Polyaniline/Single‐Walled Carbon Nanotubes Composite Based Triglyceride Biosensor

Abstract

Nanocomposite film comprising of polyaniline (PANI) and single walled carbon nanotubes (SWCNT) has been fabricated onto indium-tin-oxide (ITO) coated glass plate using electrophoretic technique. Co-immobilization of glycerol dehydrogenase (GDH) and lipase (LIP) has been done via N-ethyl-N'-(3-dimethylaminopropyl) carbodiimide and N-hydroxysuccinimide chemistry to explore its application for triglyceride (tributyrin) sensing. Response studies have been done using linear sweep voltammetry revealing that LIP-GDH/PANI-SWCNT-TB/ITO bioelectrode can detect tributyrin in the range of 50 to 400 mg dL À1 with low Michaelis-Menten constant of 1.138 mM, improved response time of 12 s, high sensitivity as 4.28 10 À4 mA mg À1 dL and storage stability of about 13 weeks.

Construction of a triglyceride amperometric biosensor based on chitosan–ZnO nanocomposite film

“…Recently considerable attention has been paid on the use of nanoparticles for improving biosensor, e.g. Prussian blue modified screen printed electrode [14], iridium nano-particle modified carbon paste electrode [15] polyaniline (PANI)/single-walled carbon nanotubes (SWCNTs) ITO coated glass plate [16].…”

Solvothermal synthesis of a polyaniline nanocomposite – a prospective biosensor electrode material

“…[5][6][7]. Consequently, researchers have combined functionalized CNTs with PANI [8,9] and evaluated their properties. However, as PANI is insoluble in water and common organic solvents, in order to improve its processability, researchers often carry out sulfonation in which sul- [10,11].…”

Synthesis and Optical Characterization of Sulfonated Polyaniline/Single-Walled Carbon Nanotube/Zinc Sulphide Nanocomposite

“…The CNTs have been extensively used as reinforcing fibers in PANI matrix to improve the characteristic optical and electrical properties of the polymer. The PANI/CNT composite finds application in biosensors [3], supercapacitors [4], metal-semiconductor devices [5], and actuators [6]. Zinc sulphide (ZnS) is a wide band gap compound semiconductor and has application as luminescent material [7,8].…”