2018 ; Goel et al, 2019 ; Pace et al, 2019 ). While electronic conductivity promotes higher current flow across the material, ion conductivity may prove to be essential to interface with tissues, as electrical signals in the human body are predominantly associated to ion currents.…”

Section: Introductionmentioning

confidence: 99%

Effect of Electrical Stimulation Conditions on Neural Stem Cells Differentiation on Cross-Linked PEDOT:PSS Films

Sordini

Garrudo

Rodrigues

et al. 2021

Front. Bioeng. Biotechnol.

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The ability to culture and differentiate neural stem cells (NSCs) to generate functional neural populations is attracting increasing attention due to its potential to enable cell-therapies to treat neurodegenerative diseases. Recent studies have shown that electrical stimulation improves neuronal differentiation of stem cells populations, highlighting the importance of the development of electroconductive biocompatible materials for NSC culture and differentiation for tissue engineering and regenerative medicine. Here, we report the use of the conjugated polymer poly(3,4-ethylenedioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS CLEVIOS P AI 4083) for the manufacture of conductive substrates. Two different protocols, using different cross-linkers (3-glycidyloxypropyl)trimethoxysilane (GOPS) and divinyl sulfone (DVS) were tested to enhance their stability in aqueous environments. Both cross-linking treatments influence PEDOT:PSS properties, namely conductivity and contact angle. However, only GOPS-cross-linked films demonstrated to maintain conductivity and thickness during their incubation in water for 15 days. GOPS-cross-linked films were used to culture ReNcell-VM under different electrical stimulation conditions (AC, DC, and pulsed DC electrical fields). The polymeric substrate exhibits adequate physicochemical properties to promote cell adhesion and growth, as assessed by Alamar Blue® assay, both with and without the application of electric fields. NSCs differentiation was studied by immunofluorescence and quantitative real-time polymerase chain reaction. This study demonstrates that the pulsed DC stimulation (1 V/cm for 12 days), is the most efficient at enhancing the differentiation of NSCs into neurons.

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Diphosphine‐Protected IrAu₁₂ Superatom with Open Site(s): Synthesis and Programmed Stepwise Assembly

Fukumoto,

Omoda,

Hirai

et al. 2024

Angewandte Chemie

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One or two phenylacetylide (PA) ligand(s) were successfully removed from the IrAu12 superatomic core of [IrAu12(dppe)5(PA)2]+ (dppe = 1,2‐bis(diphenylphosphino)ethane) by reaction with controlled amounts of tetrafluoroboric acid. Optical and nuclear magnetic resonance spectroscopies and density functional theory calculations revealed the formation of open Au site(s) on the IrAu12 core of [IrAu12(dppe)5(PA)1]2+ and [IrAu12(dppe)5]3+ with the remaining structure intact. Isocyanide was efficiently trapped at the open electrophilic site on [IrAu12(dppe)5(PA)1]2+, whereas a dimer or trimer of the IrAu12 superatoms was formed using diisocyanide as a linker. These results open the door to designed assembly of chemically modified metal superatoms.

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Polybenzimidazole nanofibers for neural stem cell culture

Cited by 20 publications

References 61 publications

PEDOT:PSS-Coated Polybenzimidazole Electroconductive Nanofibers for Biomedical Applications

PEDOT:PSS-Coated Polybenzimidazole Electroconductive Nanofibers for Biomedical Applications

Effect of Electrical Stimulation Conditions on Neural Stem Cells Differentiation on Cross-Linked PEDOT:PSS Films

Diphosphine‐Protected IrAu₁₂ Superatom with Open Site(s): Synthesis and Programmed Stepwise Assembly

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Polybenzimidazole nanofibers for neural stem cell culture

Cited by 20 publications

References 61 publications

PEDOT:PSS-Coated Polybenzimidazole Electroconductive Nanofibers for Biomedical Applications

PEDOT:PSS-Coated Polybenzimidazole Electroconductive Nanofibers for Biomedical Applications

Effect of Electrical Stimulation Conditions on Neural Stem Cells Differentiation on Cross-Linked PEDOT:PSS Films

Diphosphine‐Protected IrAu12 Superatom with Open Site(s): Synthesis and Programmed Stepwise Assembly

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Diphosphine‐Protected IrAu₁₂ Superatom with Open Site(s): Synthesis and Programmed Stepwise Assembly