Polyaniline-polycaprolactone blended nanofibers for neural cell culture

Garrudo, Fábio F. F.; Chapman, Caitlyn A.; Hoffman, Pauline R.; Udangawa, Ranodhi N.; Silva, João Carlos; Mikael, Paiyz E.; Rodrigues, Carlos Alberto Chirano; Cabral, Joaquim M. S.; Morgado, Jorge; Ferreira, Frederico Castelo; Linhardt, Robert J.

doi:10.1016/j.eurpolymj.2019.04.048

Cited by 65 publications

(36 citation statements)

References 46 publications

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“…The PCL-PANI spectrum showed all the main PCL characteristic peaks at 2942 and 2866 cm −1 , referring, respectively, to the asymmetric and symmetric stretching of CH2 groups [47], at 1721 and 1162 cm −1 , corresponding to the stretching of C=O [48] and C-O [49], and at 1294 cm −1 related to the bands of C-C and C-O of the PCL crystalline phase [50]. Moreover, specific PANI-CSA peaks at 1610, 1562 (stretching vibration of quinoid and benzenoid rings) [51] and 881 cm −1 (N-H bending of amine peak) [35] were also detected on the surface of PANI electrospun membranes.…”

Section: Ftir-atr Spectroscopymentioning

confidence: 99%

“…The obtained three-dimensional structure supported the infiltration and proliferation of bone marrow-derived mesenchymal stem cells (MSCs). Garrudo et al [ 35 ] investigated the attachment, viability and growth of neural stem cells (NSCs) seeded on PCL-PANI electrospun membranes for neural TE. Moreover, Hanumantharao et al [ 36 ] recently assessed the use of a PCL-PANI honeycomb electrospun scaffold for wound healing applications, by culturing adult human dermal fibroblast (HDFa) cells on the scaffold.…”

Section: Introductionmentioning

confidence: 99%

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Biocompatible Electrospun Polycaprolactone-Polyaniline Scaffold Treated with Atmospheric Plasma to Improve Hydrophilicity

2021

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Conductive polymers (CPs) have recently been applied in the development of scaffolds for tissue engineering applications in attempt to induce additional cues able to enhance tissue growth. Polyaniline (PANI) is one of the most widely studied CPs, but it requires to be blended with other polymers in order to be processed through conventional technologies. Here, we propose the fabrication of nanofibers based on a polycaprolactone (PCL)-PANI blend obtained using electrospinning technology. An extracellular matrix-like fibrous substrate was obtained showing a good stability in the physiological environment (37 °C in PBS solution up 7 days). However, since the high hydrophobicity of the PCL-PANI mats (133.5 ± 2.2°) could negatively affect the biological response, a treatment with atmospheric plasma was applied on the nanofibrous mats, obtaining a hydrophilic surface (67.1 ± 2°). In vitro tests were performed to confirm the viability and the physiological-like morphology of human foreskin fibroblast (HFF-1) cells cultured on the plasma treated PCL-PANI nanofibrous scaffolds.

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Section: Ftir-atr Spectroscopymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biocompatible Electrospun Polycaprolactone-Polyaniline Scaffold Treated with Atmospheric Plasma to Improve Hydrophilicity

2021

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“…Among the electroconductive materials available to produce such platforms, the most used are metals and organic materials, namely graphene, and conjugated polymers. Conjugated polymers present numerous advantages toward neural tissue engineering applications, namely: (1) they can be processed into any desired 3D-shape (Garrudo et al, 2019a , b ; Kayser and Lipomi, 2019 ); (2) can be easily functionalized allowing the tailoring of their mechanical, chemical, and electrical properties; (3) can exhibit high electroconductivity values, approaching those of metals; and (4) combine ionic and electronic conductivity, improving the “quality” of the interfaces with biological tissues (Rivnay et al, 2014 ; Inal et al, 2018 ; Goel et al, 2019 ; Pace et al, 2019 ). While electronic conductivity promotes higher current flow across the material, ion conductivity may prove to be essential to interface with tissues, as electrical signals in the human body are predominantly associated to ion currents.…”

Section: Introductionmentioning

confidence: 99%

Effect of Electrical Stimulation Conditions on Neural Stem Cells Differentiation on Cross-Linked PEDOT:PSS Films

Sordini

Garrudo

Rodrigues

et al. 2021

Front. Bioeng. Biotechnol.

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The ability to culture and differentiate neural stem cells (NSCs) to generate functional neural populations is attracting increasing attention due to its potential to enable cell-therapies to treat neurodegenerative diseases. Recent studies have shown that electrical stimulation improves neuronal differentiation of stem cells populations, highlighting the importance of the development of electroconductive biocompatible materials for NSC culture and differentiation for tissue engineering and regenerative medicine. Here, we report the use of the conjugated polymer poly(3,4-ethylenedioxythiophene) doped with polystyrene sulfonate (PEDOT:PSS CLEVIOS P AI 4083) for the manufacture of conductive substrates. Two different protocols, using different cross-linkers (3-glycidyloxypropyl)trimethoxysilane (GOPS) and divinyl sulfone (DVS) were tested to enhance their stability in aqueous environments. Both cross-linking treatments influence PEDOT:PSS properties, namely conductivity and contact angle. However, only GOPS-cross-linked films demonstrated to maintain conductivity and thickness during their incubation in water for 15 days. GOPS-cross-linked films were used to culture ReNcell-VM under different electrical stimulation conditions (AC, DC, and pulsed DC electrical fields). The polymeric substrate exhibits adequate physicochemical properties to promote cell adhesion and growth, as assessed by Alamar Blue® assay, both with and without the application of electric fields. NSCs differentiation was studied by immunofluorescence and quantitative real-time polymerase chain reaction. This study demonstrates that the pulsed DC stimulation (1 V/cm for 12 days), is the most efficient at enhancing the differentiation of NSCs into neurons.

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“…Akcoren et al prepared nanofiber mats from blends of polypyrrole and poly(butyl acrylate-co-methyl methacrylate), in this way, increasing the alternating current (AC) conductivity to a range of 0.4-0.5 µS/cm [33]. Poly(caprolactone)(PCL)/PAni nanofibers were electrospun using the camphorsulfonic acid doped green form of PAni by Garrudo et al, resulting in much higher conductivities in the range of 10 −4 -10 −1 S/cm [34]. Liu et al used a side-by-side spinneret to spin camphoric acid doped PAni together with PEO and found an increased spinnability combined with conductivities between 10 −6 and 10 −4 S/cm [35].…”

Section: Electrospinning From Conductive Solutions or Meltsmentioning

confidence: 99%

“…Rahmani et al used silk fibroin nanofibers filled with conductive reduced graphene oxide, resulting in electrochemical series resistances around 20-30 Ω, to grow conjunctiva mesenchymal stem cells under electrical stimulation and found formation of neuron-like cell morphology and alignment along the electrical field [72]. PCL/PAni scaffolds with conductivities up to approximately 80 µS/cm were used by Garrudo et al for the cultivation of neural stem cells, showing that the typical cell morphology was retained, and the nanofiber mats were biocompatible [34]. Even lower values of approximately 1 µS/cm were reported by Ghasemi et al who doped electrospun polyethylene terephthalate (PET) nanofibers with graphene oxide to prepare cardiac patches for cardiac regeneration after myocardial infarcts [73].…”

Section: Tissue Engineering and Cell Growthmentioning

confidence: 99%

Conductive Electrospun Nanofiber Mats

Błachowicz

Ehrmann

2019

Materials

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Conductive nanofiber mats can be used in a broad variety of applications, such as electromagnetic shielding, sensors, multifunctional textile surfaces, organic photovoltaics, or biomedicine. While nanofibers or nanofiber from pure or blended polymers can in many cases unambiguously be prepared by electrospinning, creating conductive nanofibers is often more challenging. Integration of conductive nano-fillers often needs a calcination step to evaporate the non-conductive polymer matrix which is necessary for the electrospinning process, while conductive polymers have often relatively low molecular weights and are hard to dissolve in common solvents, both factors impeding spinning them solely and making a spinning agent necessary. On the other hand, conductive coatings may disturb the desired porous structure and possibly cause problems with biocompatibility or other necessary properties of the original nanofiber mats. Here we give an overview of the most recent developments in the growing field of conductive electrospun nanofiber mats, based on electrospinning blends of spinning agents with conductive polymers or nanoparticles, alternatively applying conductive coatings, and the possible applications of such conductive electrospun nanofiber mats.

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Polyaniline-polycaprolactone blended nanofibers for neural cell culture

Cited by 65 publications

References 46 publications

Biocompatible Electrospun Polycaprolactone-Polyaniline Scaffold Treated with Atmospheric Plasma to Improve Hydrophilicity

Biocompatible Electrospun Polycaprolactone-Polyaniline Scaffold Treated with Atmospheric Plasma to Improve Hydrophilicity

Effect of Electrical Stimulation Conditions on Neural Stem Cells Differentiation on Cross-Linked PEDOT:PSS Films

Conductive Electrospun Nanofiber Mats

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